What is TRIS Hydrochloride used for?

TRIS Hydrochloride, also known as Trishydroxymethyl aminomethane HCl, is primarily used as a buffering agent in biochemical and molecular biology applications. It helps maintain a stable pH in various laboratory settings, which is crucial for effective enzyme reactions, protein stability, and overall experiment reliability. For instance, in a typical DNA electrophoresis experiment, using TRIS as a buffer can significantly improve the clarity and sharpness of the separated bands.

How should I store TRIS Hydrochloride?

TRIS Hydrochloride should be stored in a cool, dry place, away from direct sunlight and moisture. Proper storage helps preserve its chemical stability and efficacy. It's advisable to keep it in an airtight container to prevent exposure to humidity which may cause degradation. Laboratories using TRIS often store it in standardized glass or plastic containers to maintain its integrity over time, ensuring consistent results in experiments.

Is TRIS Hydrochloride harmful if ingested?

While TRIS Hydrochloride is generally regarded as safe when handled properly in a laboratory environment, it is not meant for ingestion and can be harmful if swallowed. Exposure can lead to irritation of the respiratory tract or skin; therefore, protective gear such as gloves and goggles should be used during handling. Understanding its safety data sheet (SDS) is vital for users handling TRIS in lab settings to ensure all safety precautions are followed.

What is the recommended concentration of TRIS Hydrochloride for buffer solutions?

The recommended concentration of TRIS Hydrochloride for buffer solutions typically ranges from 0.1 M to 1 M, depending on the application. Buffer solutions created with TRIS at a concentration of 0.1 M have been widely used for DNA and protein analysis. Adjusting the pH of TRIS solutions is also done to suit specific processes, allowing for flexibility in experimental design, as it can adequately stabilize pH levels between 7.0 to 9.0.

Can I use TRIS Hydrochloride in cell culture?

Yes, TRIS Hydrochloride can be used in cell culture applications; however, it’s essential to ensure compatibility with the specific cell line being cultivated. It can function as a buffering agent in culture media, helping to maintain an optimal pH level, which is crucial for cell growth and viability. Researchers should consider the specific requirements of their cell culture systems when incorporating TRIS to optimize growth conditions effectively.

What is the molecular weight of TRIS Hydrochloride?

The molecular weight of TRIS Hydrochloride is approximately 157.6 g/mol. This information is particularly significant for researchers and chemists when calculating molar concentrations and preparing solutions for various experimental protocols. A clear understanding of the molecular weight allows for precise measurements when preparing buffered solutions, ensuring reproducibility in scientific research.

What measures should I take if I spill TRIS Hydrochloride?

In the event of a spill, it's crucial to act promptly to minimize hazards. First, evacuate the area and ensure adequate ventilation. Use appropriate personal protective equipment (PPE), such as gloves and a mask, and use absorbent materials to clean up the spill. Dispose of waste materials according to local regulations. Following spill protocols ensures a safe laboratory environment and reduces risks associated with handling chemicals like TRIS Hydrochloride.

Is TRIS Hydrochloride soluble in water?

Yes, TRIS Hydrochloride is highly soluble in water. It typically dissolves easily to form a clear solution, making it convenient for laboratory use. Its solubility makes it an excellent choice for preparing buffer solutions, enabling researchers to create consistent and effective environments for biochemical reactions. For optimal results, TRIS solutions should be prepared with high-purity water to avoid contamination during experiments.

What alternative buffers can I use instead of TRIS Hydrochloride?

Alternatives to TRIS Hydrochloride include phosphate-buffered saline (PBS) and citrate buffers. Each alternative offers unique properties that might be more suitable depending on the specific biological or chemical processes. For instance, PBS is commonly used in cell culture applications due to its physiological pH and ionic strength, while citrate buffers may be preferable for specific enzymatic reactions. Choosing the right buffer helps optimize experimental conditions and outcomes.

Is TRIS Hydrochloride [Tris(hydroxymethyl) aminomethane Available to Shop Online in South Africa?

