What is 10X Phosphate Buffered Saline (PBS)?

10X Phosphate Buffered Saline (PBS) is a concentrated buffer solution that maintains a stable pH environment, particularly at 7.4. It is widely used in biological research, especially in cell culture and molecular biology applications. The high concentration enables researchers to dilute it to a working concentration appropriate for their experiments. This buffered saline helps maintain osmotic balance and provides essential ions needed for cellular functions.

What are the applications of 10X PBS?

10X PBS is versatile and used in various applications including cell culture, protein purification, and biochemical assays. Because it maintains the pH and ionic strength, it is ideal for diluting cells, preparing samples for western blotting, or as a wash buffer in immunoassays. For instance, a scientist might use it to resuspend cells before conducting experiments, ensuring they remain viable and functional.

How should 10X PBS be prepared for use?

To use 10X PBS, it should be diluted to a 1X working solution by mixing it with sterile distilled water in a 1:9 ratio. This means adding one part of the 10X PBS to nine parts of water. This dilution process is crucial as it creates a buffer that mimics physiological conditions, which is essential for biological studies and experiments.

Typically, 10X PBS is not inherently sterile unless specifically labeled as such. If you're using it for sensitive biological applications, it’s advisable to filter-sterilize the diluted 1X solution or purchase pre-sterilized PBS. This precaution helps prevent contamination in cell cultures or sensitive assays, ensuring that experimental results are reliable and reproducible.

Can 10X PBS be stored after dilution?

It is recommended to use the 1X PBS solution freshly prepared; however, if necessary, it can be stored at 4°C for short periods. Longer storage may lead to changes in pH or contamination. To maintain the integrity of your work, consider preparing smaller batches that can be used within a few days, thus reducing the risk of degradation or bacterial growth.

What components make up 10X PBS?

10X PBS typically consists of sodium chloride, potassium chloride, sodium phosphate, and potassium phosphate. This combination provides the necessary ionic strength and buffering capacity to stabilize the pH during biological experiments. The particular balance of these components is essential for maintaining osmotic balance, making it compatible with living cells and tissues.

How does the pH of 7.4 benefit biological experiments?

A pH of 7.4 is physiological, mimicking the natural environment of human body fluids. This property is vital for experiments involving cell cultures and enzymatic reactions as it supports optimal protein function and cellular behavior. For example, maintaining this pH is crucial when working with mammalian cells to ensure their growth and responsiveness during research.

Is there a difference between 1X PBS and 10X PBS?

Yes, the primary difference is their concentration. 10X PBS is a concentrated stock solution that needs to be diluted to achieve a 1X concentration ideal for biological work. The 10X stock allows for greater convenience in storage and long-term use, providing flexibility in preparing various volumes of dilutions suited for specific experimental needs.

Where can I buy 10X Phosphate Buffered Saline PBS, 7.4 pH, 500mL in South Africa?

You can purchase 10X Phosphate Buffered Saline PBS, 7.4 pH, 500mL from Ubuy. They offer a wide selection of laboratory products, ensuring you find the right item for your research needs. Ubuy provides a platform where you can easily browse and select necessary scientific supplies.

What safety precautions should be taken when using 10X PBS?

Standard laboratory safety precautions should be followed. Always wear protective gear, including gloves and goggles, when handling reagents. Ensure that you work in a sterile environment to minimize the risk of contamination in biological experiments. Additionally, proper labeling and storage protocols should be followed to ensure the integrity of the PBS and the safety of the user.

How to Shop 10X Phosphate Buffered Saline (PBS), 7.4 pH, 500mL Online From Ubuy?

It’s easy to shop 10X Phosphate Buffered Saline (PBS), 7.4 pH, 500mL online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.

Is 10X Phosphate Buffered Saline (PBS), 7.4 pH, 500mL Available to Shop Online in South Africa?

Yes, at Ubuy South Africa this product is available for you to shop at a reasonable price. The 10X Phosphate Buffered Saline (PBS), 7.4 pH, 500mL is not available locally but you can trust us with our express shipping services.

