What is RPMI 1640 and how is it used in cell culture?

RPMI 1640 is a widely used medium for the cultivation of various cell types, especially lymphoid cells. Its formulation provides essential nutrients, vitamins, and amino acids required for cell growth and maintenance. In laboratories, RPMI 1640 facilitates research in immunology and cancer studies, where a sterile and nutrient-rich environment is crucial for cell viability and proliferation.

What is the significance of L-glutamine in RPMI 1640?

L-glutamine is an amino acid critical for cell metabolism and protein synthesis. In RPMI 1640, it serves as a vital energy source, especially for rapidly dividing cells. Many cell lines, including lymphocytes, thrive in environments rich in L-glutamine, enabling researchers to obtain consistent and reproducible results in various experimental setups.

Why is sodium bicarbonate omitted in this version of RPMI 1640?

The omission of sodium bicarbonate in this formulation allows for more flexibility in pH adjustments. Without it, researchers can tailor the medium to specific experimental conditions or the requirements of certain cell lines. This versatility is particularly beneficial in environments where CO2 levels need to be controlled or when special buffering systems are employed.

How should RPMI 1640 with L-glutamine be stored?

RPMI 1640 media should be stored at 2-8°C and protected from light. Proper storage conditions help maintain the integrity of the components, ensuring their effectiveness during use. It's vital to check the medium for any signs of contamination or degradation before application, especially in sensitive experiments involving cell cultures.

What type of cells can be cultured using RPMI 1640?

RPMI 1640 is suitable for a variety of cell lines, particularly lymphocytes, hybridomas, and human leukemic cells. It supports the growth of both adherent and suspension cell types, making it a versatile choice in cell biology research. Researchers often use it in experiments related to immune responses, cancer, and drug testing.

Can RPMI 1640 with L-glutamine be used for primary cell cultures?

Yes, RPMI 1640 with L-glutamine can be effectively used for certain primary cell cultures, especially those derived from hematopoietic tissues. It provides the necessary nutrients for their growth. However, it's essential to optimize the culture conditions, including serum supplements, to support specific primary cell requirements.

Is RPMI 1640 with L-glutamine suitable for serum-free culture?

While RPMI 1640 with L-glutamine is commonly used with serum, it can be adapted for serum-free culture by adding appropriate supplements. Researchers often modify the formulation to create a serum-free environment that best supports their specific cell lines, enhancing reproducibility and control over experimental conditions.

What are the benefits of using pre-made RPMI 1640 in research?

Using pre-made RPMI 1640 simplifies the preparation process, reducing the risk of contamination and ensuring consistency across experiments. It saves valuable time for researchers, allowing them to focus on their core experiments rather than media preparation, thus enhancing overall laboratory efficiency.

How do I know if my RPMI 1640 is still usable?

To determine if RPMI 1640 is still usable, check the expiration date, observe for any discoloration or particulate matter, and perform a sterility test if possible. If the medium appears normal and is within its shelf life, it should be suitable for cell culture purposes. Always prioritize using fresh media to maintain optimal cell growth.

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Mediatech

RPMI 1640 is a modification of McCoy's 5A containing an increased level of inositol.

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Item #: 71679026

Mediatech 50-020-PB RPMI 1640, with L-glutamine, Without Sodium Bicarbonate

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You can purchase 50-020-PB RPMI 1640, with L-glutamine, Without Sodium Bicarbonate directly from Ubuy in South Africa. Ubuy offers a convenient platform to find this specific product along with various brands, ensuring you have access to high-quality laboratory media for your research needs.

Item #: 71679026

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RPMI 1640 is a modification of McCoy's 5A containing an increased level of inositol.

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What Stands Out

Cell Culture Support

RPMI 1640 promotes optimal cell growth, making it ideal for various cell lines, ensuring consistent and reliable experimental results in laboratories.

L-Glutamine Inclusion

The addition of L-glutamine provides essential nutrients, enhancing cell viability and proliferation, which is crucial for high-quality research outcomes.

Bicarbonate-Free Formula

Without sodium bicarbonate, this formulation eliminates pH variability, offering a stable environment for sensitive experiments and improving reproducibility across research studies.

