What is a Phosphate Buffer, pH 6.5 [0.5 M] used for?

A Phosphate Buffer, pH 6.5 [0.5 M] is primarily used to maintain a stable pH in various biological and chemical experiments. This buffer is essential in contexts like enzymatic reactions, where pH can significantly affect enzyme activity and stability. In laboratory settings, it acts as a stabilizing solution for pH-sensitive processes, ensuring accurate results in procedures like DNA extraction or protein assays.

How do you prepare a Phosphate Buffer, pH 6.5?

Preparing a Phosphate Buffer at pH 6.5 involves mixing specific volumes of dihydrogen phosphate and hydrogen phosphate salts with distilled water. Typically, you'd calculate the required amounts based on the buffer's total volume. It’s important to adjust the pH accurately using a pH meter, which may involve adding small quantities of acid or base to reach the desired pH level. This process ensures that the buffer is effective for applications in various experiments.

Can the Phosphate Buffer, pH 6.5 be stored for future use?

Yes, the Phosphate Buffer, pH 6.5 can be stored for future use, typically in a sterile, closed container to prevent contamination. For optimal results, store it at room temperature or refrigerated, and ensure to check the pH before using it in experiments. This makes it convenient for labs that frequently require pH stabilization without the need to prepare fresh solutions each time.

Is the Phosphate Buffer suitable for cell culture applications?

Absolutely, the Phosphate Buffer, pH 6.5 is suitable for cell culture applications. It mimics physiological conditions, providing a stable environment that helps maintain cellular activities during experimentation. This buffer is essential when working with mammalian cells or bacteria, as fluctuations in pH can adversely affect cell behavior and metabolic functions. Thus, it’s widely used by researchers in cell biology studies.

What makes Phosphate Buffer, pH 6.5, preferable over other buffers?

The Phosphate Buffer at pH 6.5 is often preferred due to its effective buffering capacity close to physiological pH. Phosphate buffers are less likely to interfere with biological reactions compared to some organic buffers. Additionally, they are cost-effective, easy to prepare, and compatible with a wide range of biological systems. These characteristics make it an ideal choice for many laboratory applications.

What are the safety considerations for using Phosphate Buffers?

When using Phosphate Buffers, pH 6.5, typical safety precautions include wearing gloves and goggles to avoid skin and eye irritation. The chemicals involved are generally safe, but it's vital to ensure proper ventilation in the laboratory. Always refer to the Material Safety Data Sheet (MSDS) for specific handling and disposal guidelines. This ensures a safe working environment while working with scientific reagents.

Can the Phosphate Buffer be used in biochemical assays?

Yes, Phosphate Buffer, pH 6.5 is frequently used in biochemical assays to ensure optimal pH conditions for enzyme activity. Many assays, including those assessing enzymatic reactions, depend on maintaining a stable pH, and this buffer fulfills that requirement effectively. It’s an excellent choice for assays involving nucleic acids or proteins, where pH fluctuations can lead to inaccurate results.

What volume of Phosphate Buffer should I use in my experiments?

The volume of Phosphate Buffer to use in experiments varies based on specific experimental requirements. Commonly, researchers use anywhere from 5 to 50 ml, depending on the scale of the experiment and the number of samples being processed. For micro-scale experiments, smaller volumes may suffice, while larger studies may require higher volumes to maintain pH stability across samples.

Where can I buy Phosphate Buffer, pH 6.5 [0.5 M] - 250 ml in South Africa?

You can buy Phosphate Buffer, pH 6.5 [0.5 M] - 250 ml on Ubuy in South Africa. Ubuy offers a wide range of laboratory chemicals and supplies, making it a reliable option for those needing specific reagents for research or educational purposes.

Is there a difference between this buffer and other pH buffers?

Yes, there is a significant difference between Phosphate Buffer, pH 6.5 and other types of pH buffers. Each buffer has a specific pH range in which it is effective. Phosphate buffers are particularly good for biological studies due to their competence in maintaining pH stability within the physiological range. In contrast, other buffers might not perform as well in biological contexts. Choosing the right buffer ensures reliable experimental conditions.

