What is the Omicron S045A330XI Nitrocellulose Transfer Blotting Membrane used for?

The Omicron S045A330XI Nitrocellulose Transfer Blotting Membrane is primarily used in molecular biology procedures for transferring proteins from gels to membranes after electrophoresis. This step is crucial for subsequent analysis techniques like Western blotting, immunodetection, and protein analysis, allowing researchers to detect specific proteins with high sensitivity. The 0.45-micron pore size facilitates optimal binding of proteins, making it ideal for various applications in laboratories focusing on protein research and diagnostics.

What are the dimensions of the Omicron S045A330XI Nitrocellulose Transfer Blotting Membrane?

The Omicron S045A330XI Nitrocellulose Transfer Blotting Membrane comes in a substantial size of 300mm x 3m. This ample dimension allows users to cut out pieces that suit their specific experiments and multiple applications. Whether for large-scale protein analysis or smaller experiments, the generous roll length ensures laboratory efficiency by minimizing waste while accommodating different gel sizes and applications.

Can the Omicron Nitrocellulose Membrane be used for both SDS-PAGE and native protein transfers?

Yes, the Omicron Nitrocellulose Membrane is versatile and can be used for both SDS-PAGE and native protein transfers. The membrane's intact structure effectively retains proteins during the transfer process, making it suitable for various conditions. Researchers frequently opt for this membrane to analyze both denatured proteins, which gives insights about protein structure, and native proteins, to explore activity and interactions in their natural state, enhancing experimental flexibility.

How do you handle the Omicron Nitrocellulose Membrane during transfer?

When handling the Omicron Nitrocellulose Membrane, it is essential to wear gloves to prevent contamination and degradation. Carefully place the membrane on top of the gel ensuring a bubble-free contact for optimal protein binding. The transfer device should be calibrated correctly to maintain consistent pressure for effective protein transfer. Proper handling and preparation are vital in ensuring accurate results, making this process critical for researchers engaged in protein studies.

What is the shelf life of the Omicron Nitrocellulose Transfer Membrane?

The shelf life of the Omicron Nitrocellulose Transfer Membrane is generally determined by storage conditions. When stored in a cool, dry place and sealed properly, its longevity is significantly extended. Users are advised to check the packaging for specific storage instructions. Understanding shelf life is important for laboratories to ensure they utilize high-quality materials, thereby ensuring the reliability of experimental results and data.

How do you pre-wet the Omicron Nitrocellulose Membrane before use?

Before using the Omicron Nitrocellulose Membrane, it is crucial to pre-wet it in methanol or a transfer buffer for optimal protein binding. Immerse the membrane completely for a few seconds until it becomes uniformly soaked. This step activates the membrane's surface and enhances protein adsorption, thereby improving the overall sensitivity during analysis. Proper pre-wetting ensures that the proteins transfer efficiently and reduces variability in results across different experiments.

Is the Omicron Nitrocellulose Membrane compatible with all protein assays?

The Omicron Nitrocellulose Membrane is compatible with a wide range of protein assays, particularly Western blot analyses, enzyme-linked immunosorbent assays (ELISA), and other immunodetection techniques. However, it is essential for users to consider the protein's size and characteristics when selecting assays, as this can impact binding efficiency. Researchers often choose this membrane for its broad compatibility, ensuring versatility in various analytical applications.

Can the Omicron Nitrocellulose Membrane be reused after initial use?

Generally, the Omicron Nitrocellulose Membrane is designed for single use to ensure the integrity and reliability of results. Reusing the membrane can lead to cross-contamination and compromised experimental outcomes. Laboratories often prioritize obtaining fresh membranes to maintain the quality of their assays. However, in some cases, researchers may explore alternative methods when reusability is essential; nonetheless, discretion is advised.

How do you ensure optimal protein transfer with the Omicron Nitrocellulose Membrane?

