What is PBS [10X] Phosphate Buffered Saline used for?

PBS [10X] Phosphate Buffered Saline is commonly used in biological and biochemical laboratories. Its primary function is to maintain a stable pH and provide a buffer system for biological samples. This is crucial during enzyme reactions or cell culture processes. For example, when you're working with cells for experiments, using PBS helps preserve their viability and function. It’s also essential in washing cells or tissues before microscopy or analysis.

How do you dilute PBS [10X] for use?

To use PBS [10X], it needs to be diluted to a 1X working solution by mixing one part PBS [10X] with nine parts of distilled water. For instance, to create 100ml of 1X PBS, mix 10ml of PBS [10X] with 90ml of distilled water. This dilution is vital in ensuring the buffer maintains appropriate ionic concentration, making it effective for various laboratory applications, such as dilution of samples or providing a balanced environment for cells.

Is PBS [10X] suitable for cell culture?

Yes, PBS [10X] is suitable for cell culture applications. It is specifically designed to provide an isotonic environment that protects cells from osmotic shock. When working with cultured cells, PBS can be used for washing cells, removing serum, or suspending cells during experiments. This ensures that cells maintain their health and integrity during manipulations, facilitating better experimental outcomes.

Can PBS [10X] be used for molecular biology techniques?

Absolutely! PBS [10X] is widely employed in various molecular biology techniques, including PCR, Western blotting, and ELISA. It helps maintain the integrity of proteins and nucleic acids during experimental processes. For example, in Western blotting, it washes away unbound antibodies, ensuring specificity and enhancing results. Its buffering capacity ensures that reactions stay in optimal pH conditions.

What are the storage conditions for PBS [10X]?

PBS [10X] should be stored at room temperature or refrigerated, away from light. Proper storage ensures the stability of its components over time. It's best to keep it tightly sealed to prevent contamination. In laboratory scenarios, this helps avoid changes in composition that could affect experiments. Always check for any precipitate before use, as this could indicate degradation.

Is PBS [10X] sterile, and do I need to sterilize it before use?

PBS [10X] is typically supplied in sterile packaging, but it is crucial to check the label. If sterility is uncertain or if contamination is a concern, you can filter-sterilize it using a 0.22-micron filter. Sterilization is particularly important in sensitive applications like cell culture or when handling primary cells, ensuring that no unwanted microorganisms affect your results.

Are there any alternatives to PBS [10X]?

Yes, there are several alternatives to PBS [10X], such as TPS (Tris-Buffered Saline) or other proprietary buffers. However, the choice depends on the specific requirements of your experiment. PBS is preferred for its simplicity and compatibility with many protocols. In laboratory settings, if you need a buffer for specific applications like antibody dilutions, it’s crucial to choose one that maintains the desired ionic strength and pH.

What is the difference between PBS [1X] and PBS [10X]?

The main difference between PBS [1X] and PBS [10X] is their concentration. PBS [10X] is a concentrated solution that needs to be diluted before use, while PBS [1X] is ready to use. The 10X version contains higher concentrations of salts and buffers, making it more versatile for preparing larger volumes. In laboratory situations, researchers often keep PBS [10X] on hand for its convenience in creating custom dilutions.

Can I use PBS [10X] for rinsing laboratory instruments?

Yes, PBS [10X] can be used for rinsing laboratory instruments, particularly those used in biological applications. Its buffering capacity helps neutralize residual chemicals, ensuring a clean environment for experiments. For example, after using pipettes or culture plates, rinsing them with PBS can help avoid contamination from previous samples. It’s a common practice to ensure that instruments don’t interfere with subsequent experiments.

Where can I buy PBS [10X] Phosphate Buffered Saline, 500ml?

You can buy PBS [10X] Phosphate Buffered Saline, 500ml from Ubuy in South Africa. Ubuy offers a convenient shopping experience with a wide selection of laboratory supplies. You can easily find and order PBS online, ensuring quick access to this essential laboratory buffer for your research or educational needs.

How to Shop PBS [10X] (Phosphate Buffered Saline), 500ml Online From Ubuy?

