What is 10X TAE Buffer Tris-Acetate-EDTA used for?

10X TAE Buffer is primarily used in molecular biology applications for agarose gel electrophoresis. It functions as a running buffer, maintaining the stability of nucleic acids during the separation process. The Tris provides a stable pH, while acetate helps in the effective separation of DNA fragments based on size. It’s an essential reagent for researchers involved in DNA analysis, cloning, and various laboratory procedures where nucleic acid handling is required.

How do you prepare 1X TAE Buffer from 10X TAE Buffer?

To prepare a 1X TAE Buffer from a 10X concentration, simply dilute it by mixing one part of 10X TAE with nine parts of distilled water. For example, if you need 100 mL of 1X TAE, combine 10 mL of 10X TAE with 90 mL of distilled water. This diluted solution is essential for various applications in gel electrophoresis, ensuring that your DNA samples are adequately buffered during the analysis process.

What are the components of 10X TAE Buffer?

10X TAE Buffer contains Tris (tris(hydroxymethyl)aminomethane), acetic acid, and EDTA (ethylenediaminetetraacetic acid). Tris acts as a buffering agent to maintain a stable pH, acetate assists in the efficient migration of nucleic acids during electrophoresis, and EDTA chelates divalent metal ions, which helps protect DNA from degradation. Each component plays a critical role in ensuring successful separation and visualization of nucleic acids during laboratory experiments.

Why is EDTA included in the 10X TAE Buffer formulation?

EDTA is included in the 10X TAE Buffer as a chelating agent. It binds to metal ions such as magnesium and calcium that could potentially catalyze the degradation of nucleic acids. This protection is crucial when handling DNA samples, allowing for clearer results during electrophoresis. Researchers often use 10X TAE in procedures that involve sensitive DNA, making EDTA a vital component of this buffer solution.

Can 10X TAE Buffer be used for RNA applications?

While 10X TAE Buffer is primarily designed for DNA applications, it can also be used for RNA applications, particularly in gel electrophoresis. The buffer's components facilitate RNA stability, but additional care should be taken to use RNase-free reagents to prevent RNA degradation. Thus, 10X TAE can be effectively employed in experiments involving RNA, making it versatile for various nucleic acid studies.

Is 10X TAE Buffer suitable for high-resolution gel electrophoresis?

Yes, 10X TAE Buffer is suitable for high-resolution gel electrophoresis and is often preferred for DNA fragment separation. The buffer's pH stability and low ionic strength allow for the precise separation of smaller DNA fragments, making it ideal for applications like DNA fingerprinting and cloning. Researchers often choose it to achieve clearer bands in their agarose gel, helping in accurate analysis and visualization.

What is the storage requirement for 10X TAE Buffer?

10X TAE Buffer should be stored at room temperature or in a refrigerator, away from light. Proper storage helps preserve the stability of its components, ensuring optimal performance during experiments. Checking for any precipitation or color changes before use is a good practice. By following these storage guidelines, researchers can ensure their buffer maintains its efficacy in future applications.

Can 10X TAE Buffer be reused after gel electrophoresis?

Generally, reusing 10X TAE Buffer after gel electrophoresis is not recommended. The buffer may become contaminated with nucleic acids or other components from the gel, which can alter its composition and affect subsequent experiments. For reliable results, it is best to use fresh buffer for each run, ensuring that sample integrity and separation accuracy are maintained throughout different experiments.

What safety precautions should be taken when handling 10X TAE Buffer?

When handling 10X TAE Buffer, it's important to wear appropriate personal protective equipment, such as gloves and goggles, to avoid contact with skin or eyes. Although the buffer is relatively safe to use, direct exposure to any chemical components could irritate. Always work in a well-ventilated area and ensure proper disposal practices for any unused buffer or waste solutions, adhering to your laboratory's safety protocols.

Where can I buy 10X TAE Buffer Tris-Acetate-EDTA, 1L in South Africa?

You can buy 10X TAE Buffer Tris-Acetate-EDTA, 1L from Ubuy, which offers a wide range of laboratory chemicals and supplies. Ubuy is known for providing quality products and can help you find the exact specifications you need for your research or educational projects, making it a reliable option for scientists and students alike.

How to Shop 10X TAE Buffer (Tris-Acetate-EDTA), 1L Online From Ubuy?

It’s easy to shop 10X TAE Buffer (Tris-Acetate-EDTA), 1L online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.

Is 10X TAE Buffer (Tris-Acetate-EDTA), 1L Available to Shop Online in South Africa?

Yes, at Ubuy South Africa this product is available for you to shop at a reasonable price. The 10X TAE Buffer (Tris-Acetate-EDTA), 1L is not available locally but you can trust us with our express shipping services.

How Long Does It Take to Get Product After Placing the Order?

