What is PBS [1X] (Phosphate Buffered Saline) used for?

PBS [1X] is a sterile solution that maintains a constant pH and osmotic balance for biological experiments. It is widely used in various applications, including cell culture, immunological assays, and molecular biology techniques. The buffered saline helps to stabilize cellular environments, making it crucial for processes like rinsing cells, diluting reagents, or preparing samples for analysis.

How is PBS [1X] prepared?

PBS [1X] can be prepared by diluting a concentrated form of phosphate buffer in distilled water. This involves mixing specific amounts of sodium chloride, potassium chloride, disodium hydrogen phosphate, and potassium dihydrogen phosphate. It’s important to adjust the pH to 7.4 for optimal performance in biological applications. Ready-to-use formulations are widely available for convenience.

Is PBS [1X] safe to use in laboratory settings?

Yes, PBS [1X] is considered safe for use in laboratory environments. It is non-toxic and compatible with a variety of biological systems. However, standard laboratory safety protocols should still be followed. Always handle chemical solutions with appropriate personal protective equipment and proper disposal methods for used materials to ensure a safe working environment.

Can PBS [1X] be stored, and if so, how?

PBS [1X] can be stored at room temperature or refrigerated to prevent microbial growth. It is advisable to keep it in a sterile container to ensure its integrity. If prepared in larger batches, consider aliquoting it into smaller portions for convenient use without compromising the entire solution. Once opened, usage within a reasonable timeframe is recommended to maintain its effectiveness.

What is the pH of PBS [1X]?

The pH of PBS [1X] is typically adjusted to around 7.4, which is optimal for most biological applications. This pH level closely mimics physiological conditions, making it an ideal choice for experiments involving cells or tissues. Maintaining the correct pH is crucial as it significantly affects enzyme activity and cellular processes.

Can PBS [1X] be used for washing cells?

Yes, PBS [1X] is frequently used to wash cells in various laboratory protocols. It helps to remove any residual media or serum components before further processing, such as staining or analysis. Rinsing with PBS ensures that experimental conditions are standardized and that results are reliable, particularly in assays where cell viability or functionality is being assessed.

Is PBS [1X] suitable for freezing cells?

PBS [1X] can be used as a diluent for cryoprotectants, but it is not suitable as a freezing medium by itself. When freezing cells, combine PBS with a cryoprotectant like dimethyl sulfoxide (DMSO) or glycerol that helps preserve cell viability during the freezing process. This combination minimizes ice crystal formation and enhances cell survival upon thawing.

What is the difference between PBS [1X] and PBS [10X]?

PBS [1X] is a diluted version of PBS [10X], which is a concentrated formulation. The main difference lies in their uses: PBS [10X] needs to be diluted to 1X before use in experiments. The concentration ensures you have a longer shelf life and can be prepared freshly as needed. Make sure to adjust the pH after dilution, if necessary.

Where can I buy PBS [1X] (Phosphate Buffered Saline), 1 Liter in South Africa?

You can purchase PBS [1X] (Phosphate Buffered Saline), 1 Liter from Ubuy, which offers a variety of laboratory supplies and reagents tailored for professionals. Ubuy often features numerous brands and formulations, providing you with options to meet your specific research needs. Check out their inventory to find the best fit for your laboratory.

How to Shop PBS [1X] (Phosphate Buffered Saline), 1 Liter Online From Ubuy?

It’s easy to shop PBS [1X] (Phosphate Buffered Saline), 1 Liter online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.

Is PBS [1X] (Phosphate Buffered Saline), 1 Liter Available to Shop Online in South Africa?

Yes, at Ubuy South Africa this product is available for you to shop at a reasonable price. The PBS [1X] (Phosphate Buffered Saline), 1 Liter is not available locally but you can trust us with our express shipping services.

How Long Does It Take to Get Product After Placing the Order?

The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

GBIOSCIENCES

PBS [1X] (Phosphate Buffered Saline), 1 Liter

1X PBS is a ready-to-use, sterile, pH adjusted solution perfect for your laboratory needs.

GBIOSCIENCES

Item #: 90790739

PBS [1X] (Phosphate Buffered Saline), 1 Liter

Item #: 90790739

ZAR 1529

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1X PBS is a ready-to-use, sterile, pH adjusted solution perfect for your laboratory needs.

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What Stands Out

Balanced Formula

PBS provides a stable pH environment, crucial for maintaining biological activity in cell cultures and biochemical assays, ensuring reliable experimental outcomes.

Versatile Application

Ideal for various laboratory processes including cell washing, diluting antibodies, and cell transport, making it a staple in research and clinical settings.

Bulk Convenience

Offered in a 1-liter volume, PBS reduces the need for frequent reordering, ensuring laboratories have ample supply for extended research projects.

Product Details

Item Weight

2.2 lbs (1 kg)

Who Should Buy?

Laboratory Researchers
Essential for cell culture, sample dilution, and various assays requiring a stable pH environment in biological studies.
Medical Professionals
Used in various diagnostic tests and medical procedures requiring a sterile and buffered saline solution.
Biotechnology Companies
Ideal for production processes involving proteins and antibodies that require consistent buffering in laboratory setups.

Home Use
Not suitable for household applications due to its specific usage in controlled laboratory environments.
Animal Consumption
Not intended for ingestion or use in food products; can be harmful if consumed by animals or humans.
Non-Biological Applications
Lacks efficacy in applications outside of biological sciences like agriculture or industrial use scenarios.

Product Description

PBS [1X] (Phosphate Buffered Saline), 1 Liter

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Customer Questions & Answers

Question: How to Shop PBS [1X] (Phosphate Buffered Saline), 1 Liter Online From Ubuy?

Answer: It’s easy to shop PBS [1X] (Phosphate Buffered Saline), 1 Liter online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.
Question: Is PBS [1X] (Phosphate Buffered Saline), 1 Liter Available to Shop Online in South Africa?

Answer: Yes, at Ubuy South Africa this product is available for you to shop at a reasonable price. The PBS [1X] (Phosphate Buffered Saline), 1 Liter is not available locally but you can trust us with our express shipping services.
Question: How Long Does It Take to Get Product After Placing the Order?

Answer: The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

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Pros

Great for cell culture
Stable pH levels
Easy to use
Compatible with various applications
High-quality ingredients

Cons

Bottle design could be improved.

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Limitations : For products shipped internationally, please note that any manufacturer warranty may not be valid; manufacturer service options may not be available; product manuals, instructions, and safety warnings may not be in destination country languages; the products (and accompanying materials) may not be designed in accordance with destination country standards, specifications, and labeling requirements; and the products may not conform to destination country voltage and other electrical standards (requiring use of an adapter or converter if appropriate). The recipient is responsible for assuring that the product can be lawfully imported to the destination country. When ordering from Ubuy or its affiliates, the recipient is the importer of record and must comply with all laws and regulations of the destination country.
Not all the products listed on Ubuy are for sale, as Ubuy is a global search engine. Products are subject to export/trade regulations.

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Features & Benefits

Ready-to-use sterile solution.
pH adjusted to 7.4 for optimal performance.
Contains essential components: 8mM NaH2PO4, 150mM NaCl, 3mM KCl, 2mM KH2PO4.
Sterile filtered through 0.2µm filter.
Ideal for various laboratory applications.
Convenient formulation saves time and increases productivity.

PBS [1X] (Phosphate Buffered Saline), 1 Liter

ZAR 1529

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Important information

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