What is the PR2000-H-500 qMax Green, High Rox qPCR Mix?

The PR2000-H-500 qMax Green, High Rox qPCR Mix is a high-performance polymerase mix designed for quantitative polymerase chain reaction (qPCR) applications. This mix incorporates a unique green dye and high Rox reference dye to ensure optimal signal detection and enable quantification in various biological experiments. It's specifically formulated to enhance the efficiency of PCR, providing consistent and reliable results. Researchers often use this product in gene expression studies, pathogen detection, and genotyping applications.

What are the benefits of using High Rox qPCR Mix?

High Rox qPCR Mix offers several notable benefits, including improved assay sensitivity and reproducibility. The Rox dye serves as a passive reference, which normalizes signal fluctuations during PCR, leading to enhanced accuracy in quantifying nucleic acids. This is especially beneficial in multiplex assays, where different targets are analyzed in the same reaction. Users frequently report better results in experiments requiring precise quantification, such as studying low-abundance transcripts or exploring rare genetic variants.

How do you use the PR2000-H-500 qMax Green Mix in qPCR?

Using the PR2000-H-500 qMax Green Mix in qPCR involves a simple protocol. First, prepare your reaction mix by combining the qPCR mix with primers, probes, and template DNA. The recommended final concentration of the mix is generally around 1X, but always consult the specific protocol provided by your laboratory. Once mixed, transfer the reaction to a qPCR plate, seal it, and place it in a qPCR machine. This mix is versatile and can be applied in various experiments, from basic research to clinical diagnostics.

Is the PR2000-H-500 qMax Green Mix suitable for multiplex qPCR?

Yes, the PR2000-H-500 qMax Green Mix is suitable for multiplex qPCR applications. The high Rox stabilization allows for precise quantification even when multiple target sequences are amplified in the same reaction. Researchers commonly utilize multiplexing to conserve reagents and reduce sample handling time, making this mix an excellent choice for labs aiming to streamline their workflows. Additionally, users have found success in experiments where simultaneous detection of various pathogens is required.

Can I store the PR2000-H-500 qMax Green Mix? If so, how?

The PR2000-H-500 qMax Green Mix should be stored at -20°C for long-term storage to maintain its activity and effectiveness. It's important to avoid repeated freeze-thaw cycles as they can degrade the components over time. For optimal performance, aliquoting the mix into smaller portions can help preserve its integrity. This practice is particularly useful for laboratories that frequently use this mix, allowing them to thaw only what is needed for immediate use.

What types of samples can be analyzed using this qPCR mix?

The PR2000-H-500 qMax Green Mix is compatible with various sample types, including cDNA, genomic DNA, and RNA samples after reverse transcription. Whether you're working with clinical samples, environmental samples, or cultured cells, this mix can effectively amplify nucleic acid targets. It's versatile, making it ideal for applications ranging from pathogen detection to gene expression analysis in different research environments, enhancing the flexibility of your experimental design.

Are there any specific primers or probes recommended for use with this mix?

While there are no strict primer or probe recommendations exclusively for the PR2000-H-500 qMax Green Mix, it’s crucial to design them specifically for your target sequences considering factors such as annealing temperature and GC content. Optimized primers and probes enhance the efficiency of your qPCR reaction, and using this mix allows flexibility in designing your assays. Researchers often test various primer sets to achieve optimal results tailored to their specific experimental conditions.

What is the shelf life of PR2000-H-500 qMax Green Mix?

The shelf life of the PR2000-H-500 qMax Green Mix is typically indicated on the product packaging or data sheet. Generally, when stored properly at -20°C, users can expect a shelf life of several months to over a year without significant loss of performance. Always check the expiration date and perform control experiments to ensure the mix is still effective, especially if you have had it in storage for an extended period.

Can this qPCR mix be used in quantitative and qualitative assays?

