What is Tris-HCl Buffer, pH 8.5 [1M] used for?

Tris-HCl Buffer, pH 8.5 [1M] is a commonly used buffering agent in molecular biology and biochemistry applications. Its primary use is to maintain a stable pH in enzymatic reactions and during protein purification. The stability of pH is crucial since many biochemical reactions are sensitive to pH fluctuations, affecting enzyme activity and stability. In practical scenarios, you might use it in DNA electrophoresis to prevent pH changes during the process, ensuring effective separation.

What are the components of Tris-HCl Buffer?

Tris-HCl Buffer consists of Tris (tris(hydroxymethyl)aminomethane) and hydrochloric acid (HCl) to adjust the pH. The Tris component acts as a base, while HCl is used to lower the pH to the desired level. This combination allows for precise pH control, critical for experiments requiring specific conditions. Typically in laboratories, you will find this buffer used to create environments for DNA and RNA manipulation, ensuring optimal conditions for nucleic acid stability.

How should Tris-HCl Buffer be stored?

Tris-HCl Buffer should be stored at room temperature in a tightly sealed container to prevent contamination and evaporation. While it is stable for a reasonably long duration, periodic checks for any signs of precipitation or contamination are advisable. In laboratory settings, you will find that proper storage ensures that the buffer maintains its efficacy for consistent experimental results, particularly when used repeatedly.

Can Tris-HCl Buffer be used for protein extraction?

Yes, Tris-HCl Buffer, pH 8.5 [1M], is suitable for protein extraction. Its buffering capacity helps maintain pH during the extraction process, which is crucial as protein solubility can be affected by pH. Many researchers use this buffer for extracting proteins from different sources, as it effectively preserves their structure and function, especially for proteins that are sensitive to drastic pH changes.

Is Tris-HCl Buffer biocompatible?

Tris-HCl Buffer is generally considered biocompatible, making it suitable for use in biological applications, including cell culture and enzyme reactions. Its formulation does not introduce toxic substances that could interfere with biochemical processes. Many laboratories utilize this buffer for assays where biocompatibility is critical, ensuring cellular viability and functionality throughout experiments.

How do I prepare Tris-HCl Buffer, pH 8.5?

To prepare Tris-HCl Buffer, mix Tris base with distilled water, adjusting the pH to 8.5 by slowly adding hydrochloric acid. It’s essential to use a pH meter to ensure accuracy during the adjustment process. This preparation technique is common in research labs where precise buffer conditions are necessary for achieving reliable and repeatable results in experiments involving DNA and RNA.

Can I use Tris-HCl Buffer for gel electrophoresis?

Absolutely, Tris-HCl Buffer, pH 8.5, is often used in gel electrophoresis procedures. Its buffering capacity ensures that the pH remains stable during the separation of nucleic acids or proteins, which is essential for obtaining reproducible results. You'll find that many laboratories rely on this buffer when preparing running or casting gels, as it helps maintain the integrity of samples and supports accurate measurements.

Is Tris-HCl Buffer compatible with other chemicals?

Tris-HCl Buffer is generally compatible with a range of chemicals used in molecular biology applications. However, it’s crucial to avoid mixing it with certain dyes or agents that can react negatively at specific pH ranges. In practical terms, you should always consult compatibility charts when planning experiments. This ensures that your chosen reagents actually work together without causing unwanted reactions during your research.

What is the ideal pH range for using Tris-HCl Buffer?

The ideal pH range for using Tris-HCl Buffer is between 7.0 and 9.0, with 8.5 being a standard choice for many applications. This range is effective for most biochemical interactions involving nucleic acids and proteins. Researchers often select Tris-HCl Buffer in this pH range to ensure that they create an optimal environment for enzymatic reactions and maintain the stability of biological molecules.

Where can I buy Tris-HCl Buffer, pH 8.5 [1M] - 100mL in South Africa?

You can buy Tris-HCl Buffer, pH 8.5 [1M] - 100mL at Ubuy. Ubuy provides a comprehensive platform for purchasing laboratory reagents and equipment, ensuring you have access to high-quality buffers and chemicals required for your scientific experiments. Additionally, Ubuy often features a user-friendly interface, which makes finding and ordering the products you need straightforward.

How to Shop Tris-HCl Buffer, pH 8.5 [1M] - 100mL Online From Ubuy?

It’s easy to shop Tris-HCl Buffer, pH 8.5 [1M] - 100mL online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.

Is Tris-HCl Buffer, pH 8.5 [1M] - 100mL Available to Shop Online in South Africa?

Yes, at Ubuy South Africa this product is available for you to shop at a reasonable price. The Tris-HCl Buffer, pH 8.5 [1M] - 100mL is not available locally but you can trust us with our express shipping services.

How Long Does It Take to Get Product After Placing the Order?

