What is a phosphate buffer and how is it used?

A phosphate buffer is a solution that maintains a stable pH level, specifically at pH 9.0 in this case. It is widely used in biological and biochemical applications, making it essential for experiments requiring a specific pH environment. By resisting changes in acidity or alkalinity, phosphate buffers are ideal for cell culture, enzyme reactions, and various laboratory procedures where pH stability is critical.

Why is a pH of 9.0 significant for phosphate buffers?

A pH of 9.0 is significant because it provides an alkaline environment suitable for various biochemical reactions. Many enzymes and proteins exhibit optimal activity at this pH, making phosphate buffers with this specific pH crucial in research and industry. For example, alkaline phosphatase, an enzyme used in molecular biology, thrives in such conditions, enhancing its functionality in experiments.

How should the phosphate buffer be stored?

Phosphate buffer should be stored in a cool, dry place, away from direct sunlight. Ideally, it should be kept in a tightly sealed, light-resistant container to prevent degradation. Proper storage is essential to maintain its effectiveness and stability, especially if you plan to use it across multiple experimental setups. For extended use, refrigerating can enhance longevity.

Can I use phosphate buffer in my laboratory experiments?

Yes, phosphate buffer is ideal for laboratory experiments, particularly those involving biochemical and biological assays. Its pH stability makes it suitable for cell cultures, DNA manipulation, and enzyme studies. When you're conducting experiments that require consistent environmental conditions, using a phosphate buffer helps ensure the reliability of your results while maximizing enzyme activity and reducing variability.

What are some common applications for phosphate buffers?

Phosphate buffers are commonly used in molecular biology, protein purification, and microbiological culture media. They are essential in applications like western blotting, enzyme assays, and electrophoresis, where maintaining a consistent pH is crucial. By providing a controlled environment, phosphate buffers enhance experimental accuracy and reproducibility, which are paramount in research settings.

Is the phosphate buffer toxic or hazardous?

The phosphate buffer at pH 9.0 is generally considered safe for use in laboratory settings. However, as with any chemical, it should be handled with care. Always utilize personal protective equipment (PPE) such as gloves and safety goggles, and ensure good ventilation. Familiarizing yourself with the material safety data sheet (MSDS) is advisable to understand any specific precautions.

What is the significance of the 0.5 M concentration?

The 0.5 M concentration indicates a moderate buffer capacity, meaning it can effectively resist changes in pH upon the addition of acids or bases. This concentration is particularly useful for experiments requiring reliable pH conditions without overpowering reactions. It allows flexibility in various applications while providing sufficient buffering to maintain a stable environment.

How can I prepare a phosphate buffer at pH 9.0?

To prepare a phosphate buffer at pH 9.0, you'll need to mix disodium hydrogen phosphate (Na2HPO4) and potassium dihydrogen phosphate (KH2PO4) in the correct proportions. By adjusting the amounts and measuring the pH, you can achieve your desired buffer solution. This method allows you to customize the buffer for specific experimental needs while ensuring it meets the pH requirements.

What is the shelf life of this phosphate buffer?

The shelf life of phosphate buffers typically ranges from several months to a year, depending on storage conditions. For optimal preservation, keep the buffer in a cool, dark place and avoid contamination. Always check for any visible signs of degradation before use. Proper storage practices can maintain its effectiveness for a longer duration, ensuring you have a reliable buffer when needed.

Where can I buy Phosphate Buffer, pH 9.0 [0.5 M] - 250 ml?

You can purchase Phosphate Buffer, pH 9.0 [0.5 M] - 250 ml on Ubuy in South Africa. Ubuy provides a convenient online shopping experience with a wide range of laboratory supplies, making it easy to find the specific buffer requirements for your experiments.

How to Shop Phosphate Buffer, pH 9.0 [0.5 M] - 250 ml Online From Ubuy?

It’s easy to shop Phosphate Buffer, pH 9.0 [0.5 M] - 250 ml online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.

Is Phosphate Buffer, pH 9.0 [0.5 M] - 250 ml Available to Shop Online in South Africa?

Yes, at Ubuy South Africa this product is available for you to shop at a reasonable price. The Phosphate Buffer, pH 9.0 [0.5 M] - 250 ml is not available locally but you can trust us with our express shipping services.

How Long Does It Take to Get Product After Placing the Order?

The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

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Phosphate Buffer, pH 9.0 [0.5 M] - 250 ml

Our biological buffers are essential for protein research, molecular biology, and diagnostics, manufactured in an ISO certified facility for guaranteed quality.

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Item #: 145283146

Phosphate Buffer, pH 9.0 [0.5 M] - 250 ml

Item #: 145283146

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What Stands Out

Pre-Prepared Solution

This phosphate buffer is ready to use, saving time and effort in preparation, ensuring consistency in experiments and reliability for researchers requiring a stable pH of 9.0.

High Concentration

With a 0.5 M concentration, this buffer provides superior buffering capacity, effectively maintaining pH levels during biochemical reactions, making it ideal for a variety of laboratory applications.

Versatile Applications

Suitable for various biochemical and molecular biology experiments, this buffer facilitates optimal conditions for reactions such as enzyme assays and protein purification, enhancing experimental outcomes.

Product Details

Item Weight

0.5 lbs (230 grams)

Who Should Buy?

Biochemical Research
Ideal for scientists conducting experiments requiring stable pH conditions in biochemical assays and reactions.
Molecular Biology
Useful for researchers performing DNA/RNA extractions, where maintaining pH is critical to ensure integrity.
Cell Culture
Beneficial for cell culture applications, particularly those requiring a stable pH for optimal cell growth and function.

Acidic Environments
Not suitable for applications requiring acidic conditions, as this buffer maintains a pH of 9.0.
Non-Biological Use
Inappropriate for industrial or non-scientific applications where buffering agents are not required.
Short Experiments
Not ideal for short-duration experiments where a simple buffer with less complexity is needed.

Product Description

Phosphate Buffer, pH 9.0 [0.5 M] - 250 ml

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Question: How to Shop Phosphate Buffer, pH 9.0 [0.5 M] - 250 ml Online From Ubuy?

Answer: It’s easy to shop Phosphate Buffer, pH 9.0 [0.5 M] - 250 ml online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.
Question: Is Phosphate Buffer, pH 9.0 [0.5 M] - 250 ml Available to Shop Online in South Africa?

Answer: Yes, at Ubuy South Africa this product is available for you to shop at a reasonable price. The Phosphate Buffer, pH 9.0 [0.5 M] - 250 ml is not available locally but you can trust us with our express shipping services.
Question: How Long Does It Take to Get Product After Placing the Order?

Answer: The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

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Limitations : For products shipped internationally, please note that any manufacturer warranty may not be valid; manufacturer service options may not be available; product manuals, instructions, and safety warnings may not be in destination country languages; the products (and accompanying materials) may not be designed in accordance with destination country standards, specifications, and labeling requirements; and the products may not conform to destination country voltage and other electrical standards (requiring use of an adapter or converter if appropriate). The recipient is responsible for assuring that the product can be lawfully imported to the destination country. When ordering from Ubuy or its affiliates, the recipient is the importer of record and must comply with all laws and regulations of the destination country.
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Features & Benefits

Comprehensive range of biological buffers for research and diagnostics.
Ideal for protein research, molecular biology, and characterization.
Manufactured in a USA facility certified to ISO 9001 and 13485.
Produced under stringent Good Manufacturing Practices.
Each batch undergoes rigorous quality control tests.
Popular for immunochemical staining and preserving living tissues.

Phosphate Buffer, pH 9.0 [0.5 M] - 250 ml

ZAR 1269

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