What is DMEM High Glucose, Pyruvate used for?

DMEM High Glucose, Pyruvate is a nutrient-rich cell culture medium widely used in laboratories for growing various types of cells, particularly mammalian cells. Its high glucose content supports rapid cell proliferation, while pyruvate serves as an additional energy source to enhance metabolic activity. This makes it ideal for experiments requiring fast-growing cells like fibroblasts or stem cells, or for studies in cancer research and drug testing.

How do I prepare DMEM High Glucose, Pyruvate for use?

To prepare DMEM High Glucose, Pyruvate for cell culture use, simply thaw the medium and warm it to room temperature or 37°C. Prior to application, you may need to supplement it with serum or other additives specific to your cell type. It's important to ensure sterility during this process to prevent contamination, which can affect your results. This preparation is crucial for maintaining optimal cell growth and ensuring experimental integrity.

What types of cells can be cultured in DMEM High Glucose, Pyruvate?

DMEM High Glucose, Pyruvate is suitable for culturing a variety of cell lines, including epithelial, fibroblast, and neuronal cells. It is especially effective for fast-growing cell lines like HEK293 and NIH 3T3 cells. Researchers often choose this medium for studies in cell biology, toxicology, and regenerative medicine due to its versatility and ability to support diverse cellular functions.

Is DMEM High Glucose, Pyruvate suitable for 3D cell culture?

Yes, DMEM High Glucose, Pyruvate can be utilized for 3D cell culture applications. Many researchers find that combining this medium with specific scaffolding materials helps create optimal conditions for mimicking in vivo environments, thus enhancing cell interactions and promoting tissue-like structures. This makes it a valuable tool for developing organoids or testing drug responses in a more physiologically relevant setting.

How long can DMEM High Glucose, Pyruvate be stored?

DMEM High Glucose, Pyruvate should be stored at 2-8°C when unopened, and it can generally be kept for several months. Once opened, it is advisable to use the medium within a few weeks for optimal performance. It's essential to check for any signs of contamination or abnormal changes before use, as these may compromise experimental results or cell viability.

Can I use DMEM High Glucose, Pyruvate without serum?

While DMEM High Glucose, Pyruvate can be used without serum, the absence of serum may limit cell growth and function since serum provides essential growth factors and hormones. For optimal results, researchers often supplement it with Fetal Bovine Serum (FBS) or other serum alternatives to enhance cell viability and encourage more productive cellular behaviors, making it a more comprehensive medium for experimental applications.

What are the key components of DMEM High Glucose, Pyruvate?

The key components of DMEM High Glucose, Pyruvate include glucose, sodium pyruvate, amino acids, vitamins, and salts. The high glucose concentration supports energetic demands of proliferating cells, while sodium pyruvate assists in buffering and providing energy. These components create a balanced nutritional environment that facilitates optimal cell growth, making it popular among scientists for various biological research applications.

Is DMEM High Glucose, Pyruvate suitable for primary cell cultures?

DMEM High Glucose, Pyruvate can be appropriate for primary cell cultures, depending on the specific needs of the cells being cultured. It provides a robust nutritional foundation that supports various cell types. However, it is often beneficial to tailor medium conditions or supplements based on the specific requirements of the primary cells to enhance attachment and proliferation. Always consult protocol resources or literature for guidance.

How is DMEM High Glucose, Pyruvate different from standard DMEM?

The main difference between DMEM High Glucose, Pyruvate and standard DMEM lies in the glucose and pyruvate concentrations. High Glucose DMEM contains elevated levels of glucose, which supports rapidly proliferating cells better than standard DMEM, while added pyruvate provides an extra energy source. This makes it particularly advantageous for applications involving fast-growing cell lines or cells with high metabolic demands.

Where can I buy DMEM High Glucose, Pyruvate, 500 mL in South Africa?

You can buy DMEM High Glucose, Pyruvate, 500 mL from Ubuy, which offers a wide range of lab supplies and reagents, ensuring you find the correct product suited for your research needs. Ubuy provides a hassle-free shopping experience with options to view product details, customer reviews, and various payment methods.

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Yes, at Ubuy South Africa this product is available for you to shop at a reasonable price. The DMEM High Glucose, Pyruvate, 500 mL is not available locally but you can trust us with our express shipping services.

