What is Tris-HCl Buffer, pH 7.0 used for?

Tris-HCl Buffer, pH 7.0 is commonly used in biological and biochemical applications. It helps to maintain a stable pH environment that is essential for enzyme activity, DNA/RNA electrophoresis, and protein purification. The Tris buffer effectively minimizes pH fluctuations, allowing researchers to achieve more reproducible results in experiments. For example, in molecular biology experiments, a stable pH is crucial during reactions such as PCR or gel electrophoresis, where variations could lead to distorted or inaccurate results.

How do you prepare Tris-HCl Buffer at pH 7.0?

To prepare Tris-HCl Buffer at pH 7.0, you need to dissolve Tris base in distilled water and adjust the pH with hydrochloric acid (HCl) to the desired level. The buffering capacity will depend on the concentration of Tris and the final volume. For practical purposes, pre-made 1M solutions like Tris-HCl Buffer, pH 7.0, provide convenience and consistency, especially in labs where precise pH values are critical. This method is routinely applied in research facilities for applications that require consistent buffering capacity.

Can Tris-HCl Buffer be used in protein assays?

Yes, Tris-HCl Buffer, pH 7.0, is widely used in protein assays. Its neutral pH is compatible with many proteins, which ensures their stability during analysis. For instance, it is remarkable for techniques like Western blotting and ELISA, where protein stability and enzymatic activity are paramount. Using the buffer prevents denaturation and maintains the structural integrity of proteins throughout the assay process, making it a go-to solution for researchers working on enzyme-linked studies.

Is Tris-HCl Buffer suitable for electrophoresis?

Absolutely! Tris-HCl Buffer, pH 7.0, is specifically formulated for electrophoresis applications. The buffer provides optimal conditions for the migration of DNA and RNA through gel matrices during electrophoretic techniques. It also helps to maintain the stability of nucleic acids, ensuring that they do not degrade during the process. Often, labs rely on this buffer in both agarose and polyacrylamide gel electrophoresis, where a consistent pH is crucial for resolving nucleic acid fragments accurately.

What volume of Tris-HCl Buffer should I use in experiments?

The volume of Tris-HCl Buffer, pH 7.0, to use in experiments varies depending on the specific protocol you are following. Commonly, for reactions and assays, volumes of 50-200 µL are typically sufficient for small-scale experiments, while larger setups may require several milliliters. By referencing the specific requirements of your experiment, you can ensure you are using the appropriate buffer volume for optimal results, supporting consistent outcomes throughout various laboratory applications.

How should Tris-HCl Buffer be stored?

Tris-HCl Buffer, pH 7.0, should be stored at room temperature or in a cool place and kept tightly sealed to prevent contamination. It's advisable to protect it from extreme temperatures or direct sunlight. Under these conditions, the buffer can be stored for extended periods while maintaining its stability. This practice is particularly important in laboratory settings where consistent results are needed, ensuring that the buffer maintains its pH and efficacy for future experiments.

Can Tris-HCl Buffer be used for DNA or RNA storage?

Tris-HCl Buffer, pH 7.0, can indeed be used for storing DNA or RNA samples, as it helps to prevent degradation by providing a stable pH environment. Storing nucleic acids in this buffer allows for long-term preservation without compromising their integrity, making it ideal for sample management in molecular biology. This usage is especially beneficial in lab settings when preparing samples for further analysis or experimentation, where maintaining their quality is paramount.

What are the benefits of using a pre-made Tris-HCl Buffer solution?

Using a pre-made Tris-HCl Buffer, pH 7.0 streamlines laboratory processes by ensuring correct concentrations and pH values are achieved without requiring additional calculations or adjustments. This saves time and reduces the possibility of error in preparation, which is critical for experiments demanding precision. Pre-made buffers are particularly valuable in high-throughput scenarios or collaborative research, where multiple scientists rely on the same consistent buffer for their methodologies.

What are the common applications of Tris-HCl Buffer in research?

Tris-HCl Buffer, pH 7.0, is versatile and finds applications in various domains, such as molecular biology, biochemistry, and genetic research. Common uses include enzyme assays, protein purification, PCR, and nucleic acid gel electrophoresis. Its ability to maintain consistent pH makes it an essential reagent during experiments that demand precision, thus facilitating accurate interpretation of results and enhancing reproducibility across experiments or research laboratories.