Product Details

Mediatech 50-020-PB RPMI 1640 is a cell culture medium with lower documented Endotoxin content than other major suppliers.
Produced under the highest industry standards to ensure superior results.
Complete Certificate of Analysis available for each production lot, along with full formulation information.
Named after the site it was developed, the Roswell Park Memorial Institute.
Appropriate for cell types such as mammalian myeloma and hybridoma cells, human leukocytes, B and T lymphocytes, and human leukemic cells.
Ideal for suspension cultures or monolayer cultures.

Brand Name	Mediatech
Item Form	Powder
Age Range Description	Adult
Dosage Form	Capsule
Manufacturer	Mediatech, Inc., a Corning Subsidiary
Model Number	B003L95EA
Manufacturer Part Number	50-020-PB
Unit Count	1.0 Count
Number of Items	1
Brand	Mediatech
Age Range (Description)	Adult
Package Weight	20 Pound

Who Should Buy?

Cell Culture
Ideal for culturing a variety of mammalian cells in a controlled environment for research and experimentation.
Research Laboratories
Useful for laboratories focused on cell biology, immunology, and other life sciences requiring RPMI 1640 medium.
Experiments with L-glutamine
Beneficial for researchers needing L-glutamine in their media without sodium bicarbonate for specific cellular conditions.

Sodium Bicarbonate Based
Not suitable for cultures requiring sodium bicarbonate for CO2 buffering in a standard cell culture environment.

Product Description

About This Item

Introducing Mediatech 50-020-PB RPMI 1640, the ideal medium for your cellular research needs. Developed at the prestigious Roswell Park Memorial Institute, this modified version of McCoy's 5A offers a range of benefits for various cell types. Whether you're working with mammalian myeloma and hybridoma cells like mouse hybridomas, human leukocytes, or B and T lymphocytes, RPMI 1640 is the perfect fit. Its increased level of inositol ensures optimal cell growth and performance. Not only that, but RPMI 1640 is also highly suitable for suspension cultures or monolayer human leukemic cells.

You can expect consistent and reliable results every time, enabling you to advance your research with confidence. Mediatech's dedication to quality shines through in every bottle of the Mediatech 50-020-PB RPMI 1640. This carefully formulated medium is designed to provide optimal cell nutrition and support, making it the preferred choice of researchers worldwide. For convenience, this RPMI 1640 variant comes complete with L-glutamine but without sodium bicarbonate. This allows you to tailor the medium to your specific experimental requirements without compromising on quality. When it comes to your cellular research, trust Mediatech RPMI 1640 to deliver exceptional results.

Experience the difference today with the Mediatech 50-020-PB RPMI 1640 medium.

Dietary Supplement Disclaimer

Statements regarding dietary supplements have not been evaluated by the Food and Drug Administration and are not intended to diagnose, treat, cure, or prevent any disease or health condition.

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Question: How to Shop Mediatech 50-020-PB RPMI 1640, with L-glutamine, Online From Ubuy?

Answer: It’s easy to shop Mediatech 50-020-PB RPMI 1640, with L-glutamine, online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.
Question: Is Mediatech 50-020-PB RPMI 1640, with L-glutamine, Available to Shop Online in South Africa?

Answer: Yes, at Ubuy South Africa this product is available for you to shop at a reasonable price. The Mediatech 50-020-PB RPMI 1640, with L-glutamine, is not available locally but you can trust us with our express shipping services.
Question: How Long Does It Take to Get Product After Placing the Order?

Answer: The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

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Pros

High-quality formulation
Excellent cell growth support
Easy to use
Compatible with various applications
No sodium bicarbonate for flexibility

Cons

Might require additional buffering.

Product Price History

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Features & Benefits

Appropriate for various cell types including mammalian myeloma, hybridoma cells, human leukocytes, and B and T lymphocytes
Ideal for suspension cultures or monolayer human leukemic cells
Lower documented Endotoxin content than other major suppliers
Produced under high industry standards
Complete Certificate of Analysis available for each production lot with full formulation information

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Mediatech 50-020-PB RPMI 1640, with L-glutamine, Without Sodium Bicarbonate

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Product Description

About This Item

Dietary Supplement Disclaimer

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Mediatech Standards B003L95EA Editorial Review

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Product Price History

Important information

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