What is 50X TAE Buffer used for?

50X TAE Buffer is primarily used in molecular biology for the electrophoresis of nucleic acids. This buffer maintains a stable pH during the separation of DNA and RNA and provides essential ions that help in the migration of nucleic acids through gels. When diluted to 1X concentration, it is optimal for running agarose gels, making it crucial in applications such as gel electrophoresis and DNA fragment analysis.

How do I dilute 50X TAE Buffer for use?

To use 50X TAE Buffer, it needs to be diluted to 1X concentration before application. This is achieved by mixing 1 part of the 50X solution with 49 parts of distilled water. For example, if you need 100 mL of 1X TAE Buffer, combine 2 mL of 50X TAE Buffer with 98 mL of water. This dilution is vital for effective DNA separation and band visualization in gel electrophoresis.

What is the shelf life of 50X TAE Buffer?

The shelf life of 50X TAE Buffer varies depending on storage conditions, but generally, it can remain stable for extended periods if kept in a cool and dry place. To ensure optimal performance, always check for any turbidity, discoloration, or precipitation before use. Proper storage will help maintain its effectiveness for experiments that involve DNA manipulation and analysis, reducing the risk of experimental failure.

Is 50X TAE Buffer compatible with agarose gel electrophoresis?

Yes, 50X TAE Buffer is highly compatible with agarose gel electrophoresis. It provides the necessary ionic environment that stabilizes the DNA while separating it based on size. This buffer is preferred for applications requiring high resolution in DNA fragment size determination. When diluted appropriately to 1X, it ensures effective migration of DNA through the gel matrix, allowing for clear visualization of the bands.

Can I use 50X TAE Buffer for RNA electrophoresis?

Yes, 50X TAE Buffer can be used for RNA electrophoresis, but its use in RNA analysis should be approached with care. It is important to ensure that the RNA samples are not degraded, and the buffer is fresh. RNA is sensitive to degradation; therefore, using 50X TAE Buffer allows better resolution of RNA bands when diluted correctly, promoting accurate electrophoresis results.

What are the components of 50X TAE Buffer?

50X TAE Buffer consists of Tris base, acetic acid, and EDTA (ethylenediaminetetraacetic acid). Tris acts as a buffering agent to maintain pH, acetic acid provides the acidic environment, and EDTA chelates divalent metal ions, which can otherwise interfere with DNA and enzymes. The combination creates a stable buffer system essential for nucleic acid studies, providing researchers with optimal conditions for their experiments.

Is 50X TAE Buffer necessary for DNA gel extraction?

50X TAE Buffer is not strictly necessary for DNA gel extraction but is useful in maintaining the integrity of DNA post-electrophoresis. After running your gel, the buffer can help rehydrate the gel and provide a stable pH environment for further DNA processing. It can also be beneficial when preparing samples for downstream applications, allowing for better recovery of DNA fragments due to its compatibility with various procedures.

How should I store 50X TAE Buffer?

To preserve the stability of 50X TAE Buffer, it should be stored in a cool, dark place at room temperature or refrigerated. Make sure the container is sealed tightly to prevent contamination. Proper storage conditions help prevent degradation or changes in composition, ensuring reliable results during experiments involving nucleic acids, such as PCR preparation or gel electrophoresis.

What are some common applications for using 50X TAE Buffer?

50X TAE Buffer has several applications, primarily in molecular biology, including gel electrophoresis for DNA and RNA, restriction enzyme digestion, and PCR preparations. It helps facilitate the migration of nucleic acids through gels, aiding in the quantification and analysis of DNA fragments. Researchers often use it for cloning experiments and during the preparation of samples for sequencing, ensuring high-quality results.

Where can I buy 50X TAE Buffer, 100 mL?

You can buy 50X TAE Buffer, 100 mL on Ubuy in South Africa. Ubuy provides a convenient online shopping platform where you can find various laboratory supplies, including different concentrations of TAE Buffer, ensuring you get the right product for your research needs.

How to Shop 50X TAE Buffer, 100 mL Online From Ubuy?

It’s easy to shop 50X TAE Buffer, 100 mL online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.

Is 50X TAE Buffer, 100 mL Available to Shop Online in South Africa?

Yes, at Ubuy South Africa this product is available for you to shop at a reasonable price. The 50X TAE Buffer, 100 mL is not available locally but you can trust us with our express shipping services.

How Long Does It Take to Get Product After Placing the Order?

The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

eConLabSupply

50X TAE Buffer protects DNA during electrophoresis, ensuring your results are reliable and accurate.

eConLabSupply

Item #: 97964044

50X TAE Buffer, 100 mL

Item #: 97964044

ZAR 918

Price Details

Excluding Shipping & Custom charges ( Shipping and custom charges will be calculated on checkout )

^{*All items will import from US}

Availability : In stock

Imported from USA store

QTY:

Order now and get it around Wednesday, July 08

Secured transaction

Our Top Logistics Partners

50X TAE Buffer protects DNA during electrophoresis, ensuring your results are reliable and accurate.

