What is 10x PBS Buffer and what is it used for?

10x PBS (Phosphate-Buffered Saline) Buffer is a saline solution that is commonly used in biological research and laboratory settings. This buffer helps maintain a stable pH and osmotic balance in biological samples, making it essential for various applications like cell culture, protein purification, and immunoassays. The '10x' indicates it's a concentrated form, which can be diluted to 1x for use. It's important for maintaining cell health and facilitating many laboratory protocols.

How do you prepare 1x PBS from 10x PBS Buffer?

To prepare a 1x PBS from a 10x PBS Buffer, simply dilute it with distilled water. For every 1 part of 10x PBS, add 9 parts of distilled water. This results in a final solution that is isotonic and pH-stable, ideal for various biological applications. For example, if you need 100 mL of 1x PBS, mix 10 mL of 10x PBS with 90 mL of distilled water. Accurate dilution ensures that your experimental results are reliable.

What are the benefits of using PBS Buffer in cell culture?

PBS Buffer is widely used in cell culture as it provides a stable osmotic environment and maintains pH levels, critical for cell survival and function. It acts as a transport medium for cells during procedures such as washing, diluting, or transferring cells. Additionally, PBS contains salts that help in maintaining the ionic strength necessary for cellular activities. For cell biologists, using PBS ensures that their results related to cell behaviors are consistent and reproducible.

Is 10x PBS Buffer suitable for use in immunoassays?

Yes, 10x PBS Buffer is highly suitable for use in immunoassays, including ELISA and western blotting. Its composition helps to prevent non-specific binding of antibodies, thus enhancing the accuracy of the assay results. The buffer maintains a stable pH, which is crucial during the reaction steps. For researchers, using PBS ensures that their assays yield high sensitivity and specificity, leading to reliable data.

Can 10x PBS Buffer be used in molecular biology applications?

Absolutely! 10x PBS Buffer is widely utilized in molecular biology applications, such as DNA extraction, RNA handling, and PCR preparation. Its isotonic nature helps to protect nucleic acids from degradation while facilitating hybridization processes. In experiments where you're working with sensitive nucleic acids, using PBS can enhance the yield and integrity, ensuring optimal performance in downstream applications.

What storage conditions are recommended for 10x PBS Buffer?

10x PBS Buffer should be stored at room temperature in a cool, dry place, away from direct sunlight. When properly stored, it can remain stable for extended periods. For longer-term storage, some labs prefer to keep it refrigerated. Always ensure to label your buffer correctly and check for any precipitates before use. Proper storage helps maintain the integrity of the buffer, ensuring reliable results in your experiments.

What is the pH level of 10x PBS Buffer and why is it important?

The typical pH level of 10x PBS Buffer is around 7.4, which is physiologically relevant for most biological samples. This neutral pH is crucial for maintaining proper cellular functions and conducting various experiments without causing cellular stress. Buffers like PBS play a vital role in keeping the pH stable during reactions, especially in biological assays. Therefore, ensuring the right pH helps yield consistent and reproducible experimental results.

Can you use 10x PBS Buffer for protein assays?

Yes, 10x PBS Buffer is commonly used in protein assays such as western blots and protein quantification. Its role includes diluting samples and washing steps, where maintaining a suitable environment is crucial to preserve protein integrity and functionality. For example, buffers like PBS help minimize protein denaturation and non-specific binding, which can lead to more precise and accurate assay results.

How long can PBS Buffer be used after opening?

Once opened, 10x PBS Buffer should ideally be used within a few months to ensure its effectiveness. However, if stored correctly—in a tightly sealed container, protected from contamination, and under appropriate conditions—it can last longer. Always check for clarity and the absence of precipitates before use. Monitoring the buffer's condition ensures that your experimental procedures remain reliable and that your results are valid.

Where can I buy 10x PBS Buffer 4 L in South Africa?

