What is Citrate Buffer 0.1M used for?

Citrate Buffer 0.1M is primarily used in biochemical and molecular biology applications, including enzyme assays and electrophoresis. This buffer helps maintain a stable pH environment for various biological reactions, which is crucial for accurate experimental results. For example, researchers using this buffer can carry out protein electrophoresis without pH fluctuations that could affect the migration of proteins.

Why is it important to maintain a specific pH when using Citrate Buffer?

Maintaining a specific pH, especially in the range of 4.6 to 6.2 for Citrate Buffer, is critical for enzyme activity and stability. Many enzymes require a precise pH to function optimally, and deviations can lead to denaturation or inactivation. For scientists in enzymology, using this buffer ensures that enzymatic reactions occur under ideal conditions, providing reliable data and reproducibility in experiments.

How should Citrate Buffer 0.1M be stored?

Citrate Buffer 0.1M should be stored at room temperature in a tightly sealed container to prevent contamination. Avoid exposure to direct sunlight or extreme temperatures to maintain pH stability. For use in laboratory settings, proper storage ensures that the buffer retains its effectiveness for applications such as cell culture or molecular analyses, where pH consistency is paramount.

Can I use Citrate Buffer 0.1M for cell culture?

Yes, Citrate Buffer 0.1M can be used in specific cell culture applications, although it’s generally more common in biochemical experiments. It can help maintain appropriate pH levels when culturing sensitive cell lines. Researchers often rely on such buffers when performing assays where the pH must be strictly regulated to prevent cellular stress and ensure optimal cell growth during experiments.

What are the main components of Citrate Buffer?

Citrate Buffer is primarily composed of citric acid and its corresponding salts, such as sodium citrate. These components allow for effective buffering capacity within the specified pH range. The interplay between citric acid and its conjugate base stabilizes the pH during chemical reactions, making it a reliable choice for various laboratory applications where precise pH control is essential.

Is Citrate Buffer 0.1M suitable for PCR amplification?

Citrate Buffer 0.1M is generally not the first choice for PCR amplification, as PCR typically requires a more neutral pH for optimal enzyme function. However, it can be useful in specific pre-PCR preparations or certain protocols where maintaining a slightly acidic environment is necessary. Always refer to the specific protocol details or literature for best practices when using buffers in PCR.

What techniques can benefit from using Citrate Buffer?

Techniques such as gel electrophoresis, enzyme assays, and immunoassays can greatly benefit from using Citrate Buffer. The ability to maintain a consistent pH helps ensure that reactions proceed without interference. For example, in protein gel electrophoresis, using the correct buffer allows for accurate separation based on charge and size, leading to clearer results and more reliable analysis.

Can Citrate Buffer be prepared fresh, or is it better to buy it ready-made?

Both options are valid; Citrate Buffer can be prepared fresh based on specific concentrations and pH requirements, allowing for customization per protocol needs. However, purchasing ready-made Citrate Buffer ensures consistent quality and saves time in routine laboratory work. For researchers conducting multiple experiments, ready-made options streamline processes while maintaining reliability and reproducibility in results.

Is Citrate Buffer 0.1M compatible with other buffers?

Citrate Buffer 0.1M can be compatible with other buffers; however, mixing them should be approached with caution as it may lead to unexpected pH changes. Researchers often choose to use citrate as a standalone buffer to ensure optimal performance in enzymatic reactions or biochemical assays. It’s best suited to applications that specifically require its buffering properties without complicating the reaction conditions.

Where can I buy Citrate Buffer 0.1M, pH 4.6-6.2, 500 mL?

You can buy Citrate Buffer 0.1M, pH 4.6-6.2, 500 mL from Ubuy. Ubuy offers a wide range of laboratory supplies, ensuring that you have access to quality products for your scientific needs.

How to Shop Citrate Buffer 0.1M, pH 4.6-6.2, 500 mL Online From Ubuy?

It’s easy to shop Citrate Buffer 0.1M, pH 4.6-6.2, 500 mL online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.

Is Citrate Buffer 0.1M, pH 4.6-6.2, 500 mL Available to Shop Online in South Africa?

Yes, at Ubuy South Africa this product is available for you to shop at a reasonable price. The Citrate Buffer 0.1M, pH 4.6-6.2, 500 mL is not available locally but you can trust us with our express shipping services.

How Long Does It Take to Get Product After Placing the Order?

The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

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We offer a comprehensive range of biological buffers for use in research, diagnostics, drug development, and manufacturing.

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Item #: 79978951

Citrate Buffer 0.1M, pH 4.6-6.2, 500 mL

Item #: 79978951

ZAR 709

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What Stands Out

Precise pH Range

Maintains a stable pH level between 4.6 and 6.2, ensuring accurate results in various laboratory experiments and applications.

