What is the primary use of STET Solution 8% Sucrose, 5% Triton X-100, 50mM EDTA, 50mM Tris.HCl?

STET Solution is primarily used for the extraction and purification of nucleic acids and proteins. The combination of 8% sucrose and 5% Triton X-100 helps in lysing cellular membranes effectively, while EDTA chelates divalent metal ions that could inhibit enzymatic reactions. This makes it ideal for molecular biology applications, including DNA and RNA preparations in research laboratories.

Why is pH 8.0 important in STET Solution?

A pH of 8.0 is crucial for optimal enzymatic activity and stability of nucleic acids. At this pH, many enzymes, such as DNA polymerases, function at their best, ensuring higher yields of isolated DNA and RNA. This pH also helps maintain the integrity of the biological molecules throughout the extraction process, which is important for downstream applications like PCR and cloning.

What are the components of STET Solution and their functions?

STET Solution contains 8% sucrose, which helps protect biomolecules during lysis, and 5% Triton X-100, a non-ionic detergent that disrupts cell membranes. Additionally, it includes 50mM EDTA to bind metal ions that can affect enzymatic reactions and 50mM Tris.HCl to maintain stable pH. Together, these components facilitate the effective extraction of nucleic acids and proteins for various analytical procedures.

Can STET Solution be used with various types of cells?

Yes, STET Solution is versatile and can be used with a wide range of cell types, including bacterial, yeast, and mammalian cells. The composition effectively disrupts cellular structures, allowing for the extraction of internal components. This is particularly useful in genetic research and protein studies, where the type of cell can vary significantly depending on the experimental design.

What precautions should be taken when using STET Solution?

When using STET Solution, it is important to handle reagents in a well-ventilated area and wear appropriate personal protective equipment, including gloves and goggles. Avoiding exposure to light is also advised as some components may be sensitive. Additionally, proper storage of the solution at recommended conditions can prevent degradation of its components, ensuring optimal performance during your experimental procedures.

Is STET Solution suitable for long-term storage?

STET Solution can be stored for an extended period if kept at the recommended temperature and protected from light. Typically, it is advisable to store the solution at 4°C to maintain its integrity and effectiveness. However, it’s essential to monitor for any signs of precipitation or changes in color which could indicate degradation, rendering the solution less effective for future experiments.

Can I use STET Solution for both DNA and RNA extractions?

Yes, STET Solution is effective for extracting both DNA and RNA. Its formulation is designed to break down cellular membranes while protecting nucleic acids from degradation. This dual capability makes it a valuable tool in molecular biology labs for various applications, including cloning, sequencing, and gene expression analysis.

What specific applications can benefit from using STET Solution?

Applications such as plasmid DNA isolation, RNA extraction, and protein purification benefit significantly from the use of STET Solution. Its effective lysis properties and protection of nucleic acids make it ideal for techniques such as PCR, gel electrophoresis, and transfection studies, ensuring high-quality results in molecular biology experiments.

How can I ensure the effectiveness of STET Solution during use?

To ensure the effectiveness of STET Solution, make sure to follow the recommended protocols for your specific application. Buffer preparation should be done with high-quality reagents to avoid contamination. Use proper pipetting techniques to prevent introducing air bubbles which can affect results, and always work with freshly prepared samples when possible to maximize yield and quality.

How to Shop STET Solution 8% Sucrose, 5% Triton X-100, 50mM Online From Ubuy?

It’s easy to shop STET Solution 8% Sucrose, 5% Triton X-100, 50mM online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.

Is STET Solution 8% Sucrose, 5% Triton X-100, 50mM Available to Shop Online in South Africa?

Yes, at Ubuy South Africa this product is available for you to shop at a reasonable price. The STET Solution 8% Sucrose, 5% Triton X-100, 50mM is not available locally but you can trust us with our express shipping services.

How Long Does It Take to Get Product After Placing the Order?

The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

CephamLifeSciences

Item #: 54005981

STET Solution 8% Sucrose, 5% Triton X-100, 50mM EDTA, 50mM Tris.HCl, pH 8.0 - 500 ml -from Cepham Life Sciences

Q: Where can I buy STET Solution 8% Sucrose, 5% Triton X-100, 50mM EDTA, 50mM Tris.HCl, pH 8.0 - 500 ml from Cepham Life Sciences?

