What is 5M Ammonium Acetate Buffer Solution used for?

5M Ammonium Acetate Buffer Solution is primarily utilized in molecular biology, biochemistry, and analytical chemistry as a buffering agent. It helps maintain a stable pH during various experiments, particularly in nucleic acid isolation and protein purification. This stability is crucial for minimizing enzymatic degradation, ensuring authenticity in results. For instance, if you are conducting gel electrophoresis, using this buffer can help in maintaining optimal pH levels, thus enhancing the resolution of your sample separates.

How should I store 5M Ammonium Acetate Buffer Solution?

5M Ammonium Acetate Buffer Solution should be stored in a cool, dark place, ideally at temperatures between 2-8°C. It’s important to keep it tightly sealed to prevent contamination and evaporation of the buffer components. If stored correctly, the solution retains its effectiveness for a long time. Scientists often keep it in lab fridges alongside other reagents to ensure they have quick access when conducting experiments such as PCR or gel electrophoresis.

Is the 5M Ammonium Acetate Buffer Solution suitable for all types of experiments?

While versatile, 5M Ammonium Acetate Buffer Solution may not be suitable for every type of experiment. Its specific pH range and ionic strength make it ideal for certain DNA and RNA applications, but not for assays sensitive to specific buffer ions. Therefore, it’s essential to consider the requirements of your particular protocol. For instance, it’s commonly used in DNA extraction but might not be recommended for enzyme reactions that require pure water conditions.

What safety precautions should I take when using 5M Ammonium Acetate Buffer Solution?

When using 5M Ammonium Acetate Buffer Solution, always wear appropriate personal protective equipment such as gloves, goggles, and lab coats. Although it is generally considered safe, it can be irritating to skin and eyes. It's crucial to work in a well-ventilated area and to understand the Safety Data Sheet (SDS) for handling and disposal. For example, if spills occur, protocols for neutralizing and cleaning should be in place to minimize exposure and environmental impact.

Can I dilute 5M Ammonium Acetate Buffer Solution for other applications?

Yes, 5M Ammonium Acetate Buffer Solution can be diluted to suit various applications. Depending on your experimental needs, you can prepare lower concentrations, such as 1M or 0.5M, by mixing with deionized water. This flexibility allows researchers to tailor conditions based on specific protocols, such as preparing reaction buffers or sample diluents. Always ensure that you calculate dilution ratios accurately to maintain effective concentrations.

What are the common applications of Ammonium Acetate Buffer Solution?

Common applications of 5M Ammonium Acetate Buffer Solution include DNA and RNA extraction, protein purification, and chromatography. Its buffering capacity is particularly useful in maintaining pH stability during these processes. For example, in RNA isolation, using the correct buffer ensures that the nucleic acids remain intact and are not degraded. Similarly, in protein studies, this solution helps maintain functional and structural integrity during analyses.

Is 5M Ammonium Acetate Buffer Solution compatible with other reagents?

5M Ammonium Acetate Buffer Solution is compatible with many biological reagents, making it a versatile component in many procedures. However, it’s important to check compatibility with specific enzymes or substrates, as some may be sensitive to ammonium ions. For example, it works well with DNases and RNases, enhancing their activity in molecular biology applications. However, always perform preliminary tests when using it alongside new reagents.

Can 5M Ammonium Acetate Buffer Solution be used in protein crystallization?

Yes, 5M Ammonium Acetate Buffer Solution is often used in protein crystallization experiments. Its buffering properties help maintain a stable pH which is critical during crystallization trials. This stability aids in achieving higher-quality crystal formations. Researchers often assess various buffer conditions, including ammonium acetate, to optimize crystallization setups that can lead to better structural insights of proteins.

How does 5M Ammonium Acetate Buffer Solution compare to other buffer systems?

5M Ammonium Acetate Buffer Solution offers distinct advantages over other buffer systems, particularly in its simplicity and effectiveness at maintaining pH. Unlike phosphate buffers, it minimizes precipitation issues in ionic interactions. This makes it ideal for DNA and RNA manipulation. Researchers may choose ammonium acetate buffers over others in applications like gel electrophoresis for sharper band resolution and better sample integrity.

Where can I buy 5M Ammonium Acetate Buffer Solution, 250 mL in South Africa?

