What is 10X TAE and what is its primary use?

10X TAE (Tris Acetate EDTA) is a buffer solution widely used in molecular biology. It primarily serves to maintain a stable pH and ionic strength during techniques such as electrophoresis of nucleic acids. Its formulation allows researchers to separate DNA and RNA fragments effectively, ensuring integrity and visibility of the samples during analyses.

How is 10X TAE prepared for use in experiments?

To prepare 10X TAE for practical use, dilute it to a 1X solution with distilled water before use. This dilution allows for optimal buffering capacity during procedures like gel electrophoresis. It’s essential to mix thoroughly to ensure uniform concentration, which significantly enhances the performance of the electrophoresis process.

Why is the 0.22um filtering important for 10X TAE?

The 0.22um filtration process is crucial as it removes potential contaminants such as bacteria and particulates that could interfere with molecular biology experiments. Ensuring sterility helps protect sensitive reactions and enhances the integrity of experimental results, especially in applications like cloning and sequencing.

Can 10X TAE be stored, and if so, how?

Yes, 10X TAE can be stored for extended periods. To maintain its integrity, keep it in a cool, dark place, ideally refrigerated. Make sure the cap is tightly sealed to prevent any contamination or evaporation. This storage method preserves its buffering capacity and ensures reliable performance in future experiments.

Can I use 10X TAE for both DNA and RNA electrophoresis?

Absolutely, 10X TAE is suitable for electrophoresis of both DNA and RNA. Its buffering properties help maintain the integrity of nucleic acids during separation. This versatility makes it a preferred choice in many laboratories for various applications such as PCR, cloning, and RNA analysis.

What are the safety considerations when using 10X TAE?

When handling 10X TAE, it's essential to use personal protective equipment such as gloves and goggles to avoid skin and eye contact. Although this buffer is non-toxic, adhering to standard laboratory safety protocols ensures a safe working environment during experiments.

Is 10X TAE compatible with all types of gel electrophoresis?

Yes, 10X TAE is compatible with a variety of gel types, including agarose and polyacrylamide gels. Its ionic strength and pH stability enhance resolution during the separation of nucleic acids, making it suitable for various applications in biological research and education.

What is the difference between 10X TAE and 1X TAE?

10X TAE is a concentrated buffer solution that must be diluted to a 1X concentration for practical use in experiments. The primary difference lies in their usage, with 10X being utilized for storage and preparation, while 1X is employed in actual laboratory procedures, ensuring optimal conditions for nucleic acid analysis.

How does the pH of 10X TAE affect my experiments?

The pH of 10X TAE is typically around 8.0, which is essential for maintaining the stability of DNA and RNA during electrophoresis. A stable pH ensures that nucleic acids remain negatively charged, facilitating their movement through the gel matrix. Fluctuations in pH can lead to poor separation outcomes or degradation of samples.

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UFC Bio

10X TAE Tris Acetate EDTA - Sterile, 0.22um Filtered - 500 mL

UFC Bio

Item #: 84968611

10X TAE Tris Acetate EDTA - Sterile, 0.22um Filtered - 500 mL

Q: Where can I buy 10X TAE (Tris Acetate EDTA) - Sterile, 0.22um Filtered - 500 mL in South Africa?

You can purchase 10X TAE (Tris Acetate EDTA) - Sterile, 0.22um Filtered - 500 mL from Ubuy in South Africa. Ubuy offers an extensive selection of laboratory supplies and reagents, ensuring you get quality products that meet your scientific needs.

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What Stands Out

Sterile Solution

Ensures contamination-free experiments, providing reliable results in molecular biology applications.

0.22um Filtered

Removes unwanted particles effectively, maintaining high purity levels for sensitive analyses.

Generous Volume

500 mL capacity offers extensive use for repeated experiments, reducing the need for frequent replenishment.

Product Details

Item Weight

1.2 lbs (540 grams)

Who Should Buy?

Molecular Biologists
Ideal for molecular biology experiments, providing reliable buffer conditions for DNA and RNA electrophoresis.
Laboratory Technicians
Perfect for technicians needing a sterile, ready-to-use buffer for consistent results across multiple experiments.
Educational Institutions
Comprehensive solution for educational labs generating clear results in studies involving gel electrophoresis.

General Use
Not suitable for applications outside molecular biology, where other buffers may be more effective or affordable.
Non-sterile Applications
Should be avoided in procedures where non-sterile conditions are acceptable, as sterility is a key feature.
Short-term Storage
Not ideal for users who need a buffer for short-term use due to potential degradation of quality.

Product Description

10X TAE Tris Acetate EDTA - Sterile, 0.22um Filtered - 500 mL

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Question: How to Shop 10X TAE Tris Acetate EDTA - Sterile, 0.22um Filtered Online From Ubuy?

Answer: It’s easy to shop 10X TAE Tris Acetate EDTA - Sterile, 0.22um Filtered online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.
Question: Is 10X TAE Tris Acetate EDTA - Sterile, 0.22um Filtered Available to Shop Online in South Africa?

Answer: Yes, at Ubuy South Africa this product is available for you to shop at a reasonable price. The 10X TAE Tris Acetate EDTA - Sterile, 0.22um Filtered is not available locally but you can trust us with our express shipping services.
Question: How Long Does It Take to Get Product After Placing the Order?

Answer: The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

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10X TAE Tris Acetate EDTA - Sterile, 0.22um Filtered - 500 mL

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