You can buy STET Solution from Ubuy in South Africa. Ubuy offers a user-friendly platform for purchasing various laboratory supplies, including biochemicals like STET Solution from reputable brands like Cepham Life Sciences, ensuring you have access to quality products for your research needs.

Item #: 54005981

ZAR 2229

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What Stands Out

Optimized Composition

Formulated with 8% sucrose and 5% Triton X-100 to enhance solubilization, ensuring efficient extraction and preservation of sensitive biomolecules.

Buffer Stability

Contains 50mM EDTA and 50mM Tris.HCl pH 8.0, providing a stable environment for enzymatic reactions and preventing metal ion interference.

Volume Convenience

Available in a convenient 500 ml size, making it easy to handle for lab applications, reducing the frequency of reordering and downtime in experiments.

Product Details

Item Weight

1.1 lbs (500 grams)

Who Should Buy?

Molecular Biology
Ideal for experiments requiring membrane disruption and protein extraction using detergents like Triton X-100.
Cell Biology
Useful for cell lysis applications where maintaining cellular components is essential during extraction processes.
Biochemical Research
Suitable for researchers looking to study proteins in solution and conduct enzymatic assays involving EDTA.

General Use
Not suitable for applications where non-ionic detergents may interfere with protein function or activity.
Sensitive Assays
Not recommended for protocols requiring high protein purity as Triton X-100 can denature proteins.
Living Cells
Inappropriate for use directly on living cells due to lytic properties of both Triton X-100 and EDTA.

Product Description

STET Solution 8% Sucrose, 5% Triton X-100, 50mM EDTA, 50mM Tris.HCl, pH 8.0 - 500 ml -from Cepham Life Sciences

About This Item

Introducing the Cepham Life Sciences STET Solution (8% Sucrose, 5% Triton X-100, 50mM EDTA, 50mM Tris.HCl, pH 8.0) - a versatile buffer specifically designed for DNA/RNA extraction and purification in biomolecular procedures. Designed by Cepham Life Sciences, a trusted name in the field of scientific research, this STET Solution ensures reliable and consistent results in your experiments. Why choose our STET Solution? Let's break it down: 1. High-Quality Ingredients: This solution is formulated with a precise combination of 8% sucrose, 5% Triton X-100, 50mM EDTA, and 50mM Tris.HCl, pH 8.0. Each component is carefully selected to guarantee optimal performance and purity. 2.

DNA/RNA Extraction and Purification: Whether you're working on PCR, genotyping, or gene expression studies, this STET Solution is an essential tool. It efficiently lyses cells, releasing nucleic acids and ensuring minimal degradation, making it perfect for downstream applications. 3. Research Use Only: Please note that this product is intended for research purposes only.

It is not suitable for human or animal clinical/therapeutic use. Always follow safety instructions and handle it with care. With a generous volume of 500ml, this STET Solution provides you with ample supply for multiple experiments, reducing the need for frequent reordering. Overall, Cepham Life Sciences STET Solution is a reliable and convenient solution for your DNA/RNA extraction and purification needs. Trust in our expertise and equip yourself with the best tools for your research journey.

Order now and unlock new possibilities in your laboratory procedures.

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Customer Questions & Answers

Question: How to Shop STET Solution 8% Sucrose, 5% Triton X-100, 50mM Online From Ubuy?

Answer: It’s easy to shop STET Solution 8% Sucrose, 5% Triton X-100, 50mM online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.
Question: Is STET Solution 8% Sucrose, 5% Triton X-100, 50mM Available to Shop Online in South Africa?

Answer: Yes, at Ubuy South Africa this product is available for you to shop at a reasonable price. The STET Solution 8% Sucrose, 5% Triton X-100, 50mM is not available locally but you can trust us with our express shipping services.
Question: How Long Does It Take to Get Product After Placing the Order?

Answer: The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

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Pros

Effective for cell lysis
High-quality ingredients
Ease of use
consistent results
Widely recommended by experts

Cons

The bottle design could be improved.

Product Price History

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STET Solution 8% Sucrose, 5% Triton X-100, 50mM EDTA, 50mM Tris.HCl, pH 8.0 - 500 ml -from Cepham Life Sciences

ZAR 2229

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About This Item

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CephamLifeSciences Aqueous Solutions Editorial Review

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ZAR 2229

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