What is PBS (Phosphate Buffered Saline) and its uses?

PBS is a buffer solution commonly used in biological research to maintain pH and osmotic balance. It helps dilute cells or tissues without disrupting their function, making it ideal for applications like cell culture and immunoassays. Its stability at pH 7.4 is crucial, especially in experiments involving enzymes or proteins that require a neutral environment.

Is 1X PBS suitable for cell culture?

Yes, 1X PBS is widely used in cell culture as it mimics the physiological conditions of cells, providing an environment that maintains cell viability. It is particularly useful for rinsing cells, diluting reagents, or as a buffer during enzyme assays, ensuring that cellular functions remain intact during experimental procedures.

What does RNase and DNase free mean in PBS?

Being RNase and DNase free indicates that the PBS solution is free from enzymes that can degrade RNA and DNA, making it essential for molecular biology applications. This quality is important when working with nucleic acids, as it ensures the integrity of your samples and prevents contamination, which can compromise your research results.

Why is sterile filtered PBS important?

Sterile filtered PBS is crucial for preventing microbial contamination in experiments, especially those involving cell cultures or delicate biological assays. The filtration removes pathogens, ensuring that the samples or cultures you are exposing to the PBS remain uncontaminated, ultimately preserving experimental integrity and reliability.

Can PBS be used for preparing samples for Western blotting?

Certainly! PBS is often used for diluting primary and secondary antibodies in Western blotting procedures. Its buffering capacity helps maintain the appropriate pH during the assay, which is essential for optimal antibody binding and accurate detection of proteins.

What are the storage recommendations for 1X PBS?

1X PBS should be stored at 2-8°C to maintain its effectiveness and prevent microbial growth. While it is stable for several months, it is advisable to check for any signs of contamination or cloudiness before use, especially if opened, to ensure the reliability of your experiments.

How can PBS be used in immunohistochemistry?

In immunohistochemistry, PBS serves as a diluent for antibodies and solutions used during tissue staining. It maintains a constant pH, ensuring that antigen-antibody interactions occur under optimal conditions, thus enhancing the clarity and accuracy of staining results in tissue sections.

Can 1X PBS be used in conjunction with other buffers?

Yes, 1X PBS can be mixed with other buffers depending on the specific requirements of the experiment. For instance, it can be combined with Triton X-100 for cell permeabilization in immunofluorescence or other reagents to adjust ionic strength or pH for specialized applications in molecular biology.

What volume is available for PBS solutions and how does it affect usage?

PBS solutions are commonly available in different volumes, such as 500 mL, which is ideal for routine laboratory applications. The 500 mL volume provides ample reagent for multiple experiments while ensuring you have enough buffer to dilute reagents or cells without running out too quickly, thus enhancing experimental efficiency.

Where can I buy 1X PBS Phosphate Buffered Saline in South Africa?

You can purchase 1X PBS Phosphate Buffered Saline, pH 7.4 - Molecular Biology Grade - RNaseDNase Free, Sterile Filtered - 500 mL conveniently from Ubuy. They offer a reliable platform for sourcing high-quality lab materials, ensuring you receive the products needed for your research and experiments efficiently.

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Is 1X PBS (Phosphate Buffered Saline), pH 7.4 - Available to Shop Online in South Africa?

Yes, at Ubuy South Africa this product is available for you to shop at a reasonable price. The 1X PBS (Phosphate Buffered Saline), pH 7.4 - is not available locally but you can trust us with our express shipping services.

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The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

UFC Bio

1X PBS (Phosphate Buffered Saline), pH 7.4 - Molecular Biology Grade - RNase/DNase Free, Sterile Filtered - 500 mL

Research-grade solution for various research purposes.

UFC Bio

Item #: 63455949

1X PBS (Phosphate Buffered Saline), pH 7.4 - Molecular Biology Grade - RNase/DNase Free, Sterile Filtered - 500 mL

Item #: 63455949

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What Stands Out

Molecular Biology Grade

Specifically formulated for molecular biology applications, ensuring optimal performance and reliability in experiments involving nucleic acids and proteins.

Sterile and Safe

Sterile filtered and RNase/DNase free, this solution minimizes contamination risk, safeguarding your samples for accurate and precise results.

Convenient Size

The 500 mL volume provides ample supply for various laboratory tasks, reducing the need for frequent replenishment and enhancing workflow efficiency.

Product Details

1X PBS Buffer
Concentration: 137mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4, pH 7.4 @ 24°C
Molecular Biology Grade, RNase/DNase Free, Sterile Filtered
Size: 500mL
Expiration Date: 2 years from manufacturing date
Made in USA

Package Weight

1.5 Pound

Who Should Buy?

Molecular Biologists
Ideal for applications requiring a stable pH and sterile conditions in biological experiments or sample preparation.
Cell Culture
Perfect for maintaining osmotic balance and providing essential ions for cultured cells in laboratory settings.
Immunology Studies
Useful in immunological assays as a diluent or wash buffer, minimizing background and preserving sample integrity.

Field Research
Not suitable for outdoor use where refrigeration or controlled conditions cannot be ensured during transport.

Product Description

1X PBS (Phosphate Buffered Saline), pH 7.4 - Molecular Biology Grade - RNase/DNase Free, Sterile Filtered - 500 mL

About This Item

Introducing our high-quality 1X PBS Phosphate Buffered Saline, the ideal solution for all your molecular biology needs. With a pH of 7.4, this Molecular Biology Grade formula ensures optimal performance and accurate results in your research. Designed specifically for scientific research purposes, Ubuy's 1X PBS Buffer is carefully manufactured to be free from contaminants such as RNase and DNase. This guarantees the integrity of your samples, making it perfect for sensitive applications. Our sterile filtration process ensures that each 500 mL bottle of 1X PBS Buffer is free from unwanted particles and microorganisms, providing you with a reliable and safe solution every time you use it.

Use it with confidence in your laboratory, knowing that it has undergone stringent quality control measures. Whether you're working with proteins, DNA, or RNA, Ubuy's Molecular Biology Grade 1X PBS Buffer is an essential tool in your laboratory. Its precise pH balance allows for optimal interaction between molecules, facilitating accurate experimental results. With Ubuy, you can trust that our Molecular Biology Grade products are produced to the highest standards of quality and purity. Our dedication to delivering reliable and consistent solutions has made us a trusted choice among scientists and researchers. Don't compromise on the integrity of your research.

Choose Ubuy's 1X PBS Phosphate Buffered Saline for all your molecular biology needs. Experience the difference in quality and performance that our products offer. Shop now and take your research to new heights!.

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Question: How to Shop 1X PBS (Phosphate Buffered Saline), pH 7.4 - Online From Ubuy?

Answer: It’s easy to shop 1X PBS (Phosphate Buffered Saline), pH 7.4 - online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.
Question: Is 1X PBS (Phosphate Buffered Saline), pH 7.4 - Available to Shop Online in South Africa?

Answer: Yes, at Ubuy South Africa this product is available for you to shop at a reasonable price. The 1X PBS (Phosphate Buffered Saline), pH 7.4 - is not available locally but you can trust us with our express shipping services.
Question: How Long Does It Take to Get Product After Placing the Order?

Answer: The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

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Pros

RNase/DNase free for purity
Stable pH for experiments
Sterile filtered for safety
Widely used in labs
Convenient 500 mL size

Cons

Some users report inconsistency in formulation.

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Last Updated on August 6, 2026

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1X PBS (Phosphate Buffered Saline), pH 7.4 - Molecular Biology Grade - RNase/DNase Free, Sterile Filtered - 500 mL

ZAR 704

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