It’s easy to shop PBS [10X] (Phosphate Buffered Saline), 500ml online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.

Is PBS [10X] (Phosphate Buffered Saline), 500ml Available to Shop Online in South Africa?

Yes, at Ubuy South Africa this product is available for you to shop at a reasonable price. The PBS [10X] (Phosphate Buffered Saline), 500ml is not available locally but you can trust us with our express shipping services.

How Long Does It Take to Get Product After Placing the Order?

The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

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PBS [10X] (Phosphate Buffered Saline), 500ml

10X PBS is a sterile, ready-to-use solution for all your experimental needs.

GBIOSCIENCES

Item #: 90237982

PBS [10X] (Phosphate Buffered Saline), 500ml

Item #: 90237982

ZAR 1805

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What Stands Out

High Concentration

The PBS [10X] solution provides a high concentration for efficient sample preparation, ideal for a variety of laboratory applications, ensuring precise results in experiments.

Ready to Use

Delivered in a convenient 500ml volume, this PBS solution is ready to use, saving researchers time and effort in their workflows, and facilitating smooth transitions between laboratory tasks.

Versatile Applications

Suitable for cell culture, molecular biology, and immunohistochemistry, this phosphate buffered saline addresses broad laboratory needs, making it an essential tool for scientists across multiple fields.

Product Details

Item Weight

1.1 lbs (500 grams)

Who Should Buy?

Laboratory Researchers
Ideal for researchers requiring a stable pH environment for biological experiments and cell culture applications.
Biotechnology Students
Students in biotechnology courses can utilize PBS for lab work, enhancing their understanding of buffer solutions.
Healthcare Professionals
Applicable for those in clinical settings needing a reliable buffer solution for sample preparations and diagnostics.

Home Use
Not suitable for household applications as PBS is specifically designed for laboratory and medical purposes.
Non-Scientific Users
Individuals without a scientific background may find it unnecessary and confusing for everyday tasks.
Environmental Use
Not appropriate for environmental applications or in natural habitats as it's intended for controlled laboratory settings.

Product Description

PBS [10X] (Phosphate Buffered Saline), 500ml

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Customer Questions & Answers

Question: How to Shop PBS [10X] (Phosphate Buffered Saline), 500ml Online From Ubuy?

Answer: It’s easy to shop PBS [10X] (Phosphate Buffered Saline), 500ml online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.
Question: Is PBS [10X] (Phosphate Buffered Saline), 500ml Available to Shop Online in South Africa?

Answer: Yes, at Ubuy South Africa this product is available for you to shop at a reasonable price. The PBS [10X] (Phosphate Buffered Saline), 500ml is not available locally but you can trust us with our express shipping services.
Question: How Long Does It Take to Get Product After Placing the Order?

Answer: The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

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Pros

Highly effective buffering capacity
Maintains pH stability
Great for biological applications
Pre-filtered for purity
Convenient 500ml size

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Limitations : For products shipped internationally, please note that any manufacturer warranty may not be valid; manufacturer service options may not be available; product manuals, instructions, and safety warnings may not be in destination country languages; the products (and accompanying materials) may not be designed in accordance with destination country standards, specifications, and labeling requirements; and the products may not conform to destination country voltage and other electrical standards (requiring use of an adapter or converter if appropriate). The recipient is responsible for assuring that the product can be lawfully imported to the destination country. When ordering from Ubuy or its affiliates, the recipient is the importer of record and must comply with all laws and regulations of the destination country.
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Features & Benefits

10-fold concentrated phosphate buffered saline.
Sterile filtered through a 0.2µm filter.
Dilute 1 to 10 in ultrapure water for use.
Produces a balanced solution with pH 7.4.
Ideal for various laboratory applications.
Convenient and easy to use.

PBS [10X] (Phosphate Buffered Saline), 500ml

ZAR 1805

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100% Original Products

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What Stands Out

Product Details

Who Should Buy?

Product Description

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GBIOSCIENCES Buffers Editorial Review

Customer Reviews & Ratings

Review this product

Pros

Product Price History

Important information

ZAR 1805

Features & Benefits

Fast
Shipping

Free
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