What is PBST (PBS-Tween 20) used for?

PBST, or PBS-Tween 20, is commonly used in biological and biochemical research. It acts as a buffer solution that maintains a stable pH and is particularly effective in wash steps during cell and protein assays. The addition of Tween 20, a non-ionic detergent, reduces non-specific binding of proteins to surfaces, improving the clarity and accuracy of experimental results. This makes PBST particularly valuable in applications like ELISA, Western blotting, and immunohistochemistry.

What are the components of PBST?

PBST consists of phosphate-buffered saline (PBS) and Tween 20. PBS is an isotonic buffer containing sodium chloride, potassium chloride, sodium phosphate, and potassium phosphate, which helps maintain osmotic balance. Tween 20 is a surfactant that helps reduce protein aggregation and facilitates better penetration in assays, leading to more reliable outcomes. Together, these components optimize conditions for various biological applications.

How do you prepare PBST from the 10X stock solution?

To prepare PBST from the 10X stock solution, simply dilute the stock solution in distilled water to reach a final concentration. For example, mix one part of the 10X PBST with nine parts of distilled water to achieve a 1X solution. This dilution ensures that the pH and buffer capacity of the solution are maintained, allowing for optimal performance in your assays. Always check the pH as a best practice to ensure it remains around 7.4.

What is the significance of the pH 7.4 in PBST?

The pH of 7.4 in PBST is critical as it closely matches the physiological pH of most biological systems. This neutral pH ensures that proteins and cells remain stable and functional during experimental processes. Maintaining this pH level can greatly influence the efficiency of antibody binding and protein interactions, making it vital in experiments such as immunoassays where biological relevance is key.

Can PBST be stored for later use?

Yes, PBST can be stored for future use, provided it is kept under appropriate conditions. Store the solution at 4°C to maintain its stability and effectiveness. However, it’s advisable to avoid repeated freeze-thaw cycles which can degrade the solution's components. When stored correctly, PBST can remain effective for several weeks, making it convenient for multiple experiments.

Is PBST safe to use in a laboratory environment?

PBST is generally considered safe for use in laboratory environments, with no known toxic effects from its components. However, like any reagent, it should be handled with care, using appropriate personal protective equipment, such as gloves and goggles. It is always good practice to review the safety data sheet (SDS) for proper handling guidelines to ensure a safe working environment.

What are some common applications of PBST in research?

PBST is widely used in various research applications, including ELISA assays for quantitative analysis of proteins and Western blotting for the detection of specific proteins. Moreover, it is beneficial in immunocytochemistry techniques to detect antigens in cells and tissues. Its role in these applications enhances the reliability and clarity of results, crucial for scientific studies.

How does the presence of Tween 20 affect experimental outcomes?

The inclusion of Tween 20 in PBST enhances experimental outcomes by minimizing non-specific binding and improving the accessibility of antibodies to their targets. This surfactant disperses proteins and helps maintain their stability, resulting in clearer assay results. Consequently, using PBST with Tween 20 can significantly enhance sensitivity and specificity in applications such as ELISA and Westerns.

What is the shelf-life of PBST once opened?

Once opened, PBST should ideally be used within a certain timeframe to ensure its effectiveness, typically within 2-4 weeks when stored at 4°C. The shelf-life can be affected by factors such as contamination or exposure to light. To maximize its usability, it is recommended to avoid touching the inside of the cap or the opening of the bottle, ensuring the integrity of the solution.

Where can I buy PBST (PBS-Tween 20), 10X, pH 7.4, 500ml/Unit in South Africa?

You can purchase PBST (PBS-Tween 20), 10X, pH 7.4, 500ml/Unit from Ubuy in South Africa. Ubuy offers a variety of laboratory reagents and supplies with the convenience of online shopping, ensuring you can easily find and acquire the essential products you need for your research.

How to Shop PBST (PBS-Tween 20), 10X, pH 7.4, 1000ml/Unit Online From Ubuy?

It’s easy to shop PBST (PBS-Tween 20), 10X, pH 7.4, 1000ml/Unit online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.

Is PBST (PBS-Tween 20), 10X, pH 7.4, 1000ml/Unit Available to Shop Online in South Africa?

