What is DMEM Low Glucose and its purpose?

DMEM Low Glucose (Dulbecco's Modified Eagle Medium) is a nutrient-rich solution widely used in cell culture. This medium contains essential nutrients, amino acids, vitamins, and glucose in lower concentrations compared to standard DMEM. It is ideal for culturing cells that require reduced glucose concentrations, allowing for optimal growth and proliferation while minimizing the potential for increased lactate production. Researchers often use DMEM Low Glucose in studies involving sensitive cell types or metabolic research.

Can DMEM Low Glucose support various cell lines?

Yes, DMEM Low Glucose is versatile and supports a wide range of cell lines, including fibroblasts, epithelial cells, and some cancer cell lines. The medium's balanced nutrient profile promotes cell attachment, growth, and differentiation. For instance, it is commonly utilized in experiments involving primary human fibroblasts or other cells sensitive to high glucose levels, making it a go-to choice for many laboratories focusing on specific cellular responses.

How should DMEM Low Glucose be stored?

DMEM Low Glucose should be stored at 2 to 8 degrees Celsius to maintain its stability and effectiveness. It's important to avoid repeated freeze-thaw cycles which can degrade the medium's quality. For best results, aliquot the medium into smaller quantities for everyday use, minimizing the risk of contamination and degradation. Researchers should ensure that the medium is brought to room temperature before use to improve its compatibility with cells during experiments.

Is DMEM Low Glucose suitable for serum-free culture?

DMEM Low Glucose can be adapted for serum-free cultures by adding specific supplements such as growth factors or hormones tailored to the cell type in culture. This approach enables researchers to eliminate serum variables in experiments while controlling the growth environment. For example, researchers may cultivate neuronal stem cells in a serum-free formulation derived from DMEM Low Glucose to study neurogenesis under defined conditions.

What are the benefits of using Low Glucose DMEM over standard DMEM?

The primary benefit of using Low Glucose DMEM is to minimize glucose concentration, which can be critical for studying cellular metabolism and ensuring cellular responses specific to glucose levels. This medium is particularly useful for applications where high glucose concentrations might lead to toxic effects or metabolic changes, such as in cancer research or studies involving insulin signaling in adipocytes.

Which applications commonly utilize DMEM Low Glucose?

DMEM Low Glucose is widely used in various applications such as cell proliferation assays, metabolic studies, and drug response analyses. For example, in pharmacological testing, scientists may use this medium to assess the effects of new therapeutic agents on sensitive cell lines with controlled glucose levels. This adaptability makes it invaluable in both academic research and industry settings.

Are there any specific additives recommended for DMEM Low Glucose?

Yes, to enhance the performance of DMEM Low Glucose, many researchers add supplements such as fetal bovine serum (FBS), L-glutamine, or even specific growth factors. These additives can support optimal cell growth and maintenance. For instance, adding L-glutamine can help sustain cellular metabolism and improve viability, making it a common practice in many laboratories engaging in prolonged cell culture experiments.

What types of cells typically thrive in DMEM Low Glucose?

Cell types that typically thrive in DMEM Low Glucose include neuronal cells, some types of fibroblasts, and cells sensitive to high glucose conditions. For example, researchers studying neuronal differentiation often prefer this medium to ensure they observe realistic cellular behavior without the confounding effect of excessive glucose. This selection aids in precise experimental outcomes across studies on neural development and function.

Can DMEM Low Glucose be used for large-scale cell culture?

Indeed, DMEM Low Glucose is suitable for large-scale cell culture applications, providing that the cells are compatible with reduced glucose levels. For large bioprocesses, careful optimization and monitoring of nutrient levels are essential to achieve desired cell densities and product yields. This medium is commonly employed in the production of biological materials or proteins where metabolic regulation is crucial.

Where can I buy DMEM Low Glucose, 500 mL in South Africa?

You can purchase DMEM Low Glucose, 500 mL from Ubuy, an online platform known for its extensive range of scientific products and lab supplies. Ubuy offers convenient access to various laboratory reagents and materials, ensuring you can find what you need with ease. Check Ubuy for availability in South Africa and place your order for the best quality DMEM Low Glucose.

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The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

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Dulbecco's Modified Eagle Medium: the ultimate cell culture solution for a wide range of cell types.

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Item #: 174359097

DMEM Low Glucose, 500 mL

Item #: 174359097

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What Stands Out

Low Glucose Formula

Designed specifically for culturing cells that require reduced glucose concentration, supporting optimal growth and functionality in sensitive cell lines.

Ideal Volume

The 500 mL packaging provides sufficient media for extensive experiments, reducing waste and ensuring ample supply for multiple cell culture applications.

Versatile Application

Suitable for a wide range of cell types, this medium adapts to various research needs, making it a valuable asset for laboratories and biotech industries.

Product Details

Item Form	Liquid
Item Weight	1.5 Pounds (680 grams)
Volume	500 Milliliters
Brand Name	ABI
Manufacturer	American BioInnovations
Part Number	ABI-105-03
Brand	ABI
Item Volume	500 Milliliters

Who Should Buy?

Cell Culture Labs
Ideal for laboratories focusing on culturing cells that require a low glucose environment for optimal growth.
Mammalian Studies
Suitable for research involving mammalian cells, especially when studying glucose-sensitive pathways and metabolism.
Biotechnology Research
Useful for biotechnologists working with recombinant cells that thrive in low glucose conditions for protein production.

High Glucose Needs
Not suitable for cell types or experiments that require high glucose concentrations for successful growth and metabolism.
Microbial Culturing
Unsuitable for microbial applications, as many bacteria require higher glucose levels for optimal growth and replication.
Continuous Cultures
Not recommended for continuous culture systems where high glucose levels are necessary to maintain cell viability and proliferation.

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DMEM Low Glucose, 500 mL

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Question: How to Shop DMEM Low Glucose, 500 mL Online From Ubuy?

Answer: It’s easy to shop DMEM Low Glucose, 500 mL online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.
Question: Is DMEM Low Glucose, 500 mL Available to Shop Online in South Africa?

Answer: Yes, at Ubuy South Africa this product is available for you to shop at a reasonable price. The DMEM Low Glucose, 500 mL is not available locally but you can trust us with our express shipping services.
Question: How Long Does It Take to Get Product After Placing the Order?

Answer: The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

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Pros

Commonly used media
Versatile for various applications
Compatible with many cell types

Cons

May cause inconsistent results.

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Features & Benefits

High Glucose DMEM is ideal for diverse cell types including human, mouse, and fish.
Supports growth of critical cell lines such as HeLa and 293.
Custom bulk formulations available to meet specific culture needs.
Cell Culture tested with key supplements like fetal bovine serum.
Ultrapure and sterile for optimal laboratory standards.
Features Phenol Red and Pyruvate for enhanced performance.

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DMEM Low Glucose, 500 mL

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