What is Citrate Buffer, 1M, pH 6.0 used for?

Citrate Buffer, 1M, pH 6.0, is primarily utilized in biochemical applications, particularly in molecular biology and biochemistry. It acts as a stabilizing agent that maintains pH conditions during various experiments, including enzyme reactions and protein assays. This buffer is essential for ensuring consistent results in DNA and RNA studies, as many assays require a specific pH for optimal performance.

How do you prepare Citrate Buffer, 1M, pH 6.0?

To prepare Citrate Buffer, 1M, pH 6.0, mix 1M citric acid and sodium citrate at a ratio that achieves the desired pH level. Typically, this involves combining 0.1M citric acid and 0.1M sodium citrate in appropriate proportions and adjusting the pH using a pH meter. Proper preparation is crucial for obtaining accurate results in your experiments, particularly when testing acids or bases, or calibrating pH-sensitive equipment.

Can I use Citrate Buffer for cell culture applications?

Citrate Buffer, 1M, pH 6.0 is not typically used directly in cell culture applications, but it can be beneficial for specific experimental setups that require a stable pH environment. For example, researchers might use it for washing cells or preparing reagents where pH stabilization is crucial. Always consider the specific requirements of your cell line before application, as pH sensitivity varies across different cell types.

Is Citrate Buffer, 1M, pH 6.0 suitable for protein analyses?

Yes, Citrate Buffer, 1M, pH 6.0 is ideal for protein analyses, particularly in assays involving enzyme activity and protein stability. Many proteins function optimally at a pH around 6.0, making this buffer perfect for maintaining conditions that are conducive to accurate measurements. This buffer helps minimize fluctuations that could impact enzyme kinetics or structural integrity during analysis.

What are the storage requirements for Citrate Buffer, 1M, pH 6.0?

Citrate Buffer, 1M, pH 6.0 should be stored in a cool, dry place, away from direct sunlight, to maintain its stability. Ideally, it should be stored at room temperature, in a tightly sealed container to prevent contamination. Proper storage is essential because it ensures the buffer remains effective for its intended applications, which may involve metabolic or chemical reactions sensitive to environmental changes.

Can Citrate Buffer be used in clinical laboratories?

Yes, Citrate Buffer, 1M, pH 6.0 finds application in clinical laboratories, particularly in diagnostic assays where maintaining a specific pH is vital for accuracy. It is often used in coagulation studies and sample preparations where pH can affect the behavior of various biomolecules. Its reliable performance makes it a staple in routine laboratory practices.

What precautions should I take when using Citrate Buffer?

When using Citrate Buffer, 1M, pH 6.0, it is essential to wear appropriate PPE, including gloves and goggles, to protect against potential splashes. Additionally, ensure that your workspace is clean and free from contaminants that could affect experimental results. Practicing good laboratory hygiene is critical to maintaining the integrity of your experiments and avoiding cross-contamination.

Is Citrate Buffer compatible with other buffer systems?

Citrate Buffer, 1M, pH 6.0 can be compatible with certain other buffer systems depending on the specific requirements of your application. For example, it may be mixed with phosphate buffers in certain scenarios to achieve a desired pH or ionic strength. However, care must be taken, as combining buffers can lead to unpredictable results; it’s pivotal to test compatibility for your specific experimental conditions.

What is the role of pH in experiments using Citrate Buffer?

In experiments involving Citrate Buffer, pH plays a crucial role in influencing molecular interactions and enzyme activity. Many biochemical processes are pH-dependent; thus, maintaining a stable pH at 6.0 is essential for achieving reproducibility in experimental results. It's particularly relevant in enzyme assays where deviations from optimal pH can lead to altered reaction rates or product formation.

Where can I buy Citrate Buffer, 1M, pH 6.0, 250 mL in South Africa?

You can purchase Citrate Buffer, 1M, pH 6.0, 250 mL from Ubuy in South Africa. Ubuy provides a reliable platform for sourcing quality laboratory reagents, including this essential buffer. By shopping there, you can ensure that you are getting a product that meets high standards for your experimental and research needs.