Yes, at Ubuy South Africa this product is available for you to shop at a reasonable price. The PBST (PBS-Tween 20), 10X, pH 7.4, 1000ml/Unit is not available locally but you can trust us with our express shipping services.

How Long Does It Take to Get Product After Placing the Order?

The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

APEX

PBST (PBS-Tween 20), 10X, pH 7.4, 1000ml/Unit

Apex Liquid Buffers are crafted using ultra pure or cGMP grade chemicals, ensuring high quality for all your molecular biology needs.

APEX

Item #: 181073531

PBST (PBS-Tween 20), 10X, pH 7.4, 1000ml/Unit

Item #: 181073531

ZAR 2610

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What Stands Out

Concentrated Solution

This 10X concentrated PBS-Tween 20 solution saves storage space and minimizes shipping costs, providing an efficient and economical option for laboratories requiring high-quality reagents.

Ideal pH Balance

With a precise pH of 7.4, this solution mimics physiological conditions, ensuring optimal performance in various biological applications, enhancing reproducibility in your experiments.

Bulk Quantity

The 500ml unit size is perfect for research labs, allowing for prolonged use without frequent reorders, ensuring uninterrupted research workflows and productivity.

Product Details

Item Weight

1.1 lbs (500 grams)

Who Should Buy?

Laboratory Researchers
Ideal for researchers needing a reliable buffer solution for biological experiments and assays requiring precise pH conditions.
Diagnostic Facilities
Perfect for assay development and standard testing, providing consistency for numerous diagnostic procedures in clinical settings.
Biotechnology Companies
Useful for companies conducting enzyme assays, Western blots, and immunoassays, ensuring accurate experimental conditions.

Home Users
Not suitable for general home use or non-scientific applications, as it's specifically designed for laboratory settings.
Non-Scientific Applications
Inappropriate for consumers looking for cleaning or household products due to its specialized buffer composition.
Beginner Experimentation
May not fit novice users without prior experience in handling biochemical solutions, requiring more basic alternatives.

Product Description

PBST (PBS-Tween 20), 10X, pH 7.4, 1000ml/Unit

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Customer Questions & Answers

Question: How to Shop PBST (PBS-Tween 20), 10X, pH 7.4, 1000ml/Unit Online From Ubuy?

Answer: It’s easy to shop PBST (PBS-Tween 20), 10X, pH 7.4, 1000ml/Unit online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.
Question: Is PBST (PBS-Tween 20), 10X, pH 7.4, 1000ml/Unit Available to Shop Online in South Africa?

Answer: Yes, at Ubuy South Africa this product is available for you to shop at a reasonable price. The PBST (PBS-Tween 20), 10X, pH 7.4, 1000ml/Unit is not available locally but you can trust us with our express shipping services.
Question: How Long Does It Take to Get Product After Placing the Order?

Answer: The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

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Limitations : For products shipped internationally, please note that any manufacturer warranty may not be valid; manufacturer service options may not be available; product manuals, instructions, and safety warnings may not be in destination country languages; the products (and accompanying materials) may not be designed in accordance with destination country standards, specifications, and labeling requirements; and the products may not conform to destination country voltage and other electrical standards (requiring use of an adapter or converter if appropriate). The recipient is responsible for assuring that the product can be lawfully imported to the destination country. When ordering from Ubuy or its affiliates, the recipient is the importer of record and must comply with all laws and regulations of the destination country.
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Features & Benefits

Uses ultra pure or cGMP grade chemicals for maximum quality.
Contains 4 fold purified water for enhanced performance.
Ideal for molecular biology research and protein analysis.
Purity exceeds 99%, ensuring reliable results.
Free from RNase, DNase, endonucleases, endotoxins, proteins, and proteases.
Perfect for purification, crystallization, and diagnostic applications.

PBST (PBS-Tween 20), 10X, pH 7.4, 1000ml/Unit

ZAR 2610

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Secure Packaging

100% Original Products

PCI DSS Compliance

ISO 27001 Certified

What Stands Out

Product Details

Who Should Buy?

Product Description

Customer Questions & Answers

APEX Buffers Editorial Review

Customer Reviews & Ratings

Review this product

Product Price History

Important information

ZAR 2610

Features & Benefits

Fast
Shipping

Free
Return^*