Yes, the PR2000-H-500 qMax Green Mix can be utilized in both quantitative (qPCR) and qualitative PCR assays. In quantitative assays, the concentration of template DNA can be accurately measured, while in qualitative assays, the presence or absence of a specific target can be determined. This versatility makes it suitable for a broad range of applications, from basic research to diagnostic testing, providing researchers with a reliable tool for their molecular biology needs.

Where can I buy PR2000-H-500 qMax Green, High Rox qPCR Mix, 500 Reactions?

You can buy the PR2000-H-500 qMax Green, High Rox qPCR Mix from Ubuy. They offer this product online, ensuring that you're able to find it with ease. Ubuy provides a straightforward purchasing experience, making it simple for you to acquire this high-quality qPCR mix to support your research and experiments.

How to Shop Accuris PR2000-H-500 qMax Green, High Rox qPCR Mix, Online From Ubuy?

It’s easy to shop Accuris PR2000-H-500 qMax Green, High Rox qPCR Mix, online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.

Is Accuris PR2000-H-500 qMax Green, High Rox qPCR Mix, Available to Shop Online in South Africa?

Yes, at Ubuy South Africa this product is available for you to shop at a reasonable price. The Accuris PR2000-H-500 qMax Green, High Rox qPCR Mix, is not available locally but you can trust us with our express shipping services.

How Long Does It Take to Get Product After Placing the Order?

The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

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Accuris PR2000-H-500 qMax Green, High Rox qPCR Mix, 500 Reactions

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Item #: 161390882

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What Stands Out

High Performance

The PR2000-H-500 qMax Green offers exceptional sensitivity and specificity, ensuring reliable amplification of target DNA sequences, making it perfect for both research and clinical applications.

500 Reactions

Designed for efficiency, this mix provides up to 500 reactions, reducing costs and waste, making it an ideal choice for laboratories with high throughput needs.

User-Friendly

The mix is optimized for easy setup and consistent results, saving time in experimental workflows and allowing researchers to focus on their science with minimal troubleshooting.

Product Details

Button Quantity	3
Power Source	Battery Powered
Range	10.0 meters
Color	Green
Style Name	Modern
Brand	Accuris
Manufacturer	Accuris
Mfr Part Number	PR2000-H-500
Number of Buttons	3
Style	Modern
Item Weight	1 lbs (450 grams)

Who Should Buy?

Research Laboratories
Ideal for academic labs conducting gene expression studies requiring reliable and high-throughput qPCR performance.
Clinical Applications
Suitable for diagnostic labs needing precise quantification in various clinical research applications, ensuring reproducibility.
Environmental Testing
Effective for monitoring pathogens or contaminants in environmental samples, leveraging high sensitivity in diverse conditions.

Beginners
Not preferred for those new to qPCR, as it may require advanced knowledge for optimal use and setup.

Product Description

Accuris PR2000-H-500 qMax Green, High Rox qPCR Mix, 500 Reactions

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Question: How to Shop Accuris PR2000-H-500 qMax Green, High Rox qPCR Mix, Online From Ubuy?

Answer: It’s easy to shop Accuris PR2000-H-500 qMax Green, High Rox qPCR Mix, online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.
Question: Is Accuris PR2000-H-500 qMax Green, High Rox qPCR Mix, Available to Shop Online in South Africa?

Answer: Yes, at Ubuy South Africa this product is available for you to shop at a reasonable price. The Accuris PR2000-H-500 qMax Green, High Rox qPCR Mix, is not available locally but you can trust us with our express shipping services.
Question: How Long Does It Take to Get Product After Placing the Order?

Answer: The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

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Features & Benefits

Ready-to-use 2X master mix for convenience.
Engineered for high sensitivity and fast cycling.
Ideal for low copy number and difficult template sequences.
Early Ct values for quicker detection.
Includes Accuris Hot Start Taq Polymerase for accuracy.
Proprietary dye shows higher fluorescence and lower PCR inhibition.

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Accuris PR2000-H-500 qMax Green, High Rox qPCR Mix, 500 Reactions

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What Stands Out

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Accuris Natural Proteins Editorial Review

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Important information

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