U-Care Warranty:

None

Select a Plan

Fast
Shipping

Free
Return^*

Secure Packaging

100% Original Products

PCI DSS Compliance

ISO 27001 Certified

Note: Step Down Voltage Transformer required for using electronics products of US store (110-120). Recommended power converters Buy Now.

What Stands Out

Concentrated Solution

This 50X TAE buffer provides a highly concentrated formulation, ensuring ease of use and longer shelf life, making it ideal for high-throughput laboratories and educational institutions.

Versatile Applications

Designed for various molecular biology techniques, this buffer ensures optimal performance for DNA electrophoresis and other applications, effectively meeting diverse research needs in a single product.

Precise Composition

Formulated with high purity components, this TAE buffer guarantees reproducibility and reliability in experiments, catering to both novice and expert researchers who demand consistent results.

Product Details

Item Weight

0.25 lbs (110 grams)

Who Should Buy?

Molecular Biologists
Ideal for electrophoresis applications, aiding in the separation and analysis of nucleic acids in a lab environment.
Academic Researchers
Perfect for students and researchers conducting experiments that require buffer solutions in their molecular biology studies.
DNA & RNA Studies
Essential for protocols involving DNA and RNA handling, ensuring stability and integrity during electrophoresis experiments.

Clinical Laboratories
Not suitable for clinical environments where strict regulatory compliance and high precision are required in diagnostics.
Undergraduate Courses
Beginner students may find ease of use with simpler buffers; TAE may be too complex for introductory labs.
Non-Biological Users
Individuals not engaged in biological research or laboratory work would not need this specialized buffer solution.

Product Description

50X TAE Buffer, 100 mL

Have any Query? Chat with us

Customer Questions & Answers

Question: How to Shop 50X TAE Buffer, 100 mL Online From Ubuy?

Answer: It’s easy to shop 50X TAE Buffer, 100 mL online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.
Question: Is 50X TAE Buffer, 100 mL Available to Shop Online in South Africa?

Answer: Yes, at Ubuy South Africa this product is available for you to shop at a reasonable price. The 50X TAE Buffer, 100 mL is not available locally but you can trust us with our express shipping services.
Question: How Long Does It Take to Get Product After Placing the Order?

Answer: The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

eConLabSupply Buffers Editorial Review

No editorial reviews found

Customer Reviews & Ratings

5.0

1 customers ratings

5 Star

100%
4 Star

0%
3 Star

0%
2 Star

0%
1 Star

0%

Review this product

Share your thoughts with other customers

Pros

High purity formula
Convenient 100 mL size
Well-suited for various applications
Easy to store and use
Reliable performance

Cons

Cap can be slightly hard to open.

Product Price History

Important information

Limitations : For products shipped internationally, please note that any manufacturer warranty may not be valid; manufacturer service options may not be available; product manuals, instructions, and safety warnings may not be in destination country languages; the products (and accompanying materials) may not be designed in accordance with destination country standards, specifications, and labeling requirements; and the products may not conform to destination country voltage and other electrical standards (requiring use of an adapter or converter if appropriate). The recipient is responsible for assuring that the product can be lawfully imported to the destination country. When ordering from Ubuy or its affiliates, the recipient is the importer of record and must comply with all laws and regulations of the destination country.
Not all the products listed on Ubuy are for sale, as Ubuy is a global search engine. Products are subject to export/trade regulations.

ZAR 918

Order now and get it around Wednesday, July 08

QTY:

Secured transaction

Our Top Logistics Partners

Fastest cross-border delivery

Features & Benefits

Contains 2M Tris, 1M Acetic acid, and 0.05M EDTA for effective nucleic acid electrophoresis.
Inhibits nucleases to protect DNA integrity during experiments.
Powers efficient running of both genomic and supercoiled DNA.
Faster dsDNA run compared to TBE buffer, enhancing experiment efficiency.
Manufactured under strict quality control in an ISO certified facility.
Ideal for research purposes, compliant with Good Manufacturing Practices.

50X TAE Buffer, 100 mL

ZAR 918

Fast Shipping

Free Return*

Secure Packaging

100% Original Products

PCI DSS Compliance

ISO 27001 Certified

What Stands Out

Product Details

Who Should Buy?

Product Description

Customer Questions & Answers

eConLabSupply Buffers Editorial Review

Customer Reviews & Ratings

Review this product

Pros

Cons

Product Price History

Important information

ZAR 918

Features & Benefits

Fast
Shipping

Free
Return^*