You can purchase 10x PBS Buffer 4 L from Ubuy. They offer a wide range of laboratory supplies, including buffers commonly used for biological research. With Ubuy, you can find reliable products that are essential for your experiments, ensuring a smooth workflow in your laboratory settings.

How to Shop 10x PBS Buffer 4 L Online From Ubuy?

It’s easy to shop 10x PBS Buffer 4 L online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.

Is 10x PBS Buffer 4 L Available to Shop Online in South Africa?

Yes, at Ubuy South Africa this product is available for you to shop at a reasonable price. The 10x PBS Buffer 4 L is not available locally but you can trust us with our express shipping services.

How Long Does It Take to Get Product After Placing the Order?

The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

Each 10X PBS solution is ready to use upon dilution to the desired concentration.

Item #: 90789056

10x PBS Buffer 4 L

Item #: 90789056

ZAR 2643

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What Stands Out

High Concentration

The 10x PBS buffer offers a highly concentrated solution suitable for various laboratory applications, ensuring enhanced performance and reliability compared to standard formulations.

Large Volume

With 4 liters of buffer, this product is ideal for larger scale experiments, reducing the frequency of replenishment and saving time in busy lab environments.

Versatile Use

Designed for multiple applications including cell culture and protein work, this buffer meets diverse research needs, making it a valuable asset for any scientific project.

Product Details

Item Weight

8.8 lbs (3.99 kg)

Who Should Buy?

Laboratories
Ideal for research laboratories conducting experiments requiring consistent and reliable buffer solutions for biochemistry applications.
Cell Culture
Perfect for cell culture applications, providing the necessary osmotic balance and pH stability for growing cells.
Molecular Biology
Useful for molecular biology techniques, such as DNA/RNA extraction, where a buffer system is crucial for sample integrity.

General Consumers
Not suitable for general consumers with no background in scientific research or laboratory practices.
Non-Scientific Use
Not recommended for users needing buffers for non-research purposes, like cooking or household applications.
Small Scale Projects
May not be practical for small-scale experiments where lesser volumes of buffer are required or preferred.

Product Description

10x PBS Buffer 4 L

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Customer Questions & Answers

Question: How to Shop 10x PBS Buffer 4 L Online From Ubuy?

Answer: It’s easy to shop 10x PBS Buffer 4 L online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.
Question: Is 10x PBS Buffer 4 L Available to Shop Online in South Africa?

Answer: Yes, at Ubuy South Africa this product is available for you to shop at a reasonable price. The 10x PBS Buffer 4 L is not available locally but you can trust us with our express shipping services.
Question: How Long Does It Take to Get Product After Placing the Order?

Answer: The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

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Pros

Highly effective for experiments
Stable and long-lasting
Easy to use and handle
consistent results across tests
Suitable for various applications

Cons

Labeling could be clearer.

Product Price History

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Limitations : For products shipped internationally, please note that any manufacturer warranty may not be valid; manufacturer service options may not be available; product manuals, instructions, and safety warnings may not be in destination country languages; the products (and accompanying materials) may not be designed in accordance with destination country standards, specifications, and labeling requirements; and the products may not conform to destination country voltage and other electrical standards (requiring use of an adapter or converter if appropriate). The recipient is responsible for assuring that the product can be lawfully imported to the destination country. When ordering from Ubuy or its affiliates, the recipient is the importer of record and must comply with all laws and regulations of the destination country.
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Features & Benefits

Ready-to-use 10X PBS solution for convenience.
Easy dilution to achieve desired concentration.
Ideal for applications like Western blot and ELISA.
Maintains a stable pH of 7.4 ± 0.2.
Composed of ultrapure-grade phosphate buffers.
Filtered for quality and reliability.

10x PBS Buffer 4 L

ZAR 2643

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100% Original Products

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What Stands Out

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Product Description

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Buffers Editorial Review

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Product Price History

Important information

ZAR 2643

Features & Benefits

Fast
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