What is Western Blot Stripping Solution used for?

Western Blot Stripping Solution is designed to remove antibodies that are bound to proteins on Western blots. This process allows the same blot membrane to be probed with different antibodies without needing a new membrane. It's essential in experiments where researchers seek to analyze multiple proteins from the same sample, maximizing resources and efficiency by allowing reuse of the blots.

How do I properly use Western Blot Stripping Solution?

To use Western Blot Stripping Solution, incubate your membrane in the solution at a specified temperature and time, typically around room temperature for 15-30 minutes. Afterward, rinse the membrane with buffer and re-probe with the desired antibody. This process helps in stripping away the existing antibodies while maintaining the integrity of the protein bands, which is crucial for accurate quantitative results.

Is Western Blot Stripping Solution compatible with all types of antibodies?

Most Western Blot Stripping Solutions are compatible with a wide range of primary antibodies, including both polyclonal and monoclonal types. However, some particularly gentle or complex antibodies might require testing prior to usage. It’s recommended to refer to the manufacturer's specifications and test a small sample to ensure compatibility, optimizing your experimental conditions and ensuring reliable results.

What are the best practices for storing Western Blot Stripping Solution?

Store Western Blot Stripping Solution at 2-8°C in a tightly sealed container to maintain its effectiveness. Avoid repeated freeze-thaw cycles, as they can diminish the solution's performance. Keeping it away from direct sunlight and monitoring its expiration date can help ensure consistent and reliable use in your blots, contributing to more accurate scientific outcomes.

Can I reuse Western Blot Stripping Solution?

Reusing Western Blot Stripping Solution is not generally recommended because once it has been used, its effectiveness may be compromised. For best results, it's advisable to use fresh solution to ensure optimal stripping of antibodies and to avoid any potential cross-reactivity issues. This practice helps maintain the accuracy of follow-up experiments and data reproducibility.

What buffer should I use after stripping with Western Blot Stripping Solution?

After stripping, rinse the membrane thoroughly with a suitable wash buffer, commonly Phosphate Buffered Saline (PBS) or Tris Buffered Saline (TBS), to remove any residual stripping solution. Following this, you can proceed with the blocking step before probing with the new primary antibody. Using the correct buffer helps in preventing nonspecific binding and enhances the clarity of your Western blot results.

How long does it take to strip the membrane with Western Blot Stripping Solution?

The time required can vary, but typically, membranes can be stripped within 15 to 30 minutes when incubated at room temperature with the solution. However, this can also depend on the specific solution used and the type of antibodies present. Monitoring the stripping process helps in achieving optimal conditions and ensures that the original banding pattern is preserved for accurate re-probing.

Will stripping affect the quality of the Western blot?

When used correctly, Western Blot Stripping Solution does not adversely affect the quality of the Western blot. It effectively removes antibodies while preserving the integrity of protein bands. Improper use, however, may lead to loss of protein signals or distortion of bands. Therefore, adhering to the specified protocols ensures a high-quality outcome in your analyses, allowing for reliable interpretations.

What safety precautions should I take when using this solution?

When handling Western Blot Stripping Solution, it's important to wear appropriate personal protective equipment, including gloves, lab coats, and safety goggles. Ensure you're working in a well-ventilated area or fume hood, as some components may be irritants. By following these safety guidelines, you protect yourself from potential hazards during the research process and maintain a safe lab environment.

Where can I buy Western Blot Stripping Solution, 500ml in South Africa?

You can purchase Western Blot Stripping Solution, 500ml from Ubuy in South Africa. Ubuy offers a variety of laboratory supplies, including this specialized solution, ensuring you have access to high-quality products for your scientific research needs.

How to Shop Western Blot Stripping Solution, 500ml Online From Ubuy?

It’s easy to shop Western Blot Stripping Solution, 500ml online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.

Is Western Blot Stripping Solution, 500ml Available to Shop Online in South Africa?

Yes, at Ubuy South Africa this product is available for you to shop at a reasonable price. The Western Blot Stripping Solution, 500ml is not available locally but you can trust us with our express shipping services.

How Long Does It Take to Get Product After Placing the Order?

The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

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Item #: 80063112

Western Blot Stripping Solution, 500ml

Item #: 80063112

ZAR 2106

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What Stands Out

High Efficiency

This stripping solution offers rapid and effective removal of antibodies from blots, ensuring that your Western blotting experiments yield reliable results and reduce waste of valuable samples.

Versatile Application

Suitable for a wide range of antibodies and gel types, this solution allows researchers to re-probe blots confidently, maximizing the use of precious experimental materials without compromising integrity.

Large Quantity

Packaged in a convenient 500ml size, this solution provides incredible value for labs, allowing multiple stripping and probing cycles while minimizing the need for frequent reordering.

