What is 50X TAE buffer used for in molecular biology?

50X TAE buffer is commonly used in molecular biology applications, particularly for agarose gel electrophoresis. It provides a conductive medium for DNA and RNA analysis. The buffer helps maintain pH and offers a stable environment, which is crucial for the integrity of nucleic acids during electrophoresis. Many researchers utilize 50X TAE for separating large DNA fragments efficiently, making it suitable in applications like DNA cloning, restriction enzyme assays, and DNA fingerprinting.

How should 50X TAE buffer be prepared for use?

To prepare 1X TAE buffer from a 50X stock solution, dilute it with distilled water. For example, mix 20 mL of 50X TAE with 980 mL of distilled water to create 1 liter of 1X TAE buffer. This dilution brings the concentration to the desired 1X level suitable for running gels. Ensuring proper dilution is key to achieving the best results in experiments involving gel electrophoresis.

Can 50X TAE buffer be reused after electrophoresis?

Yes, 50X TAE buffer can be reused after electrophoresis, but with caution. It is advisable to filter the buffer to remove any potential contaminants, such as dyes and DNA fragments. Reusing buffer can save costs and reduce waste, as long as it remains clear and free of contamination. Researchers often utilize reused buffer for initial runs before replacing it with fresh solution for final assessments.

What are the advantages of using TAE buffer over other buffers?

TAE buffer offers several advantages, particularly in DNA applications, such as lower ionic strength, which reduces the risk of DNA fragmentation during electrophoresis. It also provides superior resolution for larger DNA fragments compared to other buffers like TBE. Researchers find TAE particularly useful in handling samples such as plasmid DNA due to its compatibility with a variety of downstream applications, including cloning and sequencing.

How long can 50X TAE buffer be stored?

50X TAE buffer can be stored for an extended period when kept in a cool, dark place, preferably at room temperature or in a refrigerator. If the buffer is stored correctly, it can be viable for several months without significant degradation. However, always check for any signs of contamination or precipitation before use, ensuring optimal performance during experiