What is 10X Phosphate Buffered Saline (PBS)?

10X Phosphate Buffered Saline (PBS) is a concentrated saline solution used in biological and biochemical applications. It helps maintain the pH and osmotic balance, crucial for maintaining cell viability and function. PBS is often diluted to 1X for experiments, making it versatile in lab settings. It's commonly used in cell culture, molecular biology, and immunology applications, providing a stable environment for various biological assays.

How do I prepare 1X PBS from 10X PBS?

To prepare 1X PBS from 10X PBS, mix one part of 10X PBS with nine parts of distilled water. For instance, if you need 100 mL of 1X PBS, mix 10 mL of 10X PBS with 90 mL of distilled water. This dilution method ensures you maintain the correct phosphate and sodium concentrations necessary for experiments, ensuring optimal conditions for cell culture and enzymatic reactions.

What applications can benefit from 10X PBS?

10X PBS is widely used in laboratory settings for applications such as cell culture, buffer solutions in biochemical assays, dilution of proteins, and washing tissues for immunohistochemical staining. Its ability to maintain pH stability and provide an isotonic environment makes it ideal for cell viability assays and various molecular biology techniques.

Is 10X PBS safe to handle?

While 10X PBS is generally safe to handle, basic lab safety should be adhered to, such as wearing gloves and goggles. It contains salts and phosphates, which are not typically hazardous but can irritate eyes and skin. Always follow your institution's safety protocols and dispose of any waste according to local regulations.

Can 10X PBS be stored long-term?

Yes, 10X PBS can be stored long-term if kept sealed and refrigerated. Ensure that the container is tightly closed to prevent contamination and evaporation. Storing it at 2-8°C helps maintain its integrity. Always check for any changes in appearance before use, and if unsure, prepare fresh PBS.

What pH range does 10X PBS typically maintain?

10X PBS typically maintains a pH range of 7.2 to 7.4 when diluted to 1X, which aligns with physiological pH. This pH range is critical for biological reactions and cell culture applications, ensuring enzymes and cellular processes operate effectively. Regular pH checks during use can help ensure that your experiments yield reliable and reproducible results.

Can 10X PBS be used for rinsing cells in culture?

Absolutely, 10X PBS is commonly used to rinse cells in culture. Before trypsinization or during various assays, PBS effectively removes residual growth factors or serum without adversely affecting cell viability. Using PBS for rinsing cells helps maintain a stable environment, ensuring consistency in experimental conditions.

What makes Cepham Life Sciences' 10X PBS a good choice for labs?

Cepham Life Sciences' 10X PBS is crafted under stringent quality controls, ensuring a reliable product free from contaminants. This high standard of quality, combined with its convenient 500 mL packaging, makes it an excellent choice for researchers needing precise and effective reagents for their experiments. Researchers often prefer trusted brands to ensure their results are reproducible and consistent.

What is the best way to handle and dispense 10X PBS?

When handling and dispensing 10X PBS, it’s advisable to use sterile techniques to avoid contamination. Utilize pipettes or sterile syringes for accurate measurement, and dispense gradually to avoid splashes. This approach ensures that your workflow remains clean and your samples are protected, which is critical when performing sensitive biological experiments.

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PBS is a balanced, isotonic salt solution used for a variety of cell culture applications.

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Item #: 83742093

10X Phosphate Buffered Saline (PBS), 500 mL -from Cepham Life Sciences

Q: Where can I buy 10X Phosphate Buffered Saline (PBS), 500 mL from Cepham Life Sciences?

You can buy 10X Phosphate Buffered Saline (PBS), 500 mL from Cepham Life Sciences at Ubuy in South Africa. Ubuy offers a reliable platform to purchase this essential lab reagent, ensuring easy access to laboratory supplies for researchers and professionals seeking quality products for their experiments.

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PBS is a balanced, isotonic salt solution used for a variety of cell culture applications.

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What Stands Out

High Concentration

The 10X concentration of this phosphate buffered saline ensures maximum utility and convenience, allowing users to prepare desired dilutions with ease, saving time and enhancing efficiency in laboratory settings.

Large Volume

With a generous 500 mL volume, this PBS solution minimizes the frequency of purchases, catering to regular lab use while providing ample supply for multiple experiments and applications.

Superior Quality

Manufactured by Cepham Life Sciences, this PBS adheres to stringent quality standards, ensuring reliable results for varied biochemical and molecular biology applications, thus enhancing overall experimental accuracy and reproducibility.

Product Details

Container Type	Bottle
Item Form	Liquid
Volume	500 ml
Brand Name	Cepham Life Sciences
Manufacturer	Cepham Life Sciences
Brand	Cepham Life Sciences
Item Volume	500 ml
Package Information	Bottle
Item Weight	1.2 lbs (540 grams)

Who Should Buy?

Laboratory Researchers
Ideal for researchers conducting experiments requiring buffered saline solutions for cell culture or biochemical assays.
Biomedical Students
Useful for students in lab courses or projects involving cell biology, biochemistry, or immunology research.
Diagnostic Laboratories
Appropriate for clinical labs performing diagnostic tests where maintaining pH balance is crucial for accurate results.

Casual Users
Not suitable for general consumers or hobbyists without specialized knowledge of laboratory compounds and applications.

Product Description

10X Phosphate Buffered Saline (PBS), 500 mL -from Cepham Life Sciences

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Question: How to Shop 10X Phosphate Buffered Saline (PBS), 500 mL -from Online From Ubuy?

Answer: It’s easy to shop 10X Phosphate Buffered Saline (PBS), 500 mL -from online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.
Question: Is 10X Phosphate Buffered Saline (PBS), 500 mL -from Available to Shop Online in South Africa?

Answer: Yes, at Ubuy South Africa this product is available for you to shop at a reasonable price. The 10X Phosphate Buffered Saline (PBS), 500 mL -from is not available locally but you can trust us with our express shipping services.
Question: How Long Does It Take to Get Product After Placing the Order?

Answer: The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

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Features & Benefits

High-quality 10X Phosphate Buffered Saline from Cepham Life Sciences.
Sterilized with a 0.22 micron filter for optimal safety.
Ideal for biological research and cell culture applications.
Available in convenient sizes: 500 mL, 1L, and 1 Gal.
Perfect for washing, transporting, diluting, and preparing reagents.
pH of 7.4 ensures balanced conditions for your research.

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10X Phosphate Buffered Saline (PBS), 500 mL -from Cepham Life Sciences

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