What is Tris Base used for?

Tris Base is commonly utilized in biochemical and molecular biology laboratories as a buffering agent. Its ability to maintain a stable pH is critical during various experiments, including DNA/RNA electrophoresis, protein assays, and enzyme reactions. By providing a controlled environment, Tris helps ensure the reliability of experimental results, supporting precise analytical outcomes.

How should Tris Base be stored?

Tris Base should be stored in a cool, dry place away from direct sunlight to maintain its integrity. Proper storage is essential since exposure to moisture and heat can lead to degradation of the product. It’s advisable to keep it in its original container, tightly sealed, and labeled, to avoid contamination and ensure its efficacy for future experiments or applications.

What is the difference between Tris Base and other buffers?

Tris Base is distinguished from other buffering agents due to its effective pH range (typically 7.0 to 9.0) and low absorbance in UV light, making it ideal for biochemical applications. Unlike phosphates, which can participate in reactions, Tris maintains a stable environment without interfering with many biochemical processes. This makes it highly preferred in molecular biology settings for preparing solutions.

Can Tris Base be used in protein purification?

Yes, Tris Base is commonly used in protein purification techniques such as affinity chromatography and gel filtration. As a buffering agent, it helps to maintain pH stability, which is vital for preserving protein structure and function. Its role in controlling ionic strength also aids in optimizing separation processes during protein extraction and analysis.

Is Tris Base suitable for cell culture applications?

Tris Base can be used in specific cell culture applications, primarily as a buffer in various growth media. However, care should be taken as its pH-stabilizing properties may affect the media's composition. It’s often used in such contexts to ensure precise pH maintenance during experimental setups where consistent cellular environments are crucial for successful cell growth and experimentation.

What precautions should be taken when handling Tris Base?

When handling Tris Base, it is essential to wear appropriate personal protective equipment like gloves and goggles to avoid skin and eye irritation. It's also prudent to work in a well-ventilated area or fume hood. Proper lab safety protocols should be followed to prevent inhalation or accidental ingestion, ensuring a safe workspace while working with chemical reagents.

Can Tris Base be used in preparing electrophoresis buffers?

Absolutely! Tris Base is a key component in preparing both TAE and TBE buffers for electrophoresis. These buffers are crucial in maintaining a stable pH during the separation of nucleic acids. Its low conductivity allows for efficient migration of DNA/RNA, providing clarity in band visualization after gel electrophoresis, which is essential for successful molecular biology experiments.

What is the solubility of Tris Base in water?

Tris Base is highly soluble in water, typically dissolving readily at concentrations required for most laboratory applications. This high solubility ensures that solutions can be prepared with precision, making it an effective buffering agent in experiments. It is essential to prepare a concentrated stock solution and then dilute it as needed for specific tasks, optimizing performance in various biochemical assays.

How does Tris Base compare to PBS?

Tris Base and Phosphate-Buffered Saline (PBS) serve different purposes in laboratory applications. While PBS is used primarily for maintaining isotonic conditions for cells, Tris Base is favored for its buffering capacity over a wider pH range. Researchers often choose Tris when more stringent pH control is necessary or in cases where phosphate interference might be problematic, such as in certain enzymatic studies.

Where can I buy Tris Base (Ultrapure, 5 kg) in South Africa?

You can purchase Tris Base (Ultrapure, 5 kg) from Ubuy, which provides a reliable option for acquiring quality scientific products online. Simply visit the Ubuy website and search for Tris Base, where you'll find various options to choose from, making it easy to find the right product for your laboratory needs.

How to Shop Tris Base Ultrapure, 5 kg Online From Ubuy?

It’s easy to shop Tris Base Ultrapure, 5 kg online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.

Is Tris Base Ultrapure, 5 kg Available to Shop Online in South Africa?

Yes, at Ubuy South Africa this product is available for you to shop at a reasonable price. The Tris Base Ultrapure, 5 kg is not available locally but you can trust us with our express shipping services.

How Long Does It Take to Get Product After Placing the Order?

The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

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Experience the highest quality with our ULTRA PURE Tris base, ensuring reliability in your scientific endeavors.

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Item #: 180155808

Tris Base Ultrapure, 5 kg

Item #: 180155808

ZAR 6736

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What Stands Out

High Purity

This ultrapure Tris Base ensures minimal impurities, making it ideal for sensitive biological experiments and reliable buffer solutions in molecular biology applications.

Large Quantity

The 5 kg pack offers exceptional value, reducing the need for frequent reorders and ensuring consistent availability for laboratories with high usage rates.

Versatile Applications

Suitable for various applications in biochemistry and molecular biology, this Tris Base is ideal for buffer preparation, enhancing experimental flexibility for diverse research needs.

Product Details

Item Weight

11.02 lbs (5 kg)

Who Should Buy?

Laboratory Researchers
Ideal for researchers requiring high-purity Tris Base for biological and chemical experiments in a laboratory setting.
Molecular Biologists
Useful for molecular biologists working on buffers, electrophoresis, or DNA/RNA experiments needing reliable pH maintenance.
Academic Institutions
Perfect for universities and colleges offering courses in chemistry or biology that need dependable lab supplies.

Casual Users
Not suitable for individuals without laboratory skills or knowledge, as proper use requires scientific understanding.
Non-Scientific Use
Not recommended for applications outside of scientific research, as its purpose is strictly academic or laboratory-related.
Small Scale Projects
Purchasing 5 kg is excessive for hobbyists or small projects that require minimal quantities of buffering agents.

Product Description

Tris Base Ultrapure, 5 kg

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Customer Questions & Answers

Question: How to Shop Tris Base Ultrapure, 5 kg Online From Ubuy?

Answer: It’s easy to shop Tris Base Ultrapure, 5 kg online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.
Question: Is Tris Base Ultrapure, 5 kg Available to Shop Online in South Africa?

Answer: Yes, at Ubuy South Africa this product is available for you to shop at a reasonable price. The Tris Base Ultrapure, 5 kg is not available locally but you can trust us with our express shipping services.
Question: How Long Does It Take to Get Product After Placing the Order?

Answer: The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

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Pros

Good for biochemical applications
High purity level
Effective pH buffer
Reliable performance
Versatile usage

Cons

Requires careful handling.

Product Price History

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Limitations : For products shipped internationally, please note that any manufacturer warranty may not be valid; manufacturer service options may not be available; product manuals, instructions, and safety warnings may not be in destination country languages; the products (and accompanying materials) may not be designed in accordance with destination country standards, specifications, and labeling requirements; and the products may not conform to destination country voltage and other electrical standards (requiring use of an adapter or converter if appropriate). The recipient is responsible for assuring that the product can be lawfully imported to the destination country. When ordering from Ubuy or its affiliates, the recipient is the importer of record and must comply with all laws and regulations of the destination country.
Not all the products listed on Ubuy are for sale, as Ubuy is a global search engine. Products are subject to export/trade regulations.

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Features & Benefits

ULTRA PURE grade for maximum reliability.
Assay range of 99.9-101% for exceptional purity.
White crystalline appearance for visual assurance.
Molecular weight of 121.14 g/mol for precise calculations.
Convenient storage at room temperature.
Ideal for scientific and laboratory applications.

Tris Base Ultrapure, 5 kg

ZAR 6736

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Free Return*

Secure Packaging

100% Original Products

PCI DSS Compliance

ISO 27001 Certified

What Stands Out

Product Details

Who Should Buy?

Product Description

Customer Questions & Answers

Bioland Analytical Reagents Editorial Review

Customer Reviews & Ratings

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Pros

Cons

Product Price History

Important information

ZAR 6736

Features & Benefits

Fast
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