What is Phosphate Buffered Saline EDTA used for?

Phosphate Buffered Saline EDTA, commonly known as PBS-EDTA, serves multiple purposes in biological laboratories. It is primarily used for cell culture applications, providing a stable environment for cells. The phosphate buffer maintains physiological pH while EDTA acts as a chelating agent, sequestering divalent cations that can interfere with enzymatic reactions. This makes PBS-EDTA ideal for processes like cell washing, immunostaining, and tissue preservation, ensuring cellular integrity during experiments.

Is PBS-EDTA sterile, and why is it important?

Yes, the PBS-EDTA solution is sterilized during production, which is critical for maintaining cell viability in laboratory settings. Sterility prevents microbial contamination that could compromise experimental results, particularly in cell culture, where bacteria or fungi could alter growth conditions and outcomes. By using sterile PBS-EDTA, you ensure that your cells or samples are handled in a controlled environment, reducing the risk of unwanted variables in your research.

How should Phosphate Buffered Saline EDTA be stored?

Phosphate Buffered Saline EDTA should be stored at room temperature, away from direct sunlight and extreme temperatures. Proper storage is essential to maintain its efficacy and sterility. Ensure that the bottle is tightly sealed and check for expiration dates before usage. Storing it correctly ensures that the PBS-EDTA will remain suitable for cellular and biochemical applications, enabling reliable results in your experiments.

Can Phosphate Buffered Saline EDTA be used in DNA extraction?

Yes, PBS-EDTA is frequently utilized in DNA extraction protocols. The EDTA component chelates magnesium ions, inhibiting enzymes like DNases that may degrade DNA. This action preserves the integrity of the DNA during isolation processes. Researchers often choose PBS-EDTA when working with sensitive samples, ensuring a higher purity of extracted DNA for downstream applications, including cloning and sequencing.

What is the importance of using PBS-EDTA in flow cytometry?

PBS-EDTA is essential in flow cytometry for maintaining cell integrity and enabling accurate analysis. The EDTA helps to prevent cell aggregation by chelating calcium ions, which are essential for cell adhesion. By using PBS-EDTA, researchers can obtain single-cell suspensions that facilitate precise measurements of cell characteristics, such as size and granularity, enhancing the reliability of flow cytometry results.

Can I use PBS-EDTA on sensitive cell types?

PBS-EDTA is suitable for many sensitive cell types, including primary cells and stem cells, due to its gentle composition. It maintains a stable pH and minimizes stress on cells, which is vital for preserving their functionality. Researchers often prefer PBS-EDTA for washing or resuspending sensitive cells to minimize damage while ensuring a prompt and effective cell handling process.

What are the components of Phosphate Buffered Saline EDTA?

Phosphate Buffered Saline EDTA consists of two primary components: phosphate buffer and ethylenediaminetetraacetic acid (EDTA). The phosphate buffer comprises sodium phosphate and potassium phosphate, enabling the solution to maintain a neutral pH. The EDTA acts as a chelating agent, binding to metal ions that could be detrimental to biological reactions. This formulation makes PBS-EDTA versatile for various laboratory applications, including cell maintenance and biochemical assays.

Is there any special preparation needed before using PBS-EDTA?

Typically, PBS-EDTA is ready to use straight from the bottle without additional preparation. However, it is advisable to gently mix the solution if there is any visible sediment. Before application, especially in sensitive experiments, checking the sterility of the liquid is crucial to avoid introducing contaminants. Proper handling guidelines ensure optimal results in cell cultures and biochemical assays.

Where can I buy Phosphate Buffered Saline EDTA, 1 X PBS-EDTA, Sterile, 500 mL?

You can buy Phosphate Buffered Saline EDTA, 1 X PBS-EDTA, Sterile, 500 mL from Ubuy in South Africa. Ubuy provides a reliable platform for purchasing laboratory supplies and ensures that you have access to high-quality products for your scientific needs.

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Is Phosphate Buffered Saline EDTA, 1 X PBS-EDTA, Available to Shop Online in South Africa?

