What is Phosphate Buffered Saline (PBS) Solution used for?

Phosphate Buffered Saline (PBS) Solution is primarily used in biological research and laboratories for cell culture, maintaining osmotic balance and pH stability. Its buffered nature helps keep cells alive and healthy during experiments. Researchers often utilize PBS to wash cells before assay procedures, making it essential for immunofluorescence, flow cytometry, and biochemistry applications.

How is the pH of PBS Solution significant?

The pH of PBS Solution is maintained at 7.4, which closely resembles the physiological pH of human blood. This makes it ideal for experiments involving cells as it creates an optimal environment for biological reactions. PBS at this pH helps prevent cellular stress, ensuring that experimental results reflect natural conditions, particularly in cell viability studies or drug testing scenarios.

Can I store PBS Solution at room temperature?

It is recommended to store PBS Solution at 2-8°C for optimal stability. Storing it in a cold environment helps maintain the integrity of the buffer components over time. For best practices, consider aliquoting the solution into smaller containers to avoid repeated freeze-thaw cycles, which can degrade the solution’s effectiveness in sensitive experiments such as enzyme assays or cell culture procedures.

Is the PBS Solution sterile?

Typically, commercially available PBS Solutions, especially those labeled for laboratory use, are sterile. Sterility is crucial for applications involving cell culture and microbiological tests where contamination can skew results. Before use, check for certification or indication of sterility on the packaging, ensuring that your experimental conditions remain uncontaminated in studies, such as microbial assays or genetic analysis.

What are the components of PBS Solution?

PBS Solution contains sodium chloride, sodium phosphate, and potassium phosphate, which help maintain osmolarity and a stable pH. These components are crucial for providing an isotonic environment that supports the physiological state of cells and biological materials. In practical scenarios, these ingredients make PBS suitable for diluting proteins, reconstituting lyophilized antibodies, or preparing samples for microscopy.

Can I use PBS Solution for preparing cell suspensions?

Yes, PBS Solution is ideal for preparing cell suspensions due to its isotonic nature, which prevents cells from lysing. When isolating or resuspending cells, PBS can be used as a buffer to maintain cell viability. This is especially useful in applications like flow cytometry, where maintaining cell health during analysis is crucial.

Is PBS Solution suitable for animal cell culture?

Absolutely, PBS Solution is widely used in animal cell culture as it provides necessary buffering and osmotic support. Its neutral pH ensures that cells remain healthy throughout experimental procedures. Researchers often use PBS for washing cell monolayers before adding reagents in assays involving metabolic activities or protein expression studies.

Can I use PBS Solution in molecular biology applications?

Yes, PBS Solution is frequently utilized in molecular biology for processes such as PCR and DNA extraction. Its buffering capacity ensures that enzymes function optimally, avoiding pH-induced activity loss. In practical use, PBS can be employed to wash DNA extracts or dilute samples for further analysis, ensuring high-quality results in genetic research.

Are there any alternatives to PBS Solution?

While PBS Solution is a standard choice in many laboratory protocols, alternatives like TBS (Tris-Buffered Saline) or other custom buffers may be used depending on the specific application. Each buffer has its unique properties and advantages. For example, TBS might be preferred in Western blotting due to its reduced background signal. Choosing the right buffer depends on your experimental requirements, especially in antibody applications.

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Is 1X Phosphate Buffered Saline (PBS) Solution, 7.4 pH, Available to Shop Online in South Africa?

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1X Phosphate Buffered Saline (PBS) Solution, 7.4 pH, 1 L

1X Phosphate Buffered Saline (PBS) is a balanced, isotonic salt solution used for a variety of cell culture applications

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Item #: 68749584

1X Phosphate Buffered Saline (PBS) Solution, 7.4 pH, 1 L

Q: Where can I buy 1X Phosphate Buffered Saline PBS Solution, 7.4 pH, 1 L in South Africa?

You can purchase 1X Phosphate Buffered Saline PBS Solution, 7.4 pH, 1 L from Ubuy. They offer a range of laboratory solutions and supplies, ensuring you receive quality products suitable for your research and laboratory needs.

Item #: 68749584

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1X Phosphate Buffered Saline (PBS) is a balanced, isotonic salt solution used for a variety of cell culture applications

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What Stands Out

Balanced pH

Maintains a stable pH of 7.4, ensuring optimal conditions for cellular and molecular applications, thus providing reliability for laboratory experiments and research.

Convenient Volume

Comes in a 1-liter container, perfect for various laboratory applications, reducing the need for frequent replenishing and ensuring plenty for extended usage.

High Purity

Formulated with high-quality components, this solution minimizes contamination risks, making it ideal for sensitive biological studies and improving reproducibility of results.

Product Details

Item Weight

2.2 lbs (1 kg)

Who Should Buy?

Laboratory Researchers
Ideal for researchers requiring a stable pH for cell culture and biochemical assays in laboratory settings.
Cell Biologists
Perfect for cell biologists who need a reliable medium for maintaining cell viability and physiological conditions.
Healthcare Professionals
Beneficial for healthcare specialists in diagnostics and treatment, aiding sample preparation and transport.

Field Researchers
Less suitable for field conditions where temperature control and sterility may be difficult to maintain.
Non-Scientific Uses
Not recommended for general consumer use outside laboratory or medical applications as it lacks nutritional value.
Home Experiments
May not be appropriate for amateur home experiments due to the need for proper handling and disposal.

Product Description

1X Phosphate Buffered Saline (PBS) Solution, 7.4 pH, 1 L

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Question: How to Shop 1X Phosphate Buffered Saline (PBS) Solution, 7.4 pH, Online From Ubuy?

Answer: It’s easy to shop 1X Phosphate Buffered Saline (PBS) Solution, 7.4 pH, online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.
Question: Is 1X Phosphate Buffered Saline (PBS) Solution, 7.4 pH, Available to Shop Online in South Africa?

Answer: Yes, at Ubuy South Africa this product is available for you to shop at a reasonable price. The 1X Phosphate Buffered Saline (PBS) Solution, 7.4 pH, is not available locally but you can trust us with our express shipping services.
Question: How Long Does It Take to Get Product After Placing the Order?

Answer: The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

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Pros

Maintains pH stability
High-quality formulation
Versatile for various applications
User-friendly packaging
Reliable and consistent results

Cons

Slightly bulky for some users.

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Features & Benefits

Filter-sterilized PBS with balanced isotonic salt solution
Ideal for cell culture applications
Manufactured in an ISO certified facility
For research use only
Contains 137mM NaCl, 10mM NaH2PO4, 2.7mM KCl, 1.8mM KH2PO4

1X Phosphate Buffered Saline (PBS) Solution, 7.4 pH, 1 L

ZAR 1233

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