How to Shop Phosphate Buffer, pH 6.5 [0.5 M] - 250 ml Online From Ubuy?

It’s easy to shop Phosphate Buffer, pH 6.5 [0.5 M] - 250 ml online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.

Is Phosphate Buffer, pH 6.5 [0.5 M] - 250 ml Available to Shop Online in South Africa?

Yes, at Ubuy South Africa this product is available for you to shop at a reasonable price. The Phosphate Buffer, pH 6.5 [0.5 M] - 250 ml is not available locally but you can trust us with our express shipping services.

How Long Does It Take to Get Product After Placing the Order?

The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

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Phosphate Buffer, pH 6.5 [0.5 M] - 250 ml

Manufactured in an ISO 9001 and 13485 certified facility in the USA, our biological buffers are crucial for protein research and molecular biology.

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Item #: 125268547

Phosphate Buffer, pH 6.5 [0.5 M] - 250 ml

Item #: 125268547

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What Stands Out

Precise pH Control

Maintains a stable pH of 6.5, providing accuracy in experiments and applications requiring strict pH consistency.

High Concentration

At 0.5 M, this buffer offers effective resistance to pH changes, making it suitable for sensitive biochemical reactions.

Convenient Size

The 250 ml volume is ideal for various laboratory applications, minimizing waste while maximizing usability in research and educational settings.

Product Details

Item Weight

0.55 lbs (250 grams)

Who Should Buy?

Biological Research
Ideal for experiments requiring stable pH, suitable for cellular and molecular biology applications.
Laboratory Experiments
Perfect for use in various chemistry labs that demand precise pH control during experiments.
Protein Studies
Beneficial in maintaining optimal conditions for protein purification and enzymatic reactions at pH 6.5.

High pH Applications
Not suitable for experiments requiring a pH above 7, as it is specifically formulated at pH 6.5.
DIY Solutions
Not recommended for users seeking to create custom buffer solutions due to its fixed composition.
Long-Term Storage
Not ideal for applications needing prolonged buffer stability; best used soon after opening for effectiveness.

Product Description

Phosphate Buffer, pH 6.5 [0.5 M] - 250 ml

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Question: How to Shop Phosphate Buffer, pH 6.5 [0.5 M] - 250 ml Online From Ubuy?

Answer: It’s easy to shop Phosphate Buffer, pH 6.5 [0.5 M] - 250 ml online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.
Question: Is Phosphate Buffer, pH 6.5 [0.5 M] - 250 ml Available to Shop Online in South Africa?

Answer: Yes, at Ubuy South Africa this product is available for you to shop at a reasonable price. The Phosphate Buffer, pH 6.5 [0.5 M] - 250 ml is not available locally but you can trust us with our express shipping services.
Question: How Long Does It Take to Get Product After Placing the Order?

Answer: The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

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Limitations : For products shipped internationally, please note that any manufacturer warranty may not be valid; manufacturer service options may not be available; product manuals, instructions, and safety warnings may not be in destination country languages; the products (and accompanying materials) may not be designed in accordance with destination country standards, specifications, and labeling requirements; and the products may not conform to destination country voltage and other electrical standards (requiring use of an adapter or converter if appropriate). The recipient is responsible for assuring that the product can be lawfully imported to the destination country. When ordering from Ubuy or its affiliates, the recipient is the importer of record and must comply with all laws and regulations of the destination country.
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Features & Benefits

Comprehensive range of biological buffers.
Ideal for research, diagnostics, drug development, and manufacturing.
Manufactured in an ISO 9001 and 13485 certified facility in the USA.
Stringently produced under Good Manufacturing Practices.
Quality control under certified 14644 clean room conditions.
Essential for immunochemical staining and preserving living tissues.

Phosphate Buffer, pH 6.5 [0.5 M] - 250 ml

ZAR 956

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Product Description

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Important information

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