What is 20x SSPE Buffer and how is it used?

20x SSPE Buffer is a high-concentration solution used primarily in molecular biology for various applications such as hybridization and washing of nucleic acid blots. This buffer provides an ideal ionic environment, enhancing the stability of DNA and RNA interactions during experiments. It's especially useful in procedures like Southern and Northern blotting, where high salt concentration helps maintain strand associations. By diluting it to a working concentration, researchers can optimize conditions for their experiments effectively.

What are the components of 20x SSPE Buffer?

20x SSPE Buffer typically consists of sodium chloride, sodium phosphate, and EDTA. These components are crucial for maintaining the appropriate ionic strength and pH conditions during molecular biology applications. Sodium chloride stabilizes nucleic acid interactions, while sodium phosphate adjusts the pH, allowing for optimal hybridization conditions. EDTA chelates divalent metal ions that could otherwise degrade nucleic acids, ensuring sample integrity for accurate results.

How should 20x SSPE Buffer be stored?

20x SSPE Buffer should be stored at a temperature of 4°C for short-term use and can be frozen at -20°C for long-term storage. When storing, it’s advisable to use airtight containers to prevent contamination and degradation from moisture and light. Regularly checking for any signs of precipitation or discoloration can ensure the buffer remains effective for experiments. Proper storage will help maintain its stability and reliability in various laboratory applications.

Can 20x SSPE Buffer be used for DNA gel electrophoresis?

While 20x SSPE Buffer is primarily designed for hybridization applications, it is not typically used as a running buffer for DNA gel electrophoresis. Instead, running buffers like TAE or TBE are preferred, as they provide better separation capabilities for nucleic acids. However, SSPE can be useful in post-electrophoresis applications, such as in southern blotting, to help maintain DNA stability during the transfer and hybridization processes.

What dilution is required to prepare a working solution from 20x SSPE Buffer?

To prepare a working solution from 20x SSPE Buffer, dilution to a 1x concentration is required. This means mixing one part of the 20x buffer with 19 parts of distilled water or an appropriate diluent. For example, to create 100 mL of 1x SSPE, you would add 5 mL of 20x buffer to 95 mL of water. This diluted solution can then be used in various downstream applications, enhancing the effectiveness of nucleic acid assays.

Is 20x SSPE Buffer suitable for use in RNA experiments?

Yes, 20x SSPE Buffer is suitable for RNA experiments and is particularly useful in applications such as RNA blotting and hybridization. Its high salt concentration helps maintain RNA stability and prevents the degradation of RNA during experimental procedures. In scenarios where high specificity in hybridization is essential, using 20x SSPE can enhance the signal and reduce background noise by providing optimal ionic conditions for RNA binding.

What role does EDTA play in 20x SSPE Buffer?

EDTA in 20x SSPE Buffer serves as a chelating agent that binds divalent metal ions like magnesium and calcium, which can catalyze the degradation of nucleic acids. By sequestering these ions, EDTA enhances the stability of both DNA and RNA during laboratory procedures, minimizing the risk of enzymatic breakdown. This is particularly important in applications like hybridization, where the integrity of nucleic acids is crucial for reliable results.

How can you utilize 20x SSPE Buffer in PCR applications?

While 20x SSPE Buffer is not typically used directly in PCR, it can be beneficial in the post-amplification steps, such as purification and probe hybridization. It provides a stable ionic environment for maintaining the integrity of PCR products. After amplification, using a 1x dilution of SSPE can help in the downstream applications, ensuring that the amplified fragments remain stable and intact during further analysis.

Where can I buy 20x SSPE Buffer 1 L in South Africa?

You can buy 20x SSPE Buffer 1 L from Ubuy. They offer a variety of laboratory supplies, including this specific buffer, making it a reliable choice for your research needs in South Africa.

How to Shop 20x SSPE Buffer 1 L Online From Ubuy?

It’s easy to shop 20x SSPE Buffer 1 L online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.

