What is Triton X-100 Lysis Buffer, and what is it used for?

Triton X-100 Lysis Buffer is a widely used solution in biochemistry and molecular biology for cell lysis and protein extraction. This 2X buffer, at a pH of 7.4, facilitates the disruption of cell membranes, allowing the release of intracellular proteins and other biomolecules. It is particularly effective for lysing mammalian cells in research applications, making it ideal for preparing samples for assays, Western blots, and other analytical techniques.

How should Triton X-100 Lysis Buffer be prepared for use?

To prepare Triton X-100 Lysis Buffer for use, you should dilute the 2X stock solution with an appropriate buffer or water, following your specific experimental requirements. It’s essential to ensure the final concentration of Triton X-100 meets your lysis needs while maintaining the desired pH of 7.4. Typically, this buffer is used in a ratio that varies between experiments, so consulting relevant protocols can help you achieve optimal results.

Can Triton X-100 Lysis Buffer be used for all cell types?

While Triton X-100 Lysis Buffer is effective for a variety of cell types, its efficacy can differ. It is particularly suited for mammalian cells due to its ability to disrupt lipid membranes. However, for particular applications or cell types (like bacterial or plant cells), using alternative lysis buffers or adjusting the concentration may yield better results. Always refer to specific protocols related to the cell line or organism you are working with for best practices.

Is Triton X-100 Lysis Buffer safe to handle?

Triton X-100 is classified as a mild detergent, which is generally safe for laboratory handling. However, it can be irritating to skin and eyes, so it's advisable to wear appropriate personal protective equipment, such as gloves and safety goggles, while working with this buffer. Following standard laboratory safety procedures and ensuring good ventilation can help mitigate any potential risks associated with its use.

What conditions should Triton X-100 Lysis Buffer be stored under?

Triton X-100 Lysis Buffer should be stored at room temperature, away from direct sunlight to maintain its stability and effectiveness. It's important to keep the buffer sealed tightly to prevent contamination. Long-term storage in a refrigerator is not recommended as it may affect the properties of the buffer. Always check the buffer before use for any changes in clarity or precipitate.

Can I freeze Triton X-100 Lysis Buffer for long-term storage?

Freezing Triton X-100 Lysis Buffer is not generally recommended, as it can lead to changes in the buffer’s properties and efficacy. The buffer is best maintained at room temperature to ensure it remains effective for its intended purpose. If longer storage is necessary, consider aliquoting smaller amounts that can be used individually, reducing exposure to light and contamination.

What volume of Triton X-100 Lysis Buffer should I use for cell lysis?

The volume of Triton X-100 Lysis Buffer needed can depend on the number of cells being lysed. Typically, a range of 100 to 300 µL of buffer per 1 million cells is used. This allows for adequate lysis while ensuring that proteins are effectively extracted. It's advisable to optimize the volume based on preliminary experiments to ensure maximum yield and efficiency for your specific cellular context.

How does Triton X-100 Lysis Buffer compare to other lysis buffers?

Triton X-100 Lysis Buffer is favored for its effective cell membrane disruption and compatibility with a wide range of downstream applications. Compared to harsher lysis buffers, Triton X-100 is milder, preserving protein functionality, making it suitable for sensitive assays. Understanding the specific needs of your experimental setup will help in choosing the right lysis buffer, whether it be Triton X-100 or another type.

What are some practical applications for using Triton X-100 Lysis Buffer?

Triton X-100 Lysis Buffer is widely used in applications such as protein extraction for Western blot analysis, enzyme activity assays, and immunoprecipitation. Its ability to solubilize proteins while maintaining their functional integrity makes it a versatile choice. Researchers often use this buffer in cell biology studies to analyze signaling pathways, investigate protein interactions, or prepare samples for mass spectrometry.

Where can I buy Triton X-100 Lysis Buffer (2X), 7.4 pH, 500mL in South Africa?

You can buy Triton X-100 Lysis Buffer (2X), 7.4 pH, 500mL from Ubuy in South Africa. Ubuy offers a reliable platform for acquiring laboratory supplies, ensuring you get the necessary products for your research needs. Their inventory includes various sizes and formulations, helping you to find the exact buffer you require for your experiments.

