What is Tris-HCl Buffer, pH 7.5 used for?

Tris-HCl Buffer, pH 7.5 is commonly used in biological and biochemical applications, particularly in DNA and RNA work. This buffer maintains a stable pH in various experiments, which is crucial for reactions such as PCR and enzyme assays. By providing a controlled environment for biomolecules, it ensures optimal conditions for enzymatic activity and stability.

Is Tris-HCl Buffer, pH 7.5 suitable for protein studies?

Yes, Tris-HCl Buffer, pH 7.5 is frequently utilized in protein studies. Its buffering capacity is ideal for maintaining the pH during protein purification and electrophoresis. This stability helps prevent denaturation of proteins, making it an essential component in many laboratory protocols focused on protein analysis.

Can I use Tris-HCl Buffer for electrophoresis?

Absolutely, Tris-HCl Buffer, pH 7.5 is widely used in gel electrophoresis, particularly for separating nucleic acids and proteins. The buffer's pH level optimizes the charge and behavior of biomolecules during the electrophoretic process, providing clearer separation and better resolution when visualizing the results.

What concentration is Tris-HCl Buffer, pH 7.5 provided in?

Tris-HCl Buffer is offered in a 1M concentration, which means it contains one mole of Tris base per liter of solution. This high concentration is beneficial for a variety of experimental applications, allowing for precise buffering capabilities without significant dilution, thereby supporting effective results in biochemical applications.

How do I prepare Tris-HCl Buffer from powder?

To prepare Tris-HCl Buffer, you would begin by dissolving Tris base in distilled water and adjusting the pH with hydrochloric acid. Typically, you will dissolve 121.14 grams of Tris in 800 ml of water, then add HCl to reach a pH of 7.5, and finally make up the volume to 1 liter. This method allows for tailored solutions based on specific experimental needs.

What is the role of pH in Tris-HCl Buffer?

The pH of a buffer is critical as it affects the activity of enzymes and the stability of nucleic acids. Tris-HCl Buffer at pH 7.5 is particularly effective for biological experiments, as many enzymes, such as DNA polymerases, operate optimally around this pH. This creates a conducive environment for biochemical reactions, promoting efficient results.

How long can I store Tris-HCl Buffer, pH 7.5?

Tris-HCl Buffer can typically be stored at room temperature away from direct sunlight for extended periods, up to several months, provided it is kept sterile and sealed. This longevity makes it a convenient buffer for repeated use in laboratory settings. Just ensure to check for any precipitate or color change before use.

Is Tris-HCl Buffer compatible with all biochemical applications?

While Tris-HCl Buffer is versatile, it is not suitable for all applications, particularly those involving very acidic or basic conditions. It may interfere with certain chemical reactions in organic chemistry settings. Always consider the specific requirements of your experiment to ensure compatibility and optimal results.

Can Tris-HCl Buffer be used in molecular biology experiments?

Yes, Tris-HCl Buffer, pH 7.5, is extensively utilized in molecular biology experiments. It is ideal for DNA and RNA work, such as in cloning, sequencing, and PCR. The buffer's stability at physiological pH allows for efficient handling and analysis of nucleic acids, greatly enhancing the quality of molecular biology research.

Where can I buy Tris-HCl Buffer, pH 7.5 [1M] - 1000 ml in South Africa?

You can purchase Tris-HCl Buffer, pH 7.5 [1M] - 1000 ml from Ubuy. They offer a variety of laboratory supplies, providing a convenient option for acquiring essential reagents like this buffer directly online.

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Is Tris-HCl Buffer, pH 7.5 [1M] - 1000 ml Available to Shop Online in South Africa?

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How Long Does It Take to Get Product After Placing the Order?

The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

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Tris-HCl Buffer, pH 7.5 [1M] - 1000 ml

Item #: 88027277

ZAR 1886

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What Stands Out

Precise pH Control

This Tris-HCl buffer provides a stable pH of 7.5, ensuring reliable results in biological and chemical experiments where precise pH conditions are crucial.

High Concentration

With a 1M concentration, this buffer allows for optimal performance in various applications, reducing the need for excessive dilutions and waste during experiments.

Generous Volume

The 1000 ml volume ensures ample supply for extensive use in laboratories, making it a practical choice for both small-scale and large-scale research projects.

Product Details

Item Weight

2.2 lbs (1 kg)

Who Should Buy?

Biochemical Research
Ideal for scientists conducting experiments requiring stable pH in biochemical assays and molecular biology applications.
Laboratory Use
Perfect for laboratory settings where precise pH control in sample processing is critical for consistency in results.
Educational Purposes
Useful for educational institutions teaching biochemistry, offering students hands-on experience with buffer solutions.

Field Applications
Not suitable for outdoor or field-based experiments where environmental pH conditions can fluctuate significantly.
Acidic Solutions
Incompatible for experiments requiring acidic conditions, as Tris-HCl buffers are alkaline around pH 7.5.
Non-Aqueous Solutions
Not recommended for experiments involving organic solvents, as Tris buffers are primarily designed for aqueous environments.

Product Description

Tris-HCl Buffer, pH 7.5 [1M] - 1000 ml

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Question: How to Shop Tris-HCl Buffer, pH 7.5 [1M] - 1000 ml Online From Ubuy?

Answer: It’s easy to shop Tris-HCl Buffer, pH 7.5 [1M] - 1000 ml online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.
Question: Is Tris-HCl Buffer, pH 7.5 [1M] - 1000 ml Available to Shop Online in South Africa?

Answer: Yes, at Ubuy South Africa this product is available for you to shop at a reasonable price. The Tris-HCl Buffer, pH 7.5 [1M] - 1000 ml is not available locally but you can trust us with our express shipping services.
Question: How Long Does It Take to Get Product After Placing the Order?

Answer: The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

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Pros

Stable pH for experiments
Easy to use
Versatile application
High-quality formulation
Well-reviewed by scientists

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Bottle design could be improved.

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Limitations : For products shipped internationally, please note that any manufacturer warranty may not be valid; manufacturer service options may not be available; product manuals, instructions, and safety warnings may not be in destination country languages; the products (and accompanying materials) may not be designed in accordance with destination country standards, specifications, and labeling requirements; and the products may not conform to destination country voltage and other electrical standards (requiring use of an adapter or converter if appropriate). The recipient is responsible for assuring that the product can be lawfully imported to the destination country. When ordering from Ubuy or its affiliates, the recipient is the importer of record and must comply with all laws and regulations of the destination country.
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Features & Benefits

Comprehensive range of biological buffers for research
Manufactured in ISO certified facility in the USA
Buffers are crucial for protein research and molecular biology
Produced under Good Manufacturing Practices and quality control tests
Tris-HCl buffer widely used in molecular biology work
Certificate of Analysis available for each production lot

Tris-HCl Buffer, pH 7.5 [1M] - 1000 ml

ZAR 1886

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Important information

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