Is 20x SSPE Buffer 1 L Available to Shop Online in South Africa?

Yes, at Ubuy South Africa this product is available for you to shop at a reasonable price. The 20x SSPE Buffer 1 L is not available locally but you can trust us with our express shipping services.

How Long Does It Take to Get Product After Placing the Order?

The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

20X SSPE is a prepared buffer concentrate for nucleic acid hybridizations and blot transfer applications.

Item #: 125665119

20x SSPE Buffer 1 L

Item #: 125665119

ZAR 1212

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20X SSPE is a prepared buffer concentrate for nucleic acid hybridizations and blot transfer applications.

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What Stands Out

High Concentration

The 20x SSPE buffer provides a high concentration superlative for nucleic acid hybridization studies, allowing researchers to achieve optimal conditions for accurate results while minimizing the volume used in experiments.

Ready-to-Use

Each liter of the 20x SSPE buffer is pre-formulated, saving researchers time in preparation. This convenience ensures consistent quality and reliability across various applications in molecular biology research.

Versatile Application

Ideal for multiple applications, including DNA/RNA hybridization and blotting techniques, this buffer addresses diverse experimental needs, making it a valuable resource for laboratories focused on genetic research.

Product Details

Item Weight

2.2 lbs (1 kg)

Who Should Buy?

Molecular Biologists
Ideal for those conducting nucleic acid hybridizations or PCR, enhancing probe sensitivity and specificity in experiments.
Laboratory Technicians
Utilized for preparing buffers in various laboratory applications, ensuring consistent results across multiple experiments.
Research Institutions
Supports extensive research projects in genomics and proteomics, facilitating efficient data collection and analysis.

Casual Hobbyists
Not suitable for amateur experimenters due to the complexity and precision required in molecular biology applications.
Short-Term Use
Provides long-term stability but not ideal for single-use applications; larger volumes may go to waste.
Budget-Conscious Users
Higher cost compared to alternatives may deter individuals or small labs with limited funding from purchasing.

Product Description

20x SSPE Buffer 1 L

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Customer Questions & Answers

Question: How to Shop 20x SSPE Buffer 1 L Online From Ubuy?

Answer: It’s easy to shop 20x SSPE Buffer 1 L online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.
Question: Is 20x SSPE Buffer 1 L Available to Shop Online in South Africa?

Answer: Yes, at Ubuy South Africa this product is available for you to shop at a reasonable price. The 20x SSPE Buffer 1 L is not available locally but you can trust us with our express shipping services.
Question: How Long Does It Take to Get Product After Placing the Order?

Answer: The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

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Limitations : For products shipped internationally, please note that any manufacturer warranty may not be valid; manufacturer service options may not be available; product manuals, instructions, and safety warnings may not be in destination country languages; the products (and accompanying materials) may not be designed in accordance with destination country standards, specifications, and labeling requirements; and the products may not conform to destination country voltage and other electrical standards (requiring use of an adapter or converter if appropriate). The recipient is responsible for assuring that the product can be lawfully imported to the destination country. When ordering from Ubuy or its affiliates, the recipient is the importer of record and must comply with all laws and regulations of the destination country.
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ZAR 1212

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Features & Benefits

Ready-to-use 20X SSPE buffer concentrate for ease of use.
Ideal for nucleic acid hybridizations and blotting.
Liquid form ensures convenience in application.
Composed of essential components: NaCl, NaH2PO4, EDTA.
Stable at room temperature for easy storage and handling.
High concentration offering effective results in gel electrophoresis and blotting.

20x SSPE Buffer 1 L

ZAR 1212

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Free Return*

Secure Packaging

100% Original Products

PCI DSS Compliance

ISO 27001 Certified

What Stands Out

Product Details

Who Should Buy?

Product Description

Customer Questions & Answers

Buffers Editorial Review

Customer Reviews & Ratings

Review this product

Product Price History

Important information

ZAR 1212

Features & Benefits

Fast
Shipping

Free
Return^*