What is 50X TAE Buffer Tris-Acetate-EDTA used for?

50X TAE Buffer Tris-Acetate-EDTA is commonly used in molecular biology applications, particularly for DNA and RNA gel electrophoresis. This buffer maintains a stable pH and optimal ionic strength, facilitating efficient separation and visualization of nucleic acids during electrophoresis. Researchers utilize this buffer for preparing agarose gels and running electrophoresis to analyze DNA fragments, test RNA quality, or conduct DNA sequencing.

How do I prepare working solutions from 50X TAE Buffer?

To prepare a working solution from 50X TAE Buffer, dilute it with distilled water. For a standard 1X working solution, combine 1 part of 50X TAE Buffer with 49 parts of distilled water, resulting in a total of 50 mL. This dilution is crucial for achieving the proper ionic concentration for successful electrophoresis. This process is applicable for various laboratory setups where precise measurements of nucleic acids are needed.

Can I store 50X TAE Buffer long-term?

Yes, 50X TAE Buffer can be stored long-term if kept at appropriate conditions. For optimal stability, store it in a cool, dark place, preferably at room temperature, or refrigerate it. However, avoid freezing the buffer as it can affect the buffer's performance. Proper storage ensures its effectiveness in various applications, such as DNA extraction and analysis, allowing researchers to maintain a consistent workflow over time.

Is 50X TAE Buffer suitable for RNA electrophoresis?

Yes, 50X TAE Buffer is suitable for RNA electrophoresis as it provides an effective medium for separation. The buffer’s ion composition aids in the resolution of RNA molecules, allowing for clearer results during gel electrophoresis. Additionally, it ensures that RNA maintains its structural integrity, making it ideal for applications in gene expression studies and other molecular biology experiments involving RNA.

What volume of 50X TAE Buffer do I need for a standard gel?

For a standard gel, the volume of 50X TAE Buffer needed will depend on the total volume of gel you wish to prepare. Typically, for a 100 mL agarose gel, you would use 2 mL of 50X TAE Buffer and add 98 mL of distilled water. This ensures that the final buffer concentration in the gel is at 1X, allowing for effective nucleic acid separation during electrophoresis.

Can 50X TAE Buffer be reused after gel electrophoresis?

While technically possible, reusing 50X TAE Buffer after gel electrophoresis is not recommended due to potential contamination and changes in ionic concentration. Each use can introduce impurities that may skew results in subsequent experiments. Fresh buffer ensures consistent performance, especially for critical applications like cloning and sequencing where precision is essential.

Does 50X TAE Buffer require pH adjustment?

50X TAE Buffer is generally prepared at a neutral pH of approximately 8.0 and does not require further adjustment when used directly from the bottle. If diluting for specific applications, it’s advisable to verify the pH, especially in sensitive experiments where pH can impact nucleic acid stability. Maintaining the correct pH is essential for optimal results in electrophoresis.

What are the alternatives to 50X TAE Buffer?

Alternatives to 50X TAE Buffer include TBE (Tris-Borate-EDTA) Buffer and others like SSC (Saline-Sodium Citrate) Buffer. Each buffer type has its unique advantages; for instance, TBE is often used for high-resolution separation of DNA fragments. Researchers may choose based on factors such as the type of nucleic acids being analyzed or the specific requirements of their experiment.

How does 50X TAE Buffer compare to 1X TAE Buffer?

50X TAE Buffer is a concentrated form, while 1X TAE Buffer is the diluted, working solution used for electrophoresis. The 50X concentration allows for greater convenience, as only a small amount is required to prepare a larger volume of 1X buffer. This concentration flexibility is beneficial for laboratories needing to conserve space or resources while preparing buffer solutions for multiple experiments.

Where can I buy 50X TAE Buffer Tris-Acetate-EDTA, 1L in South Africa?

You can purchase 50X TAE Buffer Tris-Acetate-EDTA, 1L at Ubuy. Ubuy offers a comprehensive selection of laboratory reagents and supplies, making it a convenient choice for researchers looking for quality products. Their user-friendly interface enables easy finding and ordering of essential lab materials, ensuring you have what you need for your experiments.

How to Shop 50X TAE Buffer (Tris-Acetate-EDTA), 1L Online From Ubuy?

It’s easy to shop 50X TAE Buffer (Tris-Acetate-EDTA), 1L online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.

Is 50X TAE Buffer (Tris-Acetate-EDTA), 1L Available to Shop Online in South Africa?

Yes, at Ubuy South Africa this product is available for you to shop at a reasonable price. The 50X TAE Buffer (Tris-Acetate-EDTA), 1L is not available locally but you can trust us with our express shipping services.

How Long Does It Take to Get Product After Placing the Order?

The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

eConLabSupply

50X TAE Buffer: Provides electrical conductivity, inhibits nucleases, and protects DNA during electrophoresis.

eConLabSupply

Item #: 66013072

50X TAE Buffer (Tris-Acetate-EDTA), 1L

Item #: 66013072

ZAR 1913

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What Stands Out

High-Quality Buffer

This 50X TAE buffer ensures reliable and reproducible performance in nucleic acid experiments, providing an optimal environment for DNA and RNA stabilization during gel electrophoresis.

Concentrated Formula

The 50X concentration allows for easy dilution to meet various experimental needs, minimizing storage space while maximizing efficiency and cost-effectiveness for high-throughput labs.

