What is the primary use of bioWORLD DTT DL-Dithiothreitol?

bioWORLD DTT DL-Dithiothreitol is primarily used as a reducing agent in biochemical and molecular biology applications. It is crucial for maintaining proteins in their reduced state, thus preventing oxidative damage during experiments. DTT helps in the disruption of disulfide bonds, which is essential when working with proteins to ensure they retain their active conformation. In practical scenarios, it's widely used in protein studies, such as enzyme assays, and in sample preparation for SDS-PAGE gel electrophoresis.

Is bioWORLD DTT DL-Dithiothreitol safe to use in laboratory experiments?

Yes, bioWORLD DTT DL-Dithiothreitol is generally safe to use under standard laboratory precautions. However, it is essential to handle it in a well-ventilated area while wearing gloves and goggles, as it can be irritating to the skin, eyes, and respiratory system. Proper personal protective equipment is crucial. In real-world applications, laboratory professionals commonly use it in protein purification protocols without issues, provided that they adhere to safety guidelines.

How should bioWORLD DTT DL-Dithiothreitol be stored?

bioWORLD DTT DL-Dithiothreitol should be stored in a cool, dry place, ideally at -20°C to maintain its stability over time. It’s recommended to keep it in its original container, tightly sealed, and away from light. This proper storage helps to preserve its reducing properties, ensuring consistent performance in experiments. Users often aliquot it to avoid repeated freeze-thaw cycles which can degrade the compound, making it ideal for long-term storage.

Can bioWORLD DTT DL-Dithiothreitol be used in cell culture applications?

Yes, bioWORLD DTT DL-Dithiothreitol can be used in certain cell culture applications where maintaining a reduced environment is required. Specifically, it is employed to prevent oxidative stress in cell cultures, particularly when working with sensitive cell types. In practice, DTT is added in small concentrations to media or buffer solutions to enhance cell viability and function, especially during transfection or when processing fragile cell lines.

What is the molecular weight of bioWORLD DTT DL-Dithiothreitol?

The molecular weight of bioWORLD DTT DL-Dithiothreitol is approximately 154.25 g/mol. Knowing the molecular weight is important for researchers to accurately prepare dilutions and concentrations for their experiments. In lab scenarios, scientists often need to calculate molar concentrations for precise reactions; hence, having this information readily available aids in experimental planning and execution.

Does bioWORLD DTT DL-Dithiothreitol affect protein function?

bioWORLD DTT DL-Dithiothreitol is designed to aid in preserving protein function by reducing disulfide bonds, which can lead to denaturation if left intact. By maintaining proteins in their reduced form, DTT ensures that enzymes and other proteins retain their active conformation crucial for biochemical reactions. For instance, in enzyme activity assays, using DTT can enhance the accuracy of results as it reflects the natural state of proteins in vivo.

How is bioWORLD DTT DL-Dithiothreitol typically used in laboratory protocols?

bioWORLD DTT DL-Dithiothreitol is typically used in various laboratory protocols, including protein purification, gel electrophoresis, and Western blotting. In these applications, it helps to prevent protein aggregation and maintains proteins in a reduced state. For instance, during SDS-PAGE, adding DTT to the sample buffer allows proteins to be fully denatured, which aids in accurate size determination during electrophoresis.

Can bioWORLD DTT DL-Dithiothreitol be used in combination with other reagents?

Yes, bioWORLD DTT DL-Dithiothreitol can often be used in combination with other reagents, such as protease inhibitors and chaotropic agents. This synergistic usage enhances overall experimental outcomes. For example, when purifying proteins, DTT is frequently paired with protease inhibitors to safeguard proteins from degradation while also maintaining their reduced state, ensuring more reliable experimental results.

What is the shelf life of bioWORLD DTT DL-Dithiothreitol once opened?

The shelf life of bioWORLD DTT DL-Dithiothreitol, once opened and stored properly, is typically up to 12 months. It's crucial to maintain recommended storage conditions, including keeping it tightly sealed and minimizing exposure to air and moisture. In practice, researchers often label containers with the date of opening to track usage and replace the product when its shelf life expires, ensuring consistently reliable performance in experiments.

Where can I buy bioWORLD DTT DL-Dithiothreitol, 100 Grams in South Africa?

You can buy bioWORLD DTT DL-Dithiothreitol, 100 Grams, from Ubuy. They offer a wide selection of laboratory products, including DTT, with convenient shopping options tailored for customers in South Africa. Ubuy's platform provides detailed product information and user reviews, ensuring informed purchasing decisions.

How to Shop bioWORLD DTT (DL-Dithiothreitol), 100 Grams Online From Ubuy?

It’s easy to shop bioWORLD DTT (DL-Dithiothreitol), 100 Grams online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.

Is bioWORLD DTT (DL-Dithiothreitol), 100 Grams Available to Shop Online in South Africa?

Yes, at Ubuy South Africa this product is available for you to shop at a reasonable price. The bioWORLD DTT (DL-Dithiothreitol), 100 Grams is not available locally but you can trust us with our express shipping services.

How Long Does It Take to Get Product After Placing the Order?

The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

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bioWorld's DTT is tested for proteomics research, sealed under Nitrogen, and assured of quality.