Product Details

Suitable for stripping membranes tested by ECL and other chemiluminescent reagents
Non-chemiluminescent reagents like DAB, NBT/BCIP are not suitable for western detection
Recommend conducting exploratory experiments for best elution conditions
Use nonfat dried milk solution for easier membrane regeneration
Best results attained with PVDF membrane
May form white precipitate at low temperature, which can be eliminated after water bath at 37℃

Item Weight

1.1 lbs (500 grams)

Who Should Buy?

Research Laboratories
Ideal for researchers needing to reuse Western blot membranes, offering efficient stripping and reprobing capabilities.
Protein Analysis
Suitable for scientists performing protein assays, ensuring accurate results by allowing multiple antibody applications on membranes.
Budget-Conscious Users
Cost-effective for labs looking to reduce waste and maximize resources by reusing membranes with efficient stripping solution.

Inexperienced Users
Not suitable for beginners unfamiliar with Western blot techniques, as improper usage can lead to significant errors.
Quantitative Studies
May not be ideal for studies requiring high precision, as residual antibodies can affect quantitative results.
Specific Antibody Types
Not recommended for fragile antibodies that may be degraded or affected by the stripping solution’s composition.

Product Description

Western Blot Stripping Solution, 500ml

About This Item

Introducing our Western Blot Stripping Solution, the ultimate solution for your Western blotting needs. With a generous volume of 500ml, this stripping solution is perfect for effectively removing antibodies from your blot, allowing you to reuse your membrane and save valuable time and resources. Our Western Blot Stripping Solution is specifically designed to provide efficient and reliable stripping of antibody-protein complexes, ensuring that your subsequent re-probing steps yield accurate and high-quality results. It effectively removes primary and secondary antibodies, without damaging the underlying proteins or compromising the integrity of your sample. Featuring a powerful formula, this stripping solution is suitable for a wide range of applications, making it an essential tool in any molecular biology or protein research laboratory.

Whether you are working with tissue samples, cell lysates, or purified proteins, our Western Blot Stripping Solution will deliver consistent and reproducible results. Not only does our stripping solution provide exceptional performance, but it is also incredibly easy to use. Simply apply the solution to your membrane and incubate. The powerful formulation will efficiently remove the bound antibodies, leaving your membrane ready for the next round of probing.

With this convenient and time-saving solution, you can streamline your Western blotting workflow and increase your productivity. When it comes to your research, quality and reliability are paramount. That's why our Western Blot Stripping Solution is manufactured to the highest industry standards, ensuring consistent performance and reproducible results with every use. We understand the importance of accuracy in your experiments and have formulated this solution to deliver exceptional stripping efficiency, ensuring that your subsequent analysis is precise and accurate. Achieve optimal results in your Western blotting experiments with our Western Blot Stripping Solution.

Order your 500ml bottle today and experience the convenience and reliability that this high-quality solution has to offer. Take your research to the next level with our trusted Western blotting products.

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Customer Questions & Answers

Question: How to Shop Western Blot Stripping Solution, 500ml Online From Ubuy?

Answer: It’s easy to shop Western Blot Stripping Solution, 500ml online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.
Question: Is Western Blot Stripping Solution, 500ml Available to Shop Online in South Africa?

Answer: Yes, at Ubuy South Africa this product is available for you to shop at a reasonable price. The Western Blot Stripping Solution, 500ml is not available locally but you can trust us with our express shipping services.
Question: How Long Does It Take to Get Product After Placing the Order?

Answer: The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

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Pros

Highly effective protein removal
Compatible with various membranes
Fast action typically under 30 minutes
Large 500ml volume for multiple uses
No significant background interference

Cons

Occasional odor during use

Product Price History

Important information

Limitations : For products shipped internationally, please note that any manufacturer warranty may not be valid; manufacturer service options may not be available; product manuals, instructions, and safety warnings may not be in destination country languages; the products (and accompanying materials) may not be designed in accordance with destination country standards, specifications, and labeling requirements; and the products may not conform to destination country voltage and other electrical standards (requiring use of an adapter or converter if appropriate). The recipient is responsible for assuring that the product can be lawfully imported to the destination country. When ordering from Ubuy or its affiliates, the recipient is the importer of record and must comply with all laws and regulations of the destination country.
Not all the products listed on Ubuy are for sale, as Ubuy is a global search engine. Products are subject to export/trade regulations.

ZAR 2106

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Features & Benefits

Removes primary and secondary antibodies from membranes during western blot experiments
Allows reuse of blots with other antibodies
Reduces variation in results
Suitable for membranes tested by ECL and other chemiluminescent reagents
Not suitable for western detection by non-chemiluminescent reagents
Recommended to conduct exploratory experiments to obtain best conditions

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Western Blot Stripping Solution, 500ml

ZAR 2106

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Secure Packaging

100% Original Products

PCI DSS Compliance

ISO 27001 Certified

What Stands Out

Product Details

Who Should Buy?

Product Description

About This Item

Customer Questions & Answers

Hygia Reagents Clinical Diagnostic Test Kits Editorial Review

Customer Reviews & Ratings

Review this product

Pros

Cons

Product Price History

Important information

ZAR 2106

Features & Benefits

Ubuy Assurance

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