Versatile Applications

Ideal for diverse applications including agarose gel electrophoresis and molecular biology research, this buffer caters to a broad range of laboratory needs, enhancing its value for various scientific studies.

Product Details

Manufactured in an ISO 9001 and 13485 certified facility in the USA
For research use only. Not for human or animal clinical/therapeutic use.
Manufactured under rigorous quality control tests in a certified clean room environment
Used for running electrophoresis of nucleic acids in gels
EDTA protects DNA from nucleases during electrophoresis
Lower buffering capacity compared to TBE buffer

Item Weight

2.2 lbs (1 kg)

Who Should Buy?

Molecular Biologists
Ideal for molecular biologists conducting gel electrophoresis on DNA, providing optimal conditions for nucleic acid separation.
Academic Research
Perfect for academic labs performing DNA analysis and cloning, ensuring high integrity of samples during experimentation.
Educational Institutions
Useful for teaching laboratories in schools and universities, facilitating hands-on learning about genetic engineering and biochemistry.

Non-Biological Labs
Not suitable for labs focused on non-biological fields as its applications are specifically for molecular biology and genetics.
Small Volume Users
May not be ideal for users needing smaller quantities, as it comes in a large 1L format, potentially wasteful.
Casual Users
Not recommended for casual hobbyists due to the complexity of use and the need for proper handling and knowledge.

Product Description

50X TAE Buffer (Tris-Acetate-EDTA), 1L

About This Item

Introducing our 50X TAE Buffer Tris-Acetate-EDTA, a must-have product for all your nucleic acid electrophoresis needs. Our 50X TAE Buffer is specifically designed to provide optimum electrical conductivity and pH maintenance during gel electrophoresis. It contains a high concentration of Tris, Acetic acid, and EDTA to ensure accurate and efficient separation of nucleic acids. Why is this buffer essential? Firstly, the EDTA present in the buffer acts as a powerful inhibitor of metal-dependent nucleases by chelating divalent cations such as Ca2+ and Mg2+.

This protects the integrity of your DNA during the electrophoresis process, giving you reliable and accurate results every time. Not only suitable for genomic and large supercoiled DNA, but our 50X TAE Buffer can also be used as a gel preparation buffer. Its lower buffering capacity, compared to TBE buffer, makes it ideal for applications where faster dsDNA runs are desired. To use our 50X TAE Buffer, simply dilute it with molecular biology grade water to a 1X concentration before running your electrophoresis.

This ensures that the buffer is at the optimal concentration for your experiments. For optimal performance, we recommend periodic replacement of the buffer during prolonged electrophoresis sessions. This helps maintain the buffer's effectiveness and ensures consistent results. So, whether you're a research scientist, a laboratory technician, or a biology enthusiast, our 50X TAE Buffer Tris-Acetate-EDTA is a reliable and essential tool for all your nucleic acid electrophoresis experiments. Invest in the best- buy your 50X TAE Buffer today! Keywords: Tris Acetate EDTA 1L, 50X TAE Buffer 1L,50X TAE Buffer Tris Acetate EDTA, TAE Buffer, Tris Acetate, EDTA.

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Customer Questions & Answers

Question: How to Shop 50X TAE Buffer (Tris-Acetate-EDTA), 1L Online From Ubuy?

Answer: It’s easy to shop 50X TAE Buffer (Tris-Acetate-EDTA), 1L online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.
Question: Is 50X TAE Buffer (Tris-Acetate-EDTA), 1L Available to Shop Online in South Africa?

Answer: Yes, at Ubuy South Africa this product is available for you to shop at a reasonable price. The 50X TAE Buffer (Tris-Acetate-EDTA), 1L is not available locally but you can trust us with our express shipping services.
Question: How Long Does It Take to Get Product After Placing the Order?

Answer: The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

eConLabSupply Buffers Editorial Review

The 50X TAE Buffer Tris-Acetate-EDTA is a 1L solution that provides an optimal environment for nucleic acid electrophoresis. It is widely used in molecular biology labs for DNA and RNA analysis. This buffer is prepared with high-quality chemicals and has been proven to deliver consistent and reliable results. The 1L size offers great value for money and ensures that you have an ample supply of buffer for your experiments. Overall, the 50X TAE Buffer Tris-Acetate-EDTA is a highly recommended product for molecular biologists.

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Pros

Optimal environment for nucleic acid electrophoresis
High-quality chemicals
Consistent and reliable results
Great value for money
Ample supply of buffer

Product Price History

Important information

Limitations : For products shipped internationally, please note that any manufacturer warranty may not be valid; manufacturer service options may not be available; product manuals, instructions, and safety warnings may not be in destination country languages; the products (and accompanying materials) may not be designed in accordance with destination country standards, specifications, and labeling requirements; and the products may not conform to destination country voltage and other electrical standards (requiring use of an adapter or converter if appropriate). The recipient is responsible for assuring that the product can be lawfully imported to the destination country. When ordering from Ubuy or its affiliates, the recipient is the importer of record and must comply with all laws and regulations of the destination country.
Not all the products listed on Ubuy are for sale, as Ubuy is a global search engine. Products are subject to export/trade regulations.

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Order now and get it around Monday, July 06

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50X TAE Buffer (Tris-Acetate-EDTA), 1L

ZAR 1913

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What Stands Out

Product Details

Who Should Buy?

Product Description

About This Item

Customer Questions & Answers

eConLabSupply Buffers Editorial Review

Customer Reviews & Ratings

Review this product

Pros

Product Price History

Important information

ZAR 1913

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