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Item #: 25095595

bioWORLD DTT (DL-Dithiothreitol), 100 Grams

Item #: 25095595

ZAR 16985

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What Stands Out

High Purity

bioWORLD DTT offers exceptional purity, making it ideal for sensitive biological applications where contaminants may affect results, ensuring reliable and reproducible outcomes for researchers.

Reliable Reductant

Acting as a powerful reducing agent, DTT effectively stabilizes proteins and prevents oxidation, enhancing the integrity of biological samples in lab experiments and analyses.

Convenient Quantity

Available in a generous 100-gram package, this product provides great value for laboratories of all sizes, ensuring ample supply for frequent use in various experimental protocols and applications.

Product Details

100 grams of biotechnology grade DTT DL-Dithiothreitol
SH Content: >99.4%, CAS# 3483-12-3 (also identified as CAS# 27565-41-9)
Melting range: 39-43°C, Solubility (5%, water): Clear and haze-free
Used to reduce disulfide linkages while maintaining thiol group in the reduced state in solution
Tested for proteomics research, sealed under Nitrogen, and assured of quality
Synonyms: Threitol, 1,4-dithio-,dl-; 2,3-butanediol, 1,4-dimercapto dl-threo; Cleland's Reagent

Item Weight

0.22 lbs (100 grams)

Who Should Buy?

Biochemists and Researchers
Ideal for those conducting protein stabilization or enzymatic assays requiring reducing agents in their experiments.
Molecular Biologists
Useful for protocols involving DNA manipulation and RNA extraction, where reduced disulfide bonds are critical.
Laboratory Technicians
Suitable for technicians involved in sample preparation, especially in studies involving thermal stability assessments.

Non-Scientific Users
Not suitable for casual users or individuals without training in handling biochemical reagents safely.
Home Use
Inappropriate for at-home applications due to specific storage and handling requirements of DTT.
Inexperienced Students
May pose challenges for students lacking experience in laboratory procedures involving reducing agents.

Product Description

bioWORLD DTT (DL-Dithiothreitol), 100 Grams

About This Item

Looking for a high-quality reducing agent for your research needs? Look no further than bioWORLD's DTT DL-Dithiothreitol! This 100-gram package of DTT is perfect for use in reducing disulfide linkages while maintaining the thiol group in the reduced state in your solutions. Unlike many other DTT preparations, bioWORLD's DTT is tested for proteomics research, sealed under nitrogen, and assured of quality. Say goodbye to unreliable, oxidized DTT and hello to a more consistent and accurate product.

Our DTT DL-Dithiothreitol is also known by several other names, including Threitol and 1,4-dithio, dl-, making it a versatile choice for a variety of research applications. Whether you're working in molecular biology, biochemistry, enzymology, or any other field that requires reliable DTT disulfide reduction, bioWORLD's DTT is the perfect choice. Buy bioWORLD DTT for research with confidence, knowing that you're getting a high-quality product that won't let you down.

From protein analysis to cell lysis to antibody purification and beyond, bioWORLD's DTT is the reducing agent you can trust. Order now and experience the bioWORLD difference!.

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Customer Questions & Answers

Question: How to Shop bioWORLD DTT (DL-Dithiothreitol), 100 Grams Online From Ubuy?

Answer: It’s easy to shop bioWORLD DTT (DL-Dithiothreitol), 100 Grams online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.
Question: Is bioWORLD DTT (DL-Dithiothreitol), 100 Grams Available to Shop Online in South Africa?

Answer: Yes, at Ubuy South Africa this product is available for you to shop at a reasonable price. The bioWORLD DTT (DL-Dithiothreitol), 100 Grams is not available locally but you can trust us with our express shipping services.
Question: How Long Does It Take to Get Product After Placing the Order?

Answer: The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

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Pros

Highly effective reducing agent
Stabilizes protein structures
Easy to use in experiments
Comes in convenient 100-gram size
Excellent shelf life

Cons

Strong odor during use

Product Price History

Important information

Limitations : For products shipped internationally, please note that any manufacturer warranty may not be valid; manufacturer service options may not be available; product manuals, instructions, and safety warnings may not be in destination country languages; the products (and accompanying materials) may not be designed in accordance with destination country standards, specifications, and labeling requirements; and the products may not conform to destination country voltage and other electrical standards (requiring use of an adapter or converter if appropriate). The recipient is responsible for assuring that the product can be lawfully imported to the destination country. When ordering from Ubuy or its affiliates, the recipient is the importer of record and must comply with all laws and regulations of the destination country.
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ZAR 16985

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Features & Benefits

DTT is used to reduce disulfide linkages while maintaining the thiol group in the reduced state in solution.
Contains >99.4% SH Content
Synonyms: Threitol, 1,4-dithio-,dl-; 2,3-butanediol, 1,4-dimercapto dl-threo; Cleland's Reagent.
CAS# 3483-12-3 (also identified as CAS# 27565-41-9)
Melting range: 39-43°C
Solubility (5%, water): Clear and haze-free

bioWORLD DTT (DL-Dithiothreitol), 100 Grams

ZAR 16985

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100% Original Products

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What Stands Out

Product Details

Who Should Buy?

Product Description

About This Item

Customer Questions & Answers

Bioworld Analytical Reagents Editorial Review

Customer Reviews & Ratings

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Pros

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Product Price History

Important information

ZAR 16985

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