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ABI

Phosphate Buffered Saline EDTA, 1 X PBS-EDTA, Sterile, 500 mL

A versatile buffer commonly used in procedures for generating dendritic cells from monocytes and in animal embryo transfer.

ABI

Item #: 68232413

Phosphate Buffered Saline EDTA, 1 X PBS-EDTA, Sterile, 500 mL

89% of respondents would recommend this to a friend

Item #: 68232413

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A versatile buffer commonly used in procedures for generating dendritic cells from monocytes and in animal embryo transfer.

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What Stands Out

Sterile Solution

Each 500 mL bottle of PBS-EDTA is sterilized to ensure no contamination, providing a reliable medium for biological and laboratory applications.

Enhanced Buffering

The phosphate buffer maintains a stable pH, crucial for preserving cell viability and function during experiments, making it superior to competing products.

Convenient Packaging

Offered in a ready-to-use 500 mL volume, this PBS-EDTA solution reduces preparation time in the lab, catering to both small and large-scale research needs.

Product Details

Container Type	Bottle
Item Form	Liquid
Item Weight	1.5 pounds (680 grams)
Volume	500 ml
Brand Name	ABI
Manufacturer	American BioInnovations
Part Number	ABI-102-05
Brand	ABI
Item Volume	500 ml
Package Information	Bottle

Who Should Buy?

Cell Culture
Ideal for maintaining pH levels and osmolality in cell culture applications, ensuring optimal cell viability and growth.
Biological Sample Storage
Suitable for preserving biological samples, as EDTA chelates metal ions, preventing degradation and maintaining sample integrity.
Molecular Biology Applications
Commonly used in buffer solutions for DNA extraction and manipulation, providing a stable environment for enzymatic reactions.

High-Volume Uses
Not suitable for large-scale applications where bulk solutions are required, as it comes in a 500 mL bottle.

Product Description

Phosphate Buffered Saline EDTA, 1 X PBS-EDTA, Sterile, 500 mL

About This Item

Looking for a high-quality solution for your buffer preparation needs? Look no further than our Phosphate Buffered Saline EDTA. This sterile solution is perfect for procedures such as generating dendritic cells from monocytes and animal embryo transfers. Our 1 X PBS-EDTA solution is perfect for use in cell culture and tissue culture procedures. With EDTA included in the buffered saline solution, you can rest assured that your research is in good hands. Our PBS-EDTA solution is also affordably priced, making it a top choice for researchers on a budget.

Plus, with our bulk PBS-EDTA solution available for sale, you can stock up and save even more. Don't settle for less when it comes to your research. Choose the best with our high-quality Phosphate Buffered Saline EDTA solution. Order online today and experience the difference for yourself!.

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Question: How to Shop Phosphate Buffered Saline EDTA, 1 X PBS-EDTA, Online From Ubuy?

Answer: It’s easy to shop Phosphate Buffered Saline EDTA, 1 X PBS-EDTA, online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.
Question: Is Phosphate Buffered Saline EDTA, 1 X PBS-EDTA, Available to Shop Online in South Africa?

Answer: Yes, at Ubuy South Africa this product is available for you to shop at a reasonable price. The Phosphate Buffered Saline EDTA, 1 X PBS-EDTA, is not available locally but you can trust us with our express shipping services.
Question: How Long Does It Take to Get Product After Placing the Order?

Answer: The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

ABI Buffers Editorial Review

The Phosphate Buffered Saline EDTA offers a sterile solution in a convenient 500 mL size. It is a reliable and efficient product for various laboratory applications. The pH is well-balanced and maintains stability, ensuring accurate and reproducible results. The sterile packaging enhances safety and enables immediate usage without additional sterilization procedures. Overall, the Phosphate Buffered Saline EDTA is a highly recommended product for laboratory use.

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Pros

Sterile solution
Convenient 500 mL size
Well-balanced pH
Reliable and efficient
Safe to use
Immediate usage without additional sterilization

Product Price History

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Phosphate Buffered Saline EDTA, 1 X PBS-EDTA, Sterile, 500 mL

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Product Description

About This Item

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