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The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

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Dulbecco's Modified Eagle Medium is a versatile medium suitable for culturing various types of cells. It supports the growth of primary fibroblasts, neurons, glial cells, and more.

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Item #: 33717705

DMEM High Glucose, Pyruvate, 500 mL

Item #: 33717705

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Dulbecco's Modified Eagle Medium is a versatile medium suitable for culturing various types of cells. It supports the growth of primary fibroblasts, neurons, glial cells, and more.

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What Stands Out

High Glucose Formula

Designed for optimal cell growth, this high glucose formulation supports various cell lines, ensuring reliable performance in experimental settings.

Pyruvate Inclusion

Contains sodium pyruvate, a key component that enhances cellular energy metabolism, promoting healthier and more vigorous cell cultures.

Convenient Volume

Supplied in a 500 mL bottle, this generous amount is ideal for both routine experiments and large-scale applications, minimizing waste and maximizing efficiency.

Product Details

Item Weight

1.1 lbs (500 grams)

Who Should Buy?

Cell Culture Labs
Ideal for laboratories engaged in cell culture work requiring high glucose and pyruvate supplementation for optimal cell growth.
Research Institutions
Beneficial for research projects involving mammalian cells that thrive in nutrient-rich media for experimental consistency.
Biotechnology Companies
Useful for biotechnological applications involving high-performance cell lines requiring specific media formulations for product development.

Plant Biotechnology
Not suitable for plant cell cultures that typically require different media formulations not based on DMEM ingredients.
Microbial Growth
Does not support microbial growth since its formulation is tailored for mammalian cells, not bacteria or fungi.
Low Glucose Needs
Not ideal for cell lines or experiments where low glucose concentration is necessary for effective growth or analysis.

Product Description

DMEM High Glucose, Pyruvate, 500 mL

About This Item

Introducing the DMEM High Glucose, Pyruvate, 500 mL - the ultimate solution for all your cell culture needs. Dulbecco's Modified Eagle Medium (DMEM) is a high-quality, ultrapure medium that provides the perfect environment for the growth and proliferation of various types of cells. Whether you're working with human, mouse, rat, monkey, hamster, chicken, or fish cells, this medium is designed to support their growth and ensure optimal results.

This DMEM medium, when supplemented with fetal bovine serum, becomes an ideal choice for the culture of primary fibroblasts, neurons, glial cells, human umbilical vein endothelial cells, and smooth muscle cells. It's the go-to medium for culturing popular cell lines like HeLa, 293, Cos-7, and PC-12. At Ubuy, we understand that every cell culture scenario is unique.

That's why we offer custom bulk DMEM formulations to cater to your specific culture conditions. Simply place an order under our custom products section, and we'll provide you with a tailored solution that meets your exact requirements. With its high glucose content and pyruvate enrichment, this DMEM combines the necessary nutrients to support optimal cell growth and functionality.

Each bottle contains 500 mL of this superior medium, allowing you to perform multiple experiments and cultures with ease. When it comes to DMEM for e-commerce, Ubuy is your one-stop-shop. We provide the best DMEM products for cell culture, ensuring that you have access to affordable, high-quality options.

Rest assured that our DMEM is formulated to precise standards, offering you a stable and consistent product for your research needs. Not only is this DMEM medium ideal for cell growth, but it's also well-suited for tissue engineering and biomedical applications. Its optimal nutrient levels ensure that your cells receive the necessary resources for their development and functionality.

Don't miss out on the opportunity to buy DMEM online. Ubuy is committed to providing you with the best products for your research endeavors. Trust in our high-quality DMEM supplies and unlock the potential of your cell culture experiments.

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Question: How to Shop DMEM High Glucose, Pyruvate, 500 mL Online From Ubuy?

Answer: It’s easy to shop DMEM High Glucose, Pyruvate, 500 mL online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.
Question: Is DMEM High Glucose, Pyruvate, 500 mL Available to Shop Online in South Africa?

Answer: Yes, at Ubuy South Africa this product is available for you to shop at a reasonable price. The DMEM High Glucose, Pyruvate, 500 mL is not available locally but you can trust us with our express shipping services.
Question: How Long Does It Take to Get Product After Placing the Order?

Answer: The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

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Pros

Versatile media
Supports cell growth
Contains nutrients

Cons

Limited stability

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DMEM High Glucose, Pyruvate, 500 mL

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Product Description

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