What is Proteinase K used for?

Proteinase K is an enzyme primarily used in molecular biology for protein digestion. It effectively breaks down proteins, which helps in purifying nucleic acids by removing contaminating proteins during DNA or RNA extraction processes. In research labs, it is crucial for experiments requiring high-quality nucleic acids, such as PCR or cloning procedures.

How should Proteinase K be stored?

Proteinase K should be stored at -20°C to maintain its stability and activity. Upon reconstitution, it is advisable to aliquot the enzyme into smaller portions to prevent repeated freeze-thaw cycles, which could lead to the loss of enzymatic activity. Proper storage ensures optimal performance in various applications, including DNA and RNA extraction.

What is the optimal activity temperature for Proteinase K?

The optimal temperature for Proteinase K activity is approximately 55-65°C. At this temperature, the enzyme exhibits maximum digestion of proteins, making it highly efficient for protocols requiring thorough protein degradation. Using it at this temperature is essential when processing samples for clean nucleic acid isolation.

Does Proteinase K work with all types of proteins?

Yes, Proteinase K is versatile and can act on a wide range of proteins, including those that are heavily glycosylated or complexed. Its broad specificity makes it invaluable in molecular biology applications, as it can effectively digest various proteins present in cell lysates, thus ensuring the purity of nucleic acids.

Can Proteinase K inactivate viruses?

While Proteinase K is primarily designed for protein digestion, it can also inactivate certain viruses during nucleic acid extraction processes. The enzyme works by degrading viral proteins, thereby assisting in the purification of nucleic acids, essential in virology research where intact nucleic acid is required for further analysis.

What concentration of Proteinase K is typically used in applications?

A typical concentration of Proteinase K used in nucleic acid extraction protocols is between 20 to 100 µg/mL, depending on the sample type and required thoroughness of protein digestion. This range ensures effective removal of contaminants while preserving the quality of the target nucleic acids for downstream applications.

Is Proteinase K suitable for use in diagnostic procedures?

Yes, Proteinase K is suitable for use in certain diagnostic procedures, particularly those involving nucleic acid amplification techniques such as PCR. By ensuring the removal of contaminating proteins, it helps maintain the integrity and reliability of the DNA or RNA being analyzed, which is critical in clinical diagnostics.

What are the safety precautions when handling Proteinase K?

When handling Proteinase K, it is essential to wear appropriate personal protective equipment, including gloves and lab coats, to avoid skin contact. It is also advisable to work in a well-ventilated area to minimize inhalation of any aerosols. Following proper laboratory safety protocols ensures a safe working environment while using the enzyme.

How does Proteinase K compare to other proteases?

Proteinase K has a unique ability to function in a wide pH range and retains activity in the presence of detergents and urea, which sets it apart from other proteases. This property makes it a preferred choice for molecular biology applications over other proteases, providing reliability in results across various applications.

Where can I buy Proteinase K, 100 Milligrams in South Africa?

You can purchase Proteinase K, 100 Milligrams from Ubuy in South Africa. Ubuy offers a convenient online shopping experience with a range of laboratory products, ensuring you can find the right proteinase for your molecular biology applications delivered right to your door.

How to Shop Proteinase K, 100 Milligrams Online From Ubuy?

It’s easy to shop Proteinase K, 100 Milligrams online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.

Is Proteinase K, 100 Milligrams Available to Shop Online in South Africa?

Yes, at Ubuy South Africa this product is available for you to shop at a reasonable price. The Proteinase K, 100 Milligrams is not available locally but you can trust us with our express shipping services.

How Long Does It Take to Get Product After Placing the Order?

The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

RPI

Serine protease for protolytic inactivation of nucleases during isolation of DNA and RNA.

RPI

Item #: 11154192

Proteinase K, 100 Milligrams

85% of respondents would recommend this to a friend

Item #: 11154192

ZAR 3895

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What Stands Out

High Purity

Proteinase K is produced to high purity standards, ensuring reliable performance in enzymatic reactions, which enhances the accuracy of your laboratory results and reduces potential contamination.

Versatile Applications

This enzyme is suitable for a variety of applications, including DNA and RNA extraction, making it an essential tool for molecular biology, diagnostics, and research.

Scalable Quantity

Packaged in 100 milligrams, it provides an ample supply for multiple experiments, allowing laboratories to perform extensive research without frequent reordering, ultimately saving time and costs.

Product Details

Container Type	Bottle
Item Form	Powder
Item Weight	9.07 g
Brand Name	RPI
Manufacturer	RPI, Corp.
Part Number	P50220-0.1
Brand	RPI
Package Information	Bottle

Who Should Buy?

Molecular Biologists
Ideal for researchers requiring protein degradation in DNA or RNA extraction protocols to enhance yield and purity.
Laboratory Technicians
Useful for technicians involved in clinical applications needing efficient tissue sample processing and protein analysis.
Geneticists
Recommended for genetic studies where enzyme usage is crucial for isolating nucleic acids without contamination.

Home Users
Not suitable for casual users without laboratory experience due to the complexity of properly handling the enzyme.

Product Description

Proteinase K, 100 Milligrams

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Question: How to Shop Proteinase K, 100 Milligrams Online From Ubuy?

Answer: It’s easy to shop Proteinase K, 100 Milligrams online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.
Question: Is Proteinase K, 100 Milligrams Available to Shop Online in South Africa?

Answer: Yes, at Ubuy South Africa this product is available for you to shop at a reasonable price. The Proteinase K, 100 Milligrams is not available locally but you can trust us with our express shipping services.
Question: How Long Does It Take to Get Product After Placing the Order?

Answer: The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

RPI Analytical Reagents Editorial Review

Proteinase K, 100 Milligrams is a high-purity enzyme powder formulated for molecular biology applications. Packaged in a convenient bottle, this product from RPI is designed to facilitate protein digestion and is essential in various laboratory processes. The powdered form allows for easy use and accurate measurement. As a reliable source for molecular research, it has garnered positive feedback for its effectiveness. Users appreciate its consistency and quality, making it a valuable addition to research labs focusing on protein studies or DNA extraction protocols.

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Pros

High-purity enzyme for molecular biology applications
Convenient powder form for easy measurement
Reliable source for protein digestion tasks
Positive user feedback on consistency
Effective in DNA extraction protocols

Cons

Might require careful handling in laboratory settings

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Features & Benefits

Specifically designed for research or manufacturing use.
Effectively inactivates nucleases, ensuring DNA and RNA integrity.
Essential for accurate isolation processes.
Quality assurance with CAS Number: 39450-01-6.
Not intended for food or drug applications.
Ideal for professionals seeking reliable protolytic solutions.

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Proteinase K, 100 Milligrams

ZAR 3895

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Product Details

Who Should Buy?

Product Description

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RPI Analytical Reagents Editorial Review

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