Where can I buy Tris-HCl Buffer, pH 7.0 [1M] - 250mL?

You can buy Tris-HCl Buffer, pH 7.0 [1M] - 250mL from Ubuy in South Africa. Ubuy offers a convenient platform for purchasing various laboratory supplies, including specialized buffers like Tris-HCl. Their inventory ensures that you can find quality products delivered directly to your location, catering to the needs of researchers and laboratory professionals looking for reliable reagents.

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It’s easy to shop Tris-HCl Buffer, pH 7.0 [1M] - 250mL online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.

Is Tris-HCl Buffer, pH 7.0 [1M] - 250mL Available to Shop Online in South Africa?

Yes, at Ubuy South Africa this product is available for you to shop at a reasonable price. The Tris-HCl Buffer, pH 7.0 [1M] - 250mL is not available locally but you can trust us with our express shipping services.

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Tris-HCl Buffer, pH 7.0 [1M] - 250mL

Item #: 97963856

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What Stands Out

Optimal pH Stability

This Tris-HCl buffer maintains a consistent pH of 7.0, ideal for biochemical assays and molecular biology applications, ensuring reliable experimental conditions and reproducible results.

High Concentration

At 1M concentration, this buffer solution offers a robust capability to resist pH changes, making it suitable for various intricate laboratory procedures involving sensitive reactions.

Convenient Volume Size

Available in a 250mL size, this buffer is perfect for regular laboratory use, minimizing waste while providing ample quantity for multiple experiments or applications.

Product Details

Item Weight

0.55 lbs (250 grams)

Who Should Buy?

Biochemistry Students
Ideal for laboratory experiments involving enzyme reactions and protein studies at a controlled pH level.
Molecular Biology Research
Essential for applications like PCR, gel electrophoresis, and DNA/RNA manipulation requiring stable pH.
Clinical Labs
Useful in diagnostic tests that require precise buffer solutions for maintaining biological sample integrity.

Field Research
Not suitable for outdoor experiments due to stability issues and potential for buffer degradation over time.
General Household Use
Not intended for non-scientific uses; inappropriate for cooking or home applications outside laboratory settings.
High pH Applications
Inadequate for reactions or experiments needing pH levels above 7, as it specifically buffers at pH 7.0.

Product Description

Tris-HCl Buffer, pH 7.0 [1M] - 250mL

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Question: How to Shop Tris-HCl Buffer, pH 7.0 [1M] - 250mL Online From Ubuy?

Answer: It’s easy to shop Tris-HCl Buffer, pH 7.0 [1M] - 250mL online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.
Question: Is Tris-HCl Buffer, pH 7.0 [1M] - 250mL Available to Shop Online in South Africa?

Answer: Yes, at Ubuy South Africa this product is available for you to shop at a reasonable price. The Tris-HCl Buffer, pH 7.0 [1M] - 250mL is not available locally but you can trust us with our express shipping services.
Question: How Long Does It Take to Get Product After Placing the Order?

Answer: The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

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Pros

Stable pH for experiments
Easy to use
High-quality formulation
Versatile for various applications
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Bottle design could be improved.

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Limitations : For products shipped internationally, please note that any manufacturer warranty may not be valid; manufacturer service options may not be available; product manuals, instructions, and safety warnings may not be in destination country languages; the products (and accompanying materials) may not be designed in accordance with destination country standards, specifications, and labeling requirements; and the products may not conform to destination country voltage and other electrical standards (requiring use of an adapter or converter if appropriate). The recipient is responsible for assuring that the product can be lawfully imported to the destination country. When ordering from Ubuy or its affiliates, the recipient is the importer of record and must comply with all laws and regulations of the destination country.
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Features & Benefits

Comprehensive range of biological buffers for research and diagnostics.
Produced in an ISO certified facility ensuring quality.
Aseptically filtered for purity and reliability.
Suitable for protein research and molecular biology applications.
Complete Certificate of Analysis provided for each lot.
Dilutable Tris-HCl buffer for tailored concentration needs.

Tris-HCl Buffer, pH 7.0 [1M] - 250mL

ZAR 3684

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