What is the MACHEREY-NAGEL Nucleosil 100-5 C8 EC HPLC Column used for?

The MACHEREY-NAGEL Nucleosil 100-5 C8 EC HPLC Column is designed for high-performance liquid chromatography applications. It's particularly suited for separating complex mixtures, making it ideal for pharmaceutical, environmental, and chemical analysis. The unique C8 stationary phase provides a balance of polar and non-polar interactions, enhancing separation efficiency for various compounds. Laboratories commonly use this column to achieve high-resolution separations in their analytical workflows, ensuring accurate results in both qualitative and quantitative analyses.

What are the dimensions of the MACHEREY-NAGEL 720165.46 column?

The MACHEREY-NAGEL 720165.46 Nucleosil 100-5 C8 EC HPLC column features dimensions of 250 mm in length and 4.6 mm in inner diameter (ID). These specifications make it suitable for standard analytical applications, allowing for efficient sample loading and effective separation. The 250 mm length ensures a greater interaction surface area, which is pivotal in achieving high-resolution results in HPLC processes. This column is compatible with a variety of HPLC systems, making it a versatile choice for different laboratory setups.

What does USP L7 classification mean in relation to this HPLC column?

The USP L7 classification indicates that the MACHEREY-NAGEL Nucleosil 100-5 C8 EC HPLC column meets specific performance criteria set by the United States Pharmacopeia for C8 columns. This classification denotes that the column provides reliable and reproducible results for non-polar analytes, essential for pharmaceutical applications. The USP grading provides confidence to users about the column's performance, particularly in quality control and method development situations where precision and accuracy are critical.

Can the MACHEREY-NAGEL Nucleosil 100-5 C8 EC column be used for different types of samples?

Yes, the MACHEREY-NAGEL Nucleosil 100-5 C8 EC HPLC column is versatile and can be used effectively with a wide range of sample types, including small organic molecules, peptides, and even some proteins. Its C8 stationary phase helps in optimizing the separation of various compound classes, making it a popular choice in various fields like pharmaceuticals, food safety, and environmental testing. Researchers often switch between different sample matrices while continuing to obtain high-quality results with this column.

What is the typical operating pressure range for this HPLC column?

The MACHEREY-NAGEL 720165.46 HPLC column typically operates within a pressure range of 40 to 120 bar. Operating within this pressure range ensures optimal flow rates and separation efficiencies without compromising the integrity of the column. It’s essential to monitor the pressure during operation; exceeding the recommended levels can lead to column damage or compromised separation performance. Users should regularly check their HPLC setup to maintain the ideal conditions for analysis.

Is the column suitable for gradient elution methods?

Yes, the MACHEREY-NAGEL Nucleosil 100-5 C8 EC HPLC column is well-suited for gradient elution methods. Its design facilitates the effective separation of compounds under changing solvent conditions. Gradient elution can enhance separation efficiency, especially for complex mixtures, as it helps to reduce retention times and improve peak shape. This feature is particularly beneficial in high-throughput laboratories where time savings without sacrificing resolution are crucial.

What mobile phases work best with this HPLC column?

This HPLC column performs optimally with a range of mobile phases, typically including mixtures of water with organic solvents like acetonitrile or methanol. The choice of mobile phase greatly influences resolution and retention times, meaning users can customize their method based on specific analyte characteristics. Depending on the target compounds' polarities, fine-tuning the mobile phase composition can lead to significant improvements in separation quality, which is essential for achieving reliable analytical results.

How should the column be maintained for best performance?

To maintain the optimal performance of the MACHEREY-NAGEL Nucleosil 100-5 C8 EC HPLC column, it's crucial to follow proper care protocols. This includes regularly flushing the column with appropriate solvents to remove any residual materials and minimizing exposure to extreme conditions that could degrade the stationary phase. Additionally, ensure that the mobile phase is filtered and degassed to prevent contamination and bubble formation during analysis. Such maintenance practices help in prolonging the column's lifespan and ensuring consistently high-quality results.

Are there specific applications where this HPLC column excels?

The MACHEREY-NAGEL Nucleosil 100-5 C8 EC HPLC column excels in a variety of applications, particularly in pharmaceutical analysis, due to its ability to provide reliable separation of active pharmaceutical ingredients (APIs) and related substances. It's also widely used in environmental testing for the separation of pollutants and in food safety for analyzing contaminants. Laboratories often rely on this column for method development and validation in compliance with regulatory standards, making it a trusted choice in many analytical settings.