The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

eConLabSupply

Produced under the highest industry standards to ensure superior results.

eConLabSupply

Item #: 94951887

Tris-HCl Buffer, pH 8.5 [1M] - 100mL

Item #: 94951887

ZAR 1751

Price Details

Excluding Shipping & Custom charges ( Shipping and custom charges will be calculated on checkout )

^{*All items will import from US}

Availability : In stock

Imported from USA store

QTY:

Order now and get it around Saturday, September 05

Secured transaction

Our Top Logistics Partners

Produced under the highest industry standards to ensure superior results.

U-Care Warranty:

None

Select a Plan

Fast
Shipping

Free
Return^*

Secure Packaging

100% Original Products

PCI DSS Compliance

ISO 27001 Certified

Note: Step Down Voltage Transformer required for using electronics products of US store (110-120). Recommended power converters Buy Now.

What Stands Out

Precise pH Control

This Tris-HCl buffer offers stable and precise pH 8.5, ensuring optimal conditions for biochemical reactions and experiments, crucial for reliable results in research and laboratory applications.

High Concentration

With a 1M concentration, this buffer provides robust buffering capacity, effectively maintaining pH levels even under varying experimental conditions, making it ideal for rigorous scientific work.

Convenient Size

Packaged in a 100mL volume, it's perfect for both small-scale experiments and larger applications, providing flexibility and ease of use for laboratories of all sizes.

Product Details

Brand	eCon Lab Supply
Item Weight	0.25 Pounds (110 grams)
Item Volume	100 Milliliters
Package Information	Bottle
Item Form	Liquid

Who Should Buy?

Molecular Biologists
Essential for DNA/RNA extraction and analysis requiring stable pH conditions in various molecular biology protocols.
Biochemists
Ideal for enzyme activity studies that require precise pH environments to ensure optimal enzymatic reactions.
Research Laboratories
Commonly used in academic and industrial research settings for buffer preparation and various biochemical assays.

Field Studies
Not suitable for outdoor or field use where temperature fluctuations can affect pH stability of the buffer.

Product Description

Tris-HCl Buffer, pH 8.5 [1M] - 100mL

Have any Query? Chat with us

Customer Questions & Answers

Question: How to Shop Tris-HCl Buffer, pH 8.5 [1M] - 100mL Online From Ubuy?

Answer: It’s easy to shop Tris-HCl Buffer, pH 8.5 [1M] - 100mL online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.
Question: Is Tris-HCl Buffer, pH 8.5 [1M] - 100mL Available to Shop Online in South Africa?

Answer: Yes, at Ubuy South Africa this product is available for you to shop at a reasonable price. The Tris-HCl Buffer, pH 8.5 [1M] - 100mL is not available locally but you can trust us with our express shipping services.
Question: How Long Does It Take to Get Product After Placing the Order?

Answer: The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

eConLabSupply Buffers Editorial Review

No editorial reviews found

Customer Reviews & Ratings

5.0

1 customers ratings

5 Star

100%
4 Star

0%
3 Star

0%
2 Star

0%
1 Star

0%

Review this product

Share your thoughts with other customers

Product Price History

Important information

Limitations : For products shipped internationally, please note that any manufacturer warranty may not be valid; manufacturer service options may not be available; product manuals, instructions, and safety warnings may not be in destination country languages; the products (and accompanying materials) may not be designed in accordance with destination country standards, specifications, and labeling requirements; and the products may not conform to destination country voltage and other electrical standards (requiring use of an adapter or converter if appropriate). The recipient is responsible for assuring that the product can be lawfully imported to the destination country. When ordering from Ubuy or its affiliates, the recipient is the importer of record and must comply with all laws and regulations of the destination country.
Not all the products listed on Ubuy are for sale, as Ubuy is a global search engine. Products are subject to export/trade regulations.

ZAR 1751

Order now and get it around Saturday, September 05

QTY:

Secured transaction

PCI DSS compliant and ISO 27001:2022 certified, with encrypted payments and full buyer protection on every order.

Our Top Logistics Partners

Fastest cross-border delivery

Features & Benefits

Comprehensive range of biological buffers for research and diagnostics.
Manufactured in an ISO 9001 and 13485 certified US facility.
Strict adherence to Good Manufacturing Practices and quality control.
Aseptically filtered solutions for optimal purity.
Complete Certificate of Analysis provided for each lot.
Ideal for applications in molecular biology and protein research.

Ubuy Assurance

Experience worry-free shopping with 100% original products, PCI DSS-compliant payment security, ISO 27001-certified data protection, the fastest cross-border delivery, free returns ^*, and secure packaging on every order.

Tris-HCl Buffer, pH 8.5 [1M] - 100mL

ZAR 1751

Fast Shipping

Free Return*

Secure Packaging

100% Original Products

PCI DSS Compliance

ISO 27001 Certified

What Stands Out

Product Details

Who Should Buy?

Product Description

Customer Questions & Answers

eConLabSupply Buffers Editorial Review

Customer Reviews & Ratings

Review this product

Product Price History

Important information

ZAR 1751

Features & Benefits

Ubuy Assurance

Fast
Shipping

Free
Return^*