You can buy the 5M Ammonium Acetate Buffer Solution, 250 mL from Ubuy in South Africa. Ubuy provides a convenient online shopping experience, allowing you to browse a selection of laboratory chemicals and reagents to meet your research needs. You will find detailed product information and the option to procure the buffer solution with assurance of quality.

How to Shop 5M Ammonium Acetate Buffer Solution, 250 mL Online From Ubuy?

It’s easy to shop 5M Ammonium Acetate Buffer Solution, 250 mL online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.

Is 5M Ammonium Acetate Buffer Solution, 250 mL Available to Shop Online in South Africa?

Yes, at Ubuy South Africa this product is available for you to shop at a reasonable price. The 5M Ammonium Acetate Buffer Solution, 250 mL is not available locally but you can trust us with our express shipping services.

How Long Does It Take to Get Product After Placing the Order?

The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

eCon Lab Supply

5M Ammonium Acetate Buffer Solution, 250 mL

Our 5M Ammonium acetate solution is prepared in molecular biology grade/ultrapure water, filter sterilized with 0.22 µm filter and DNase/RNase/Protease Free.

eCon Lab Supply

Item #: 86739675

5M Ammonium Acetate Buffer Solution, 250 mL

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Item #: 86739675

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What Stands Out

High Concentration

This 5M Ammonium Acetate solution ensures a powerful buffering capacity, ideal for sensitive applications in biochemistry and molecular biology.

Large Volume

With 250 mL, it provides ample quantity for extensive laboratory use, minimizing the need for frequent repurchases and ensuring consistency in experiments.

Versatile Use

Suitable for a variety of laboratory applications, including nucleic acid precipitation and protein purification, making it a valuable addition to your lab supplies.

Product Details

Container Type	Bottle
Item Form	Liquid
Item Weight	8 ounces (226.8 grams)
Volume	250 ml
Brand Name	eCon Lab Supply
Manufacturer	Ciro Mfg
Included Components	Bottle
Part Number	23.105.92
Brand	eCon Lab Supply
Item Volume	250 ml
Package Information	Bottle

Who Should Buy?

Biochemical Research
Ideal for researchers conducting experiments in biochemistry requiring precise pH control and stable buffering capacity.
Molecular Biology
Useful for molecular biologists performing nucleic acid extraction and purification processes, enhancing yield and purity.
Analytical Chemistry
Beneficial for analytical chemists who need a reliable buffer for titrations and chromatography applications.

General Household Use
Not suitable for general household purposes or applications outside scientific research and laboratory environments.

Product Description

5M Ammonium Acetate Buffer Solution, 250 mL

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Question: How to Shop 5M Ammonium Acetate Buffer Solution, 250 mL Online From Ubuy?

Answer: It’s easy to shop 5M Ammonium Acetate Buffer Solution, 250 mL online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.
Question: Is 5M Ammonium Acetate Buffer Solution, 250 mL Available to Shop Online in South Africa?

Answer: Yes, at Ubuy South Africa this product is available for you to shop at a reasonable price. The 5M Ammonium Acetate Buffer Solution, 250 mL is not available locally but you can trust us with our express shipping services.
Question: How Long Does It Take to Get Product After Placing the Order?

Answer: The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

eCon Lab Supply Buffers Editorial Review

The 5M Ammonium Acetate Buffer Solution, packaged in a convenient 250 mL bottle, is essential for various laboratory applications requiring precise buffering capacity. This liquid solution, manufactured by Ciro Mfg under the reputable eCon Lab Supply brand, weighs 8 ounces and is designed for easy handling and storage. It provides a reliable option for maintaining pH stability in experiments. Users appreciate its good quality for various applications, making it a practical choice for laboratories needing effective buffering solutions. The container design facilitates easy pouring, ensuring no spillage during usage.

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Pros

Easy-to-use liquid form
Comes in a convenient bottle
Reliable buffering capacity
Good for various lab applications
Lightweight and portable

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Limited volume in the bottle

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5M Ammonium acetate solution
Prepared in molecular biology grade/ultrapure water
Filter sterilized with 0.22 µm filter
DNase/RNase/Protease Free
Used in DNA and RNA purification, biological buffers, chemical analysis, in pharmaceuticals, and in preserving foods

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5M Ammonium Acetate Buffer Solution, 250 mL

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eCon Lab Supply Buffers Editorial Review

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