How to Shop MACHEREY-NAGEL 720165.46 Nucleosil 100-5 C8 EC HPLC Online From Ubuy?

It’s easy to shop MACHEREY-NAGEL 720165.46 Nucleosil 100-5 C8 EC HPLC online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.

Is MACHEREY-NAGEL 720165.46 Nucleosil 100-5 C8 EC HPLC Available to Shop Online in South Africa?

Yes, at Ubuy South Africa this product is available for you to shop at a reasonable price. The MACHEREY-NAGEL 720165.46 Nucleosil 100-5 C8 EC HPLC is not available locally but you can trust us with our express shipping services.

How Long Does It Take to Get Product After Placing the Order?

The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

MACHEREY-NAGEL

NUCLEOSIL is one of the first spherical silicas used in HPLC, widely accepted as routine packing for very different fields of chromatography.

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Item #: 145590846

MACHEREY-NAGEL 720165.46 Nucleosil 100-5 C8 EC HPLC 1 Column, Analytical, 250 mm Length, 4.6 mm ID, USP L7

Q: Where can I buy MACHEREY-NAGEL 720165.46 Nucleosil 100-5 C8 EC HPLC 1 Column in South Africa?

You can purchase the MACHEREY-NAGEL 720165.46 Nucleosil 100-5 C8 EC HPLC column from Ubuy in South Africa. Ubuy is a reputable online platform that offers a wide range of laboratory products, including analytical columns, ensuring that you can find what you need conveniently. By visiting their website, you can explore product details, availability, and other related items to support your laboratory needs.

Item #: 145590846

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NUCLEOSIL is one of the first spherical silicas used in HPLC, widely accepted as routine packing for very different fields of chromatography.

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What Stands Out

High Efficiency

The MACHEREY-NAGEL Nucleosil 100-5 C8 column ensures exceptional separation efficiency, making it ideal for analytical applications requiring precise results in high-performance liquid chromatography.

Versatile Applications

Designed for a wide range of samples, this HPLC column excels in various analytical processes, catering to diverse industries from pharmaceuticals to environmental testing.

Dependable Quality

Manufactured under stringent quality controls, this column guarantees consistent performance and reliability, enhancing laboratory throughput while reducing method development time.

Product Details

Item Weight

0.5 lbs (230 grams)

Who Should Buy?

Analytical Chemists
Perfect for analytical chemists focusing on method development and compound separation in complex mixtures.
Pharmaceutical Research
Ideal for pharmaceutical labs needing reliable and reproducible HPLC results for drug analysis.
Quality Control Labs
Suited for quality control labs requiring consistent performance in testing the purity of substances.

Beginners
Not suitable for beginners without prior knowledge of HPLC systems and column selection processes.
Large Volume Samples
Designed for analytical applications, may not be appropriate for large volume sample analysis.
Non-Polar Solvents
Not ideal for methods involving non-polar solvents; best performance is with polar and moderately polar compounds.

Product Description

MACHEREY-NAGEL 720165.46 Nucleosil 100-5 C8 EC HPLC 1 Column, Analytical, 250 mm Length, 4.6 mm ID, USP L7

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Question: How to Shop MACHEREY-NAGEL 720165.46 Nucleosil 100-5 C8 EC HPLC Online From Ubuy?

Answer: It’s easy to shop MACHEREY-NAGEL 720165.46 Nucleosil 100-5 C8 EC HPLC online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.
Question: Is MACHEREY-NAGEL 720165.46 Nucleosil 100-5 C8 EC HPLC Available to Shop Online in South Africa?

Answer: Yes, at Ubuy South Africa this product is available for you to shop at a reasonable price. The MACHEREY-NAGEL 720165.46 Nucleosil 100-5 C8 EC HPLC is not available locally but you can trust us with our express shipping services.
Question: How Long Does It Take to Get Product After Placing the Order?

Answer: The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

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Features & Benefits

Totally porous spherical silicas with a pure SiO2 structure.
End capped with a pore size of 100 Å and particle size of 5 µm.
Separation mechanism based on hydrophobic interactions.
Minimal residual silanol interactions for improved performance.
Highly regarded for diverse applications in chromatography.
Reliable choice for HPLC as a routine packing material.

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MACHEREY-NAGEL 720165.46 Nucleosil 100-5 C8 EC HPLC 1 Column, Analytical, 250 mm Length, 4.6 mm ID, USP L7

ZAR 19875

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