How Long Does It Take to Get Product After Placing the Order?

The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

eConLabSupply

10X Phosphate Buffered Saline (PBS), 7.4 pH, 500mL

10X Phosphate Buffered Saline is a balanced, isotonic salt solution essential for a variety of cell culture applications.

eConLabSupply

Item #: 127455181

10X Phosphate Buffered Saline (PBS), 7.4 pH, 500mL

Item #: 127455181

ZAR 1437

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10X Phosphate Buffered Saline is a balanced, isotonic salt solution essential for a variety of cell culture applications.

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What Stands Out

High Concentration

The 10X formulation offers a robust concentration, making it ideal for dilutions required in various biological and laboratory applications, ensuring accurate results and optimal performance for researchers.

pH Stability

Maintaining a pH of 7.4 ensures a biological compatibility that is crucial for cell culture and biochemical assays, providing researchers with a reliable buffer that mimics physiological conditions.

Bulk Volume

The 500mL packaging caters to high-demand laboratory settings, reducing the frequency of reordering and ensuring that labs have sufficient quantities for extensive studies or experiments.

Product Details

Container Type	Bottle
Item Form	Liquid
Item Weight	1 Pounds (450 grams)
Volume	500 Milliliters
Brand Name	eCon Lab Supply
Manufacturer	Ciro Mfg
Part Number	23.105.03
Included Components	Bottle
Brand	eCon Lab Supply
Item Volume	500 Milliliters
Package Information	Bottle

Who Should Buy?

Cell Biology Research
Ideal for cell cultures, maintaining optimal pH and osmotic balance for various biological experiments.
Molecular Biology Use
Recommended for applications like protein purification and cloning that require stable buffer systems.
Diagnostic Applications
Useful in laboratories for preparing samples and diluting reagents in diagnostics and assays.

Non-Laboratory Use
Not suitable for home or casual use, as it is intended for professional laboratory environments.
Sensitive Biological Applications
May not be appropriate for applications requiring ultra-pure or specialized buffering agents.
Field-Based Research
Less practical for outdoor usage where controlled environments for buffering are unavailable.

Product Description

10X Phosphate Buffered Saline (PBS), 7.4 pH, 500mL

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Question: How to Shop 10X Phosphate Buffered Saline (PBS), 7.4 pH, 500mL Online From Ubuy?

Answer: It’s easy to shop 10X Phosphate Buffered Saline (PBS), 7.4 pH, 500mL online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.
Question: Is 10X Phosphate Buffered Saline (PBS), 7.4 pH, 500mL Available to Shop Online in South Africa?

Answer: Yes, at Ubuy South Africa this product is available for you to shop at a reasonable price. The 10X Phosphate Buffered Saline (PBS), 7.4 pH, 500mL is not available locally but you can trust us with our express shipping services.
Question: How Long Does It Take to Get Product After Placing the Order?

Answer: The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

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Limitations : For products shipped internationally, please note that any manufacturer warranty may not be valid; manufacturer service options may not be available; product manuals, instructions, and safety warnings may not be in destination country languages; the products (and accompanying materials) may not be designed in accordance with destination country standards, specifications, and labeling requirements; and the products may not conform to destination country voltage and other electrical standards (requiring use of an adapter or converter if appropriate). The recipient is responsible for assuring that the product can be lawfully imported to the destination country. When ordering from Ubuy or its affiliates, the recipient is the importer of record and must comply with all laws and regulations of the destination country.
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Features & Benefits

Manufactured in a certified US facility adhering to rigorous quality control.
Contains essential components for biological research: NaCl, NaH2PO4, KCl, KH2PO4.
0.22 micron filter-sterilized for optimal purity.
Ideal for washing, transporting, and diluting cells.
Available in convenient 500 mL and 1L sizes.
Strictly for research use only, ensuring high-quality outcomes.

10X Phosphate Buffered Saline (PBS), 7.4 pH, 500mL

ZAR 1437

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Product Description

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eConLabSupply Buffers Editorial Review

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Important information

ZAR 1437

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