To ensure optimal protein transfer with the Omicron Nitrocellulose Membrane, it is critical to adjust transfer conditions like voltage, duration, and buffer composition according to the protein sizes. Conducting test runs can help optimize these parameters based on specific experiments. Additionally, following protocols and guidelines for different types of proteins can enhance efficiency. For instance, larger proteins may require longer transfer times, making careful planning necessary for achieving successful results.

Where can I buy the Omicron S045A330XI Nitrocellulose Transfer Blotting Membrane in South Africa?

You can purchase the Omicron S045A330XI Nitrocellulose Transfer Blotting Membrane from Ubuy in South Africa. Ubuy is a reputable online platform known for its extensive product range, including laboratory supplies. You can conveniently browse, select, and order the product directly through their website, ensuring a hassle-free shopping experience tailored specifically for your needs.

How to Shop OmicronTM S045A330XI Nitrocellulose Online From Ubuy?

It’s easy to shop OmicronTM S045A330XI Nitrocellulose online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.

Is OmicronTM S045A330XI Nitrocellulose Available to Shop Online in South Africa?

Yes, at Ubuy South Africa this product is available for you to shop at a reasonable price. The OmicronTM S045A330XI Nitrocellulose is not available locally but you can trust us with our express shipping services.

How Long Does It Take to Get Product After Placing the Order?

The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

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100% pure nitrocellulose membrane validated for all protein blotting applications.

Omicron

Item #: 121832799

OmicronTM S045A330XI Nitrocellulose Transfer(Blotting Membrane), 300mmX3m Roll, 0.45 Micron (Cross Reference 11306-41BL)

Item #: 121832799

ZAR 6909

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What Stands Out

High-Quality Material

Utilizes premium nitrocellulose for superior transfer efficiency and sharp results, ensuring reliable detection of proteins and nucleic acids in various applications.

Optimal Pore Size

With a 0.45 micron pore size, this membrane facilitates improved flow rates and enhances the interaction of samples for better visualization during the blotting process.

Convenient Size

A generous 300mm x 3m roll allows for versatile applications, reducing wastage and providing ample material for multiple experiments or different workflows.

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Item Weight

0.5 lbs (230 grams)

Who Should Buy?

Molecular Biologists
Ideal for researchers needing high-quality membranes for protein and nucleic acid transfer during blotting procedures.
Laboratory Technicians
Suitable for routine applications in labs requiring reliable and efficient transfer solutions for multiple assays.
Educational Institutions
Perfect for teaching labs where students learn techniques for protein analysis and molecular biology fundamentals.

Budget-Conscious Users
May not be suitable for users seeking cost-effective solutions; premium pricing could be a limitation.

Product Description

OmicronTM S045A330XI Nitrocellulose Transfer(Blotting Membrane), 300mmX3m Roll, 0.45 Micron (Cross Reference 11306-41BL)

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Question: How to Shop OmicronTM S045A330XI Nitrocellulose Online From Ubuy?

Answer: It’s easy to shop OmicronTM S045A330XI Nitrocellulose online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.
Question: Is OmicronTM S045A330XI Nitrocellulose Available to Shop Online in South Africa?

Answer: Yes, at Ubuy South Africa this product is available for you to shop at a reasonable price. The OmicronTM S045A330XI Nitrocellulose is not available locally but you can trust us with our express shipping services.
Question: How Long Does It Take to Get Product After Placing the Order?

Answer: The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

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Features & Benefits

100% pure nitrocellulose for superior quality.
Validated for all protein blotting applications.
Hydrophilic with high protein/nucleic acid binding capacity.
Ideal for disease diagnosis including HIV and Hepatitis B.
Offers excellent sensitivity and low background for detection systems.
Custom sizes available upon request.

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OmicronTM S045A330XI Nitrocellulose Transfer(Blotting Membrane), 300mmX3m Roll, 0.45 Micron (Cross Reference 11306-41BL)

ZAR 6909

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ZAR 6909

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