What is Tris buffer, and why is it used?

Tris buffer, or tris(hydroxymethyl)aminomethane, is a widely used buffering agent in biological and chemical laboratories. It stabilizes the pH of solutions, particularly in molecular biology applications, such as DNA and RNA extraction, electrophoresis, and enzyme reactions. Having a stable pH is vital for the activity of many biomolecules and processes, making Tris buffer essential in experiments that require precision.

What is the pH and concentration of this Tris buffer?

The Tris buffer you are considering has a pH of 7.5 and a concentration of 1M. This specific pH is ideal for many biological applications, mimicking physiological conditions and ensuring optimum performance for enzymes and reactions. The 1M concentration allows for effective buffering capacity, making it suitable for various laboratory protocols where maintaining a consistent pH is crucial.

Can Tris buffer be used for DNA electrophoresis?

Yes, Tris buffer is commonly used in DNA electrophoresis. Its ability to maintain a stable pH during the separation of nucleic acids is critical for accurate and reproducible results. By using a Tris-based buffer, you help ensure that the DNA retains its integrity and migrates properly through the gel during electrophoresis, making it a go-to choice for genetic analysis.

Is Tris buffer safe to handle?

Tris buffer is generally considered safe to handle in laboratory settings. While it is not highly hazardous, it is essential to wear appropriate personal protective equipment, such as gloves and goggles, when working with any chemicals. Its low toxicity makes it user-friendly for various applications, provided standard laboratory safety protocols are followed to minimize any potential exposure.

How should Tris buffer be stored?

Tris buffer should be stored at room temperature away from direct sunlight. It is recommended to keep it in a tightly sealed container to prevent contamination and evaporation. Proper storage ensures the buffer retains its effectiveness for laboratory applications over time. Additionally, always check for any signs of precipitation or discoloration before use to ensure optimal performance.

What are common applications for Tris buffer?

Tris buffer is versatile and widely used in various applications, including molecular biology, biochemistry, and microbiology. Common uses include DNA/RNA extraction, protein electrophoresis, cell culture, and in assays requiring precise pH control. Its ability to maintain a stable pH under different conditions makes it ideal for experiments that involve enzymatic reactions or protein interactions.

Can Tris buffer be used in conjunction with other reagents?

Absolutely, Tris buffer is often used alongside other reagents in laboratory protocols. It is compatible with many common solutions such as agarose gels for DNA electrophoresis, various dye systems in assays, and enzyme substrates. When formulating experiments, researchers frequently combine Tris buffer with other agents to enhance stability and effectiveness in biochemical reactions.

What is the difference between Tris buffer and other buffers?

Tris buffer is primarily chosen for its buffering capacity around neutral pH, typically between 7 and 9, making it suitable for a range of biological processes. Unlike other buffers, such as phosphate buffers, Tris does not precipitate at high concentrations of divalent cations, which is advantageous in many biological experiments. Each buffer has its strengths; selecting the right one depends on the specific requirements of your experiment.

How is Tris buffer made?

Tris buffer can be easily prepared by dissolving tris base in distilled water and adjusting the pH to the desired level using hydrochloric acid (HCl). For a 1M solution at pH 7.5, specific quantities of tris base and HCl are calculated. This allows for customization of buffer concentration and pH to meet experimental needs. It is essential to verify pH with a pH meter for accuracy.

Where can I buy Tris Buffer, pH 7.5 [1M] - 1000 ml in South Africa?

You can purchase Tris Buffer, pH 7.5 [1M] - 1000 ml from Ubuy in South Africa. Ubuy offers a wide range of laboratory supplies and chemicals, ensuring that you can access high-quality products for your research and experiments. Explore the selection on Ubuy to find other useful laboratory items that may complement your work.

How to Shop Tris Buffer, pH 7.5 [1M] - 1000 ml, Online From Ubuy?

It’s easy to shop Tris Buffer, pH 7.5 [1M] - 1000 ml, online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.

Is Tris Buffer, pH 7.5 [1M] - 1000 ml, Available to Shop Online in South Africa?

Yes, at Ubuy South Africa this product is available for you to shop at a reasonable price. The Tris Buffer, pH 7.5 [1M] - 1000 ml, is not available locally but you can trust us with our express shipping services.

How Long Does It Take to Get Product After Placing the Order?