The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

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Manufactured in an ISO 9001 and 13485 certified facility in the USA, our buffers guarantee unparalleled quality for your molecular biology research.

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Item #: 97962882

10X TAE Buffer (Tris-Acetate-EDTA), 1L

Item #: 97962882

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High Purity

The 10X TAE Buffer is formulated with high-purity reagents, ensuring reliable performance in nucleic acid experiments, which minimizes contamination and enhances reproducibility in research results.

Convenient Packaging

Packaged in a generous 1L volume, this buffer offers consistent performance for various applications, reducing the need for frequent repurchases and enabling extensive laboratory use without interruptions.

Versatile Applications

Ideal for gel electrophoresis and molecular biology applications, this TAE Buffer efficiently supports DNA and RNA separation, making it a crucial tool for researchers across various fields.

Product Details

Manufactured in an ISO 9001 and 13485 certified facility in the USA
For research use only. Not for human or animal clinical/therapeutic use.
Each production lot is manufactured in our USA laboratory following stringent Good Manufacturing Practices and rigorous quality control tests under certified 14644 clean room conditions
10X TAE (Tris-Acetate-EDTA) Buffer is used for running electrophoresis of nucleic acids in the gels for providing electrical conductivity and maintaining the pH. The EDTA present in the buffer inhibits metal dependent nucleases by chelating the divalent cations (Ca2+, Mg2+), and thereby protects the DNA from nucleases during the electrophoresis. The buffer may be used for both genomic and large supercoiled DNA, and also be as a gel preparation buffer.
10X Tris-EDTA Buffer (TE Buffer) is a commonly used buffer solution in molecular biology, especially in procedures involving DNA, cDNA or RNA. "TE" is derived from its components: Tris, a common pH buffer, and EDTA, a molecule that chelates cations like Mg2+. The purpose of TE buffer is to solubilize DNA or RNA, while protecting it from degradation. May also be used as a washing buffer.

Container Type	Bottle
Item Form	Gel
Item Weight	1.25 Pounds (570 grams)
Volume	1 Liters
Brand Name	eCon Lab Supply
Manufacturer	Ciro Mfg
Part Number	23.105.20
Included Components	Bottle
Brand	eCon Lab Supply
Item Volume	1 Liters
Package Information	Bottle

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Who Should Buy?

Molecular Biologists
Ideal for researchers conducting DNA analysis, gel electrophoresis, and various molecular cloning techniques.
Educational Labs
Suitable for academic institutions where students learn DNA electrophoresis and nucleic acid separation.
Genetic Research
Beneficial for labs focusing on gene sequencing and manipulation requiring reliable buffer solutions.

Non-Laboratory Users
Not suited for casual or home use where advanced molecular techniques are not conducted.
Non-DNA Applications
Ineffective for users needing buffers for RNA work or protein electrophoresis without specific adjustments.
Beginners Without Guidance
Not recommended for novices without proper training in buffer preparation and handling in molecular biology.

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Question: How to Shop 10X TAE Buffer (Tris-Acetate-EDTA), 1L Online From Ubuy?

Answer: It’s easy to shop 10X TAE Buffer (Tris-Acetate-EDTA), 1L online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.
Question: Is 10X TAE Buffer (Tris-Acetate-EDTA), 1L Available to Shop Online in South Africa?

Answer: Yes, at Ubuy South Africa this product is available for you to shop at a reasonable price. The 10X TAE Buffer (Tris-Acetate-EDTA), 1L is not available locally but you can trust us with our express shipping services.
Question: How Long Does It Take to Get Product After Placing the Order?

Answer: The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

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Pros

Highly effective buffering agent
Stable pH over time
Great for gel electrophoresis
Convenient 1L size
Trusted by labs worldwide

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May have a slight odor.

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Limitations : For products shipped internationally, please note that any manufacturer warranty may not be valid; manufacturer service options may not be available; product manuals, instructions, and safety warnings may not be in destination country languages; the products (and accompanying materials) may not be designed in accordance with destination country standards, specifications, and labeling requirements; and the products may not conform to destination country voltage and other electrical standards (requiring use of an adapter or converter if appropriate). The recipient is responsible for assuring that the product can be lawfully imported to the destination country. When ordering from Ubuy or its affiliates, the recipient is the importer of record and must comply with all laws and regulations of the destination country.
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Features & Benefits

Comprehensive range of biological buffers for research, diagnostics, and drug development.
Produced in an ISO certified facility ensuring high quality standards.
10X TAE Buffer provides optimal conditions for nucleic acid electrophoresis.
EDTA in the buffer protects DNA from nucleases during electrophoresis.
10X TE Buffer is ideal for solubilizing DNA or RNA while preventing degradation.
Buffers suitable for genomic and supercoiled DNA applications.

10X TAE Buffer (Tris-Acetate-EDTA), 1L

ZAR 1377

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