How to Shop Citrate Buffer, 1M, pH 6.0, 250 mL Online From Ubuy?

It’s easy to shop Citrate Buffer, 1M, pH 6.0, 250 mL online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.

Is Citrate Buffer, 1M, pH 6.0, 250 mL Available to Shop Online in South Africa?

Yes, at Ubuy South Africa this product is available for you to shop at a reasonable price. The Citrate Buffer, 1M, pH 6.0, 250 mL is not available locally but you can trust us with our express shipping services.

How Long Does It Take to Get Product After Placing the Order?

The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

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Item #: 84061248

Citrate Buffer, 1M, pH 6.0, 250 mL

Item #: 84061248

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What Stands Out

Optimal pH Control

Ensures reliable results in experiments by maintaining a stable pH of 6.0, critical for biochemical and molecular applications.

High Concentration

This 1M concentration offers enhanced buffer capacity, effectively stabilizing pH during reactions and providing robust support for various laboratory applications.

Convenient Volume

With a 250 mL volume, this buffer provides ample supply for multiple experiments, reducing the need for frequent reordering and optimizing lab efficiency.

Product Details

Item Weight

0.55 lbs (250 grams)

Who Should Buy?

Biochemical Research
Ideal for laboratories conducting enzyme assays and other biochemical reactions requiring a stable pH environment.
Molecular Biology
Beneficial for molecular biologists needing a reliable buffer for DNA/RNA purification and manipulation.
Pharmaceutical Applications
Useful in the pharmaceutical industry for drug formulation and stability testing that requires specific pH conditions.

High-Temperature Experiments
Not suitable for experiments requiring high temperatures, as buffer stability may be compromised.
Basic pH Applications
Not ideal for applications requiring buffers with a pH above 7.0, limiting its use in alkaline processes.
Non-Aqueous Solutions
Inappropriate for use in non-aqueous solvent systems where citrate buffer will not function effectively.

Product Description

Citrate Buffer, 1M, pH 6.0, 250 mL

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Customer Questions & Answers

Question: How to Shop Citrate Buffer, 1M, pH 6.0, 250 mL Online From Ubuy?

Answer: It’s easy to shop Citrate Buffer, 1M, pH 6.0, 250 mL online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.
Question: Is Citrate Buffer, 1M, pH 6.0, 250 mL Available to Shop Online in South Africa?

Answer: Yes, at Ubuy South Africa this product is available for you to shop at a reasonable price. The Citrate Buffer, 1M, pH 6.0, 250 mL is not available locally but you can trust us with our express shipping services.
Question: How Long Does It Take to Get Product After Placing the Order?

Answer: The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

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Pros

Highly effective pH stabilizer
Easy to use and measure
Compatible with various applications
Reliable performance across experiments
Well-packaged for safe handling

Cons

Occasional sediment formation

Product Price History

Important information

Limitations : For products shipped internationally, please note that any manufacturer warranty may not be valid; manufacturer service options may not be available; product manuals, instructions, and safety warnings may not be in destination country languages; the products (and accompanying materials) may not be designed in accordance with destination country standards, specifications, and labeling requirements; and the products may not conform to destination country voltage and other electrical standards (requiring use of an adapter or converter if appropriate). The recipient is responsible for assuring that the product can be lawfully imported to the destination country. When ordering from Ubuy or its affiliates, the recipient is the importer of record and must comply with all laws and regulations of the destination country.
Not all the products listed on Ubuy are for sale, as Ubuy is a global search engine. Products are subject to export/trade regulations.

ZAR 1323

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Features & Benefits

Comprehensive range of biological buffers
Buffers important for protein research and molecular biology
Manufactured under stringent Good Manufacturing Practices
Citrate buffer's versatile applications in RNA isolation and immunofluorescent staining
Popular choice for biological research and clinical applications
For research use only. Not for human or animal clinical/therapeutic use

Citrate Buffer, 1M, pH 6.0, 250 mL

ZAR 1323

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Product Description

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Important information

ZAR 1323

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