The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

eConLabSupply

Manufactured in an ISO 9001 and 13485 certified facility in the USA, our buffers are essential for protein research, molecular biology, and characterization.

eConLabSupply

Item #: 145280717

Tris Buffer, pH 7.5 [1M] - 1000 ml,

Item #: 145280717

ZAR 2112

Price Details

Excluding Shipping & Custom charges ( Shipping and custom charges will be calculated on checkout )

^{*All items will import from US}

Availability : In stock

Imported from USA store

QTY:

Order now and get it around Sunday, July 05

Secured transaction

Our Top Logistics Partners

Manufactured in an ISO 9001 and 13485 certified facility in the USA, our buffers are essential for protein research, molecular biology, and characterization.

U-Care Warranty:

None

Select a Plan

Fast
Shipping

Free
Return^*

Secure Packaging

100% Original Products

PCI DSS Compliance

ISO 27001 Certified

Note: Step Down Voltage Transformer required for using electronics products of US store (110-120). Recommended power converters Buy Now.

What Stands Out

Consistent pH

Maintains a stable pH level of 7.5, essential for reliable results in biochemical experiments and analyses.

High Purity

Formulated with high-quality reagents to ensure minimal contaminants, enhancing experimental accuracy and reproducibility.

Convenient Volume

Offered in a 1000 ml size, providing ample buffer for extensive experiments while allowing easy storage and use.

Product Details

Item Weight

2.2 lbs (1 kg)

Who Should Buy?

Molecular Biologists
Essential for DNA and RNA work, Tris buffer maintains stable pH during experiments and reactions.
Biochemical Researchers
Ideal for enzyme assays requiring precise pH; Tris buffer offers reliable performance in various biochemical reactions.
Laboratory Educators
Useful for teaching purposes in biological labs; demonstrates pH adjustment and buffer capacity in practical experiments.

Clinical Applications
Not suitable for medical use; Tris buffer lacks sterilization and specific regulations required for clinical settings.
Non-Aqueous Systems
Ineffective in non-aqueous environments; Tris buffer primarily function in aqueous solutions, limiting its versatility.
Extreme pH Conditions
Not appropriate for extreme pH settings; Tris buffer stability diminishes outside the optimal range of pH 7-9.

Product Description

Tris Buffer, pH 7.5 [1M] - 1000 ml,

Have any Query? Chat with us

Customer Questions & Answers

Question: How to Shop Tris Buffer, pH 7.5 [1M] - 1000 ml, Online From Ubuy?

Answer: It’s easy to shop Tris Buffer, pH 7.5 [1M] - 1000 ml, online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.
Question: Is Tris Buffer, pH 7.5 [1M] - 1000 ml, Available to Shop Online in South Africa?

Answer: Yes, at Ubuy South Africa this product is available for you to shop at a reasonable price. The Tris Buffer, pH 7.5 [1M] - 1000 ml, is not available locally but you can trust us with our express shipping services.
Question: How Long Does It Take to Get Product After Placing the Order?

Answer: The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

eConLabSupply Buffers Editorial Review

No editorial reviews found

Customer Reviews & Ratings

5.0

1 customers ratings

5 Star

100%
4 Star

0%
3 Star

0%
2 Star

0%
1 Star

0%

Review this product

Share your thoughts with other customers

Product Price History

Important information

Limitations : For products shipped internationally, please note that any manufacturer warranty may not be valid; manufacturer service options may not be available; product manuals, instructions, and safety warnings may not be in destination country languages; the products (and accompanying materials) may not be designed in accordance with destination country standards, specifications, and labeling requirements; and the products may not conform to destination country voltage and other electrical standards (requiring use of an adapter or converter if appropriate). The recipient is responsible for assuring that the product can be lawfully imported to the destination country. When ordering from Ubuy or its affiliates, the recipient is the importer of record and must comply with all laws and regulations of the destination country.
Not all the products listed on Ubuy are for sale, as Ubuy is a global search engine. Products are subject to export/trade regulations.

ZAR 2112

Order now and get it around Sunday, July 05

QTY:

Secured transaction

Our Top Logistics Partners

Fastest cross-border delivery

Features & Benefits

Comprehensive range of biological buffers for various applications.
Ideal for protein research, molecular biology, and diagnostics.
Produced in an ISO certified facility in the USA.
Each lot undergoes stringent Good Manufacturing Practices.
1M Tris Buffer pH 8.0 is ready-to-use and versatile.
Suitable for DNA and RNA applications with sterile packaging.

Tris Buffer, pH 7.5 [1M] - 1000 ml,

ZAR 2112

Fast Shipping

Free Return*

Secure Packaging

100% Original Products

PCI DSS Compliance

ISO 27001 Certified

What Stands Out

Product Details

Who Should Buy?

Product Description

Customer Questions & Answers

eConLabSupply Buffers Editorial Review

Customer Reviews & Ratings

Review this product

Product Price History

Important information

ZAR 2112

Features & Benefits

Fast
Shipping

Free
Return^*