Large Volume

Supply of 500 mL is ideal for extended usage in multiple experiments, reducing the need for frequent reordering and boosting efficiency.

High Purity

Formulated with high-grade chemicals, guaranteeing minimal contaminants for reliable and reproducible results in sensitive scientific research.

Product Details

Manufactured in the USA in an ISO 9001 and 13485 certified facility
For research use only, not for human or animal clinical/therapeutic use
Each production lot undergoes stringent quality control tests under certified clean room conditions
Citrate buffer is ideal for RNA isolation and antigen detection
Popular choice for immunofluorescent staining applications in biological research and clinical pathology
Comprehensive range of biological buffers available for research, diagnostics, drug development, and manufacturing

Item Weight

1.1 lbs (500 grams)

Who Should Buy?

Laboratory Scientists
Ideal for scientists conducting experiments requiring stable pH levels in biochemical and molecular biology applications.
Educational Institutions
Useful for schools and universities teaching laboratory techniques in chemistry and biology involving pH buffers.
Pharmaceutical Research
Essential for researchers working on drug formulations where precise pH maintenance is crucial for stability and efficacy.

General Home Use
Not suitable for household applications, as it is a specialized laboratory chemical not typically needed at home.
Food Industry
Inappropriate for food applications due to potential regulatory and safety issues regarding chemical additives in food products.
Non-Scientific Users
Individuals without scientific background may misinterpret or misuse this buffer, leading to inaccurate results and safety concerns.

Product Description

Citrate Buffer 0.1M, pH 4.6-6.2, 500 mL

About This Item

Looking for a reliable citrate buffer for your biochemical applications? Look no further than our 0.1M Citrate Buffer, offering a pH range of 4.6-6.2. With a generous volume of 500 mL, this high-quality citrate buffer is perfect for your lab needs. Whether you are conducting research or carrying out experiments, our citrate buffer ensures consistent and accurate results.

Buy online now and experience the best in citrate buffering solutions.

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Customer Questions & Answers

Question: How to Shop Citrate Buffer 0.1M, pH 4.6-6.2, 500 mL Online From Ubuy?

Answer: It’s easy to shop Citrate Buffer 0.1M, pH 4.6-6.2, 500 mL online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.
Question: Is Citrate Buffer 0.1M, pH 4.6-6.2, 500 mL Available to Shop Online in South Africa?

Answer: Yes, at Ubuy South Africa this product is available for you to shop at a reasonable price. The Citrate Buffer 0.1M, pH 4.6-6.2, 500 mL is not available locally but you can trust us with our express shipping services.
Question: How Long Does It Take to Get Product After Placing the Order?

Answer: The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

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Pros

Accurate pH range (4.6-6.2)
Convenient 500 mL size
Excellent for biochemical applications
Stable formulation and long shelf life
Compatible with various assays

Cons

Limited use outside of pH range

Product Price History

Important information

Limitations : For products shipped internationally, please note that any manufacturer warranty may not be valid; manufacturer service options may not be available; product manuals, instructions, and safety warnings may not be in destination country languages; the products (and accompanying materials) may not be designed in accordance with destination country standards, specifications, and labeling requirements; and the products may not conform to destination country voltage and other electrical standards (requiring use of an adapter or converter if appropriate). The recipient is responsible for assuring that the product can be lawfully imported to the destination country. When ordering from Ubuy or its affiliates, the recipient is the importer of record and must comply with all laws and regulations of the destination country.
Not all the products listed on Ubuy are for sale, as Ubuy is a global search engine. Products are subject to export/trade regulations.

ZAR 709

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QTY:

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Fastest cross-border delivery

Features & Benefits

Comprehensive range of biological buffers
Suitable for research, diagnostics, drug development, and manufacturing
Manufactured in an ISO 9001 and 13485 certified facility
Each production lot manufactured under stringent quality control tests
Citrate buffers prevent base hydrolysis and break cross-links between antigens
Popular choice for immunofluorescent staining applications

Citrate Buffer 0.1M, pH 4.6-6.2, 500 mL

ZAR 709

Fast Shipping

Free Return*

Secure Packaging

100% Original Products

PCI DSS Compliance

ISO 27001 Certified

What Stands Out

Product Details

Who Should Buy?

Product Description

About This Item

Customer Questions & Answers

eConLabSupply Buffers Editorial Review

Customer Reviews & Ratings

Review this product

Pros

Cons

Product Price History

Important information

ZAR 709

Features & Benefits

Fast
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Free
Return^*