Yes, at Ubuy South Africa this product is available for you to shop at a reasonable price. The TRIS Hydrochloride [Tris(hydroxymethyl) aminomethane is not available locally but you can trust us with our express shipping services.

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The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

RPI

TRIS Hydrochloride [Tris(hydroxymethyl) aminomethane HCl], 500 Grams

Highly purified Tris(hydroxymethyl) aminomethane hydrochloride for molecular biology applications.

RPI

Item #: 28402580

TRIS Hydrochloride [Tris(hydroxymethyl) aminomethane HCl], 500 Grams

Q: Where can I buy TRIS Hydrochloride [Trishydroxymethyl aminomethane HCl], 500 Grams?

You can buy TRIS Hydrochloride [Trishydroxymethyl aminomethane HCl], 500 Grams from Ubuy, which operates in South Africa. Ubuy offers a reliable platform for chemical supplies and laboratory products, ensuring quality and competitive options for researchers and professionals alike. You can easily explore their inventory and place your order online to meet your laboratory needs.

Item #: 28402580

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What Stands Out

High Purity

TRIS Hydrochloride offers premium quality with high purity ensuring reliable performance in laboratory applications, making it ideal for biochemical research and molecular biology.

Optimal Buffering

Designed for effective pH stabilization, TRIS Hydrochloride maintains consistent pH levels in laboratory experiments, significantly improving the reliability of results in various protocols.

Versatile Applications

This product is suitable for a range of scientific applications, including electrophoresis and protein studies, providing researchers with a dependable tool for diverse experimental setups.

Product Details

Product is sold for research or further manufacturing use only, not for food or drug use.
Chemical Formula: C4H11NO3-HCl
CAS Number: 1185-53-1
Molecular Weight: 157.6
Common Name: Tris(hydroxymethyl) aminomethane hydrochloride

Container Type	Bottle
Item Weight	8.96 ounces (254.02 grams)
Brand Name	RPI
Manufacturer	RPI, Corp.
Part Number	T60050-500.0
Brand	RPI
Package Information	Bottle

Who Should Buy?

Biochemical Researchers
Ideal for researchers conducting experiments involving buffer solutions and enzyme activity in biological studies.
Laboratory Technicians
Essential for technicians preparing electrophoresis gels and running various biochemical assays in laboratories.
Educational Institutions
Useful for schools and universities offering courses in biochemistry and molecular biology, enhancing student learning.

General Consumers
Not suitable for casual consumers without laboratory needs or knowledge of biochemical applications.
Home Use
Not advisable for home settings due to potential safety risks and lack of appropriate application.
Non-Scientific Fields
Individuals in unrelated fields may find no practical use for this specialized chemical reagent.

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Question: How to Shop TRIS Hydrochloride [Tris(hydroxymethyl) aminomethane Online From Ubuy?

Answer: It’s easy to shop TRIS Hydrochloride [Tris(hydroxymethyl) aminomethane online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.
Question: Is TRIS Hydrochloride [Tris(hydroxymethyl) aminomethane Available to Shop Online in South Africa?

Answer: Yes, at Ubuy South Africa this product is available for you to shop at a reasonable price. The TRIS Hydrochloride [Tris(hydroxymethyl) aminomethane is not available locally but you can trust us with our express shipping services.
Question: How Long Does It Take to Get Product After Placing the Order?

Answer: The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

RPI Buffers Editorial Review

TRIS Hydrochloride is a high-quality product that is well-regarded by customers. It is commonly used in molecular biology research for buffer preparation, protein purification, and DNA extraction. The product has received positive feedback for its purity and effectiveness. The easy-to-use packaging and clear instructions make it convenient for laboratory use. Overall, TRIS Hydrochloride is a reliable choice for researchers in the field.

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High-quality and reliable product
Used for various molecular biology applications
Received positive feedback for purity and effectiveness
Convenient packaging and clear instructions

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TRIS Hydrochloride [Tris(hydroxymethyl) aminomethane HCl], 500 Grams

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