What is Tris-HCl Buffer, pH 7.4 used for?

Tris-HCl Buffer, pH 7.4 is commonly used in molecular biology and biochemistry laboratories. It helps maintain a stable pH environment, which is crucial for biochemical reactions, enzyme activity, and RNA/DNA work. The buffer’s ability to resist pH changes makes it ideal for various applications, such as electrophoresis, chromatography, and cell culture. For instance, researchers often use it to stabilize the pH during experiments involving sensitive compounds.

How do you prepare a Tris-HCl Buffer solution?

To prepare a Tris-HCl Buffer solution, you will need Tris base and hydrochloric acid. Begin by dissolving the appropriate concentration of Tris base in distilled water, then adjust the pH to 7.4 using HCl. It's essential to measure the pH accurately to ensure the solution's effectiveness in biological experiments. This buffer is especially useful when working with proteins and nucleic acids, providing an optimal environment for stability and activity during analyses.

Is Tris-HCl Buffer suitable for use with all biological samples?

While Tris-HCl Buffer is widely used, it may not be suitable for all biological samples, especially those that are sensitive to Tris or high pH levels. Some proteins or enzymes may require different buffer systems, such as phosphate buffers. It's recommended to assess compatibility based on the specific requirements of your experiment. For example, if you're working with certain enzymes that have optimal activity under low pH, alternative buffers might be necessary.

What are the storage conditions for Tris-HCl Buffer?

Tris-HCl Buffer should be stored at room temperature, away from direct sunlight and high humidity. It is advisable to keep it in a tightly sealed container to avoid contamination and evaporation. Proper storage ensures that the buffer maintains its pH and effectiveness. In laboratory settings, it's common to have multiple prepared buffers to facilitate quick experiments without compromising their stability.

Can Tris-HCl Buffer be used in protein purification?

Yes, Tris-HCl Buffer is often utilized in protein purification protocols because of its ability to maintain a stable pH conducive to protein structure and function. This buffer is particularly important when performing techniques like ion-exchange chromatography and affinity purification, where pH can affect the binding properties of the proteins. For example, it is frequently employed in aligning the buffer conditions with those favorable for target protein isolation.

What factors influence the choice of buffer for an experiment?

Several factors influence buffer selection, including the pH range required, the biological activity of the components, and specific experimental conditions. The ionic strength, temperature stability, and compatibility with other reagents are also important considerations. Choosing Tris-HCl Buffer pH 7.4 is often preferred due to its wide applicability in various biological reactions, especially when the target molecules are stable at this pH.

Is Tris-HCl Buffer compatible with all types of assays?

While Tris-HCl Buffer is versatile and widely used, it may not be compatible with every assay, especially colorimetric assays that utilize specific indicators. The presence of Tris can interfere with certain chemical reactions or measurements. It's crucial to check assay-specific protocols before using Tris-HCl. In enzymatic assays, for instance, the buffer's ability to maintain pH can enhance accuracy but may not suit every method.

What is the significance of the pH level in Tris-HCl Buffer?

The pH level of Tris-HCl Buffer, specifically at 7.4, mimics physiological conditions, making it essential for biological experiments. Maintaining this pH helps ensure that enzymes and biomolecules function optimally, reducing the risk of denaturation or loss of activity. In cell culture and molecular biology applications, the use of this buffer helps create an environment that parallels natural conditions, which is crucial for reliable experimental outcomes.

How do you dilute Tris-HCl Buffer for specific applications?

To dilute Tris-HCl Buffer for specific applications, determine the desired final concentration and dilution factor. For instance, if you need a 0.1M solution, you can mix one part of the 1M buffer with nine parts of distilled water. Always check the final pH after dilution, as it can shift. This dilution technique is widely used in various lab protocols, ensuring proper concentration for delicate applications such as PCR and enzyme assays.

Where can I buy Tris-HCl Buffer, pH 7.4 [1M] - 1000 ml?

You can buy Tris-HCl Buffer, pH 7.4 [1M] - 1000 ml from Ubuy. Ubuy offers a vast selection of laboratory supplies and is known for its reliable service in South Africa. Whether you are stocking up for a lab or need it for specific research projects, Ubuy provides a straightforward shopping experience for purchasing essential materials like Tris-HCl Buffer.

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Item #: 97963757

Tris-HCl Buffer, pH 7.4 [1M] - 1000 ml

Item #: 97963757

ZAR 1886

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What Stands Out

High Purity

Formulated with high-quality reagents to ensure minimal contamination and reliable results, making it ideal for sensitive biological experiments.

Convenient Size

Supplied in a ready-to-use 1000 ml volume, providing ample buffer for various applications without the need for extensive preparations.

Optimal pH Stability

Maintains a consistent pH of 7.4, crucial for experiments requiring precise buffer conditions, enhancing reproducibility and accuracy in your research.

Product Details

Item Weight

2.2 lbs (1 kg)

Who Should Buy?

Molecular Biologists
Ideal for various molecular biology applications, including DNA and RNA work, ensuring stable pH conditions.
Biochemists
Useful in enzyme assays and protein purification where consistent pH is critical for reliable results.
Laboratory Technicians
A convenient buffer solution for routine lab procedures requiring neutral pH, enhancing workflow efficiency.

High Throughput Screening
Not suitable for high-throughput applications requiring minimal buffer volumes, as this product is in bulk.
Field Studies
Inapplicable for outdoor or fieldwork due to storage and stability issues; better suited for controlled environments.
Acidic Conditions
Unsuitable for experiments that require acidic pH; Tris-HCl buffers are meant for near-neutral to alkaline conditions.

Product Description

Tris-HCl Buffer, pH 7.4 [1M] - 1000 ml

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Question: How to Shop Tris-HCl Buffer, pH 7.4 [1M] - 1000 ml Online From Ubuy?

Answer: It’s easy to shop Tris-HCl Buffer, pH 7.4 [1M] - 1000 ml online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.
Question: Is Tris-HCl Buffer, pH 7.4 [1M] - 1000 ml Available to Shop Online in South Africa?

Answer: Yes, at Ubuy South Africa this product is available for you to shop at a reasonable price. The Tris-HCl Buffer, pH 7.4 [1M] - 1000 ml is not available locally but you can trust us with our express shipping services.
Question: How Long Does It Take to Get Product After Placing the Order?

Answer: The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

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Pros

High purity for reliable results
Stable pH over long periods
Versatile for various applications
Convenient 1000 ml packaging
Cost-effective for bulk use

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Occasional precipitation reported

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Limitations : For products shipped internationally, please note that any manufacturer warranty may not be valid; manufacturer service options may not be available; product manuals, instructions, and safety warnings may not be in destination country languages; the products (and accompanying materials) may not be designed in accordance with destination country standards, specifications, and labeling requirements; and the products may not conform to destination country voltage and other electrical standards (requiring use of an adapter or converter if appropriate). The recipient is responsible for assuring that the product can be lawfully imported to the destination country. When ordering from Ubuy or its affiliates, the recipient is the importer of record and must comply with all laws and regulations of the destination country.
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Features & Benefits

1M Tris-HCl Buffer, pH 7.4 available in 1000 ml.
Manufactured in ISO 9001 and 13485 certified facility in the USA.
Strict adherence to Good Manufacturing Practices and quality control tests.
Aseptic filtration through a 0.22 micron filter for purity.
Ideal for molecular biology applications including nucleic acid purification.
Complete Certificate of Analysis available for each production lot.

Tris-HCl Buffer, pH 7.4 [1M] - 1000 ml

ZAR 1886

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