How to Shop Triton X-100 Lysis Buffer (2X), 7.4 pH, 500mL Online From Ubuy?

It’s easy to shop Triton X-100 Lysis Buffer (2X), 7.4 pH, 500mL online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.

Is Triton X-100 Lysis Buffer (2X), 7.4 pH, 500mL Available to Shop Online in South Africa?

Yes, at Ubuy South Africa this product is available for you to shop at a reasonable price. The Triton X-100 Lysis Buffer (2X), 7.4 pH, 500mL is not available locally but you can trust us with our express shipping services.

How Long Does It Take to Get Product After Placing the Order?

The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

eCon Lab Supply

Triton X-100 Lysis Buffer (2X), 7.4 pH, 500mL

Manufactured in an ISO 9001 and 13485 certified facility in the USA, our biological buffers are essential for reliable protein research and molecular biology studies.

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Item #: 133308822

Triton X-100 Lysis Buffer (2X), 7.4 pH, 500mL

Item #: 133308822

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What Stands Out

Dual Concentration

The 2X formulation ensures efficient cell lysis while reducing the volume needed, making experiments more effective and saving valuable reagents.

Optimal pH Level

Maintaining a pH of 7.4 is ideal for preserving protein structure and function, optimizing extraction processes in biological research.

Generous Volume

With 500mL per bottle, researchers can perform multiple experiments without frequent repurchasing, ensuring cost-efficiency and convenience in laboratory settings.

Product Details

Container Type	Bottle
Item Form	Liquid
Item Weight	0.75 Pounds (340 grams)
Volume	500 Milliliters
Brand Name	eCon Lab Supply
Manufacturer	Ciro Mfg
Part Number	23.105.118
Included Components	Bottle
Brand	eCon Lab Supply
Item Volume	500 Milliliters
Package Information	Bottle

Who Should Buy?

Biochemists
Ideal for biochemical applications requiring cell lysis and protein extraction in various experimental setups.
Cell Biologists
Great for studying cellular components by facilitating the extraction of proteins and nucleic acids from cells.
Laboratory Researchers
Useful in various research laboratories focused on molecular biology and protein assays requiring lysis buffers.

Non-Scientific Users
Not suitable for casual users or individuals without proper laboratory training or equipment for safe usage.

Product Description

Triton X-100 Lysis Buffer (2X), 7.4 pH, 500mL

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Question: How to Shop Triton X-100 Lysis Buffer (2X), 7.4 pH, 500mL Online From Ubuy?

Answer: It’s easy to shop Triton X-100 Lysis Buffer (2X), 7.4 pH, 500mL online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.
Question: Is Triton X-100 Lysis Buffer (2X), 7.4 pH, 500mL Available to Shop Online in South Africa?

Answer: Yes, at Ubuy South Africa this product is available for you to shop at a reasonable price. The Triton X-100 Lysis Buffer (2X), 7.4 pH, 500mL is not available locally but you can trust us with our express shipping services.
Question: How Long Does It Take to Get Product After Placing the Order?

Answer: The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

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Limitations : For products shipped internationally, please note that any manufacturer warranty may not be valid; manufacturer service options may not be available; product manuals, instructions, and safety warnings may not be in destination country languages; the products (and accompanying materials) may not be designed in accordance with destination country standards, specifications, and labeling requirements; and the products may not conform to destination country voltage and other electrical standards (requiring use of an adapter or converter if appropriate). The recipient is responsible for assuring that the product can be lawfully imported to the destination country. When ordering from Ubuy or its affiliates, the recipient is the importer of record and must comply with all laws and regulations of the destination country.
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Features & Benefits

Comprehensive range of biological buffers for research and diagnostics.
Manufactured in the USA under ISO 9001 and 13485 certifications.
Critical for protein research, molecular biology, and characterization.
Produced in a certified 14644 cleanroom environment.
Triton X-100 widely used for cell lysis and membrane permeabilization.
Suitable for laboratories; not for clinical or therapeutic use.

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Triton X-100 Lysis Buffer (2X), 7.4 pH, 500mL

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