What is Dulbecco's Phosphate Buffered Saline (DPBS)?

Dulbecco's Phosphate Buffered Saline (DPBS) is a saline solution that maintains the pH and osmotic balance in biological research. This specific formulation is free from calcium and magnesium ions, making it suitable for various cell culture applications. It serves as a transport medium for cells and tissues, and is commonly used in laboratories for rinsing cells, diluting substances, and resuspending cells prior to further experiments.

How do I use Dulbecco's Phosphate Buffered Saline in my laboratory?

To use DPBS, simply measure the required volume and pour it into your petri dish or cell culture flask. It can be utilized to wash cells, prepare samples, or maintain osmotic balance during experiments. Be sure to adhere to sterile techniques while handling, as contamination can affect experimental results. For example, you might use it to rinse adherent cell cultures before trypsinization or during cell count procedures.

Can I store Dulbecco's Phosphate Buffered Saline, and how?

Yes, DPBS should be stored at room temperature, away from direct sunlight, and used within a certain timeframe after opening to maintain its sterility and efficacy. Proper storage helps preserve the quality of the buffer, ensuring reliable results in experiments. For best practices, consider sealing the bottle securely after use to prevent contamination, and label with the opening date to keep track of its usability.

Is Dulbecco's Phosphate Buffered Saline suitable for all types of cells?

While DPBS is widely used for many cell types, its suitability may vary depending on specific cellular requirements. Its lack of calcium and magnesium makes it appropriate for certain applications like rinsing or transporting cells without causing activation or aggregation. Always check the compatibility with your specific cell line or application to confirm it's the right choice, especially for sensitive primary cells.

What is the significance of the pH level in Dulbecco's Phosphate Buffered Saline?

The pH level of 7.4 in DPBS is crucial for maintaining physiological conditions that support cell viability and function. Deviations in pH can lead to cellular stress and affect experimental outcomes. By ensuring a stable pH, researchers can create an environment that mimics conditions in the human body, which is particularly important in cell culture and drug testing.

What does '0.1 m Filtered Sterilization' mean in the product?

The term '0.1 m Filtered Sterilization' indicates that the DPBS solution has been filtered through a 0.1-micron filter, effectively removing any bacteria or particulate matter, thereby ensuring sterility. This process is essential for laboratory work where contamination could compromise results. Using pre-filtered solutions can save time and effort in preparing sterile media, facilitating cleaner experimental conditions.

Can Dulbecco's Phosphate Buffered Saline be used for cell preservation?

While DPBS is primarily used for cell rinsing and transport, it is not ideal for long-term cell preservation. Instead, it can be used temporarily to maintain cells in culture before transferring them to preservation media like DMSO or glycerol. In practice, you might use DPBS to wash cells before cryopreservation, ensuring they are in optimized conditions for freezing.

What applications are best suited for Dulbecco's Phosphate Buffered Saline?

DPBS is frequently used in a variety of applications such as cell culture, tissue processing, and in vitro diagnostic testing. Its properties make it ideal for washing cells, diluting biochemical assays, and maintaining osmotic balance. For instance, during immunostaining procedures, DPBS can help facilitate the washing steps without interfering with the binding of antibodies.

Is Dulbecco's Phosphate Buffered Saline available without preservatives?

Yes, Dulbecco's Phosphate Buffered Saline is typically offered in a formulation that does not contain preservatives, ensuring that it does not affect cell growth or experimental results. When choosing a saline buffer for sensitive experiments, it’s critical to select products that are free from additives that could interfere with your assays or cell lines. Therefore, always double-check the product specifications to confirm its composition.

Where can I buy Dulbecco's Phosphate Buffered Saline in South Africa?

You can conveniently purchase Dulbecco's Phosphate Buffered Saline from Ubuy in South Africa. Ubuy offers a wide range of laboratory supplies and ensures that you have easy access to high-quality products like DPBS. Their platform is user-friendly, and you can typically find various brands and formulations, giving you options suited to your specific laboratory needs.

Is Dulbecco's Phosphate Buffered Saline, 1X DPBS Buffer Available to Shop Online in South Africa?

Yes, at Ubuy South Africa this product is available for you to shop at a reasonable price. The Dulbecco's Phosphate Buffered Saline, 1X DPBS Buffer is not available locally but you can trust us with our express shipping services.

How Long Does It Take to Get Product After Placing the Order?

The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

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Dulbecco's Phosphate Buffered Saline, 1X DPBS Buffer Without Calcium and Magnesium, pH 7.4, 0.1m Filtered Sterilization, 500ml/Bottle, 20 Bottle/Case

Designed for immediate use, our PBS solution is pre-prepared and ready to go right out of the bottle—no need for preparation, dilution, or additional handling.

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Item #: 220674748

Dulbecco's Phosphate Buffered Saline, 1X DPBS Buffer Without Calcium and Magnesium, pH 7.4, 0.1m Filtered Sterilization, 500ml/Bottle, 20 Bottle/Case

Item #: 220674748

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Dulbecco's Phosphate Buffered Saline, 1X DPBS Buffer Without Calcium and Magnesium, pH 7.4, 0.1m Filtered Sterilization, 500ml/Bottle, 20 Bottle/Case

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Question: Is Dulbecco's Phosphate Buffered Saline, 1X DPBS Buffer Available to Shop Online in South Africa?

Answer: Yes, at Ubuy South Africa this product is available for you to shop at a reasonable price. The Dulbecco's Phosphate Buffered Saline, 1X DPBS Buffer is not available locally but you can trust us with our express shipping services.
Question: How Long Does It Take to Get Product After Placing the Order?

Answer: The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

CryoKing Buffers Editorial Review

This product has garnered a favorable reception among users, highlighted by Consistent praise for its performance in specific research applications. Customers report that it functions as described, proving effective in vital processes such as reConstituting compounds like ARA-290 and TA-1 without introducing contamination, which is crucial in research settings. Many users noted the product's substantial size, indicating that it provides excellent value for the price. Additionally, it has been positively reviewed for its compatibility with peptides, further solidifying its reputation as a reliable choice for researchers looking to work with these compounds. Overall, this product stands out in terms of quality, effectiveness, and value, making it a commendable option for anyone in need of a dependable mixing solution for their research applications. **

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Functions as described without contamination.
Effective for reConstituting various compounds, including ARA-290 and TA-1.
Large bottle size offers excellent value for the price.
Good compatibility with peptides.

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Limitations : For products shipped internationally, please note that any manufacturer warranty may not be valid; manufacturer service options may not be available; product manuals, instructions, and safety warnings may not be in destination country languages; the products (and accompanying materials) may not be designed in accordance with destination country standards, specifications, and labeling requirements; and the products may not conform to destination country voltage and other electrical standards (requiring use of an adapter or converter if appropriate). The recipient is responsible for assuring that the product can be lawfully imported to the destination country. When ordering from Ubuy or its affiliates, the recipient is the importer of record and must comply with all laws and regulations of the destination country.
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Features & Benefits

Advanced Technology: pH=7.4±0.1, sterilized for reliability.
No Calcium & Magnesium: Prevents interference in sensitive experiments.
Widespread Use: Ideal for cell dissociation and tissue sample transportation.
Ready-to-Use: Eliminates preparation time for streamlined workflows.
Long Shelf Life: 3 years at room temperature for ongoing research needs.
Consistent Results: Ensures controlled ion conditions for reliable outcomes.

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Dulbecco's Phosphate Buffered Saline, 1X DPBS Buffer Without Calcium and Magnesium, pH 7.4, 0.1m Filtered Sterilization, 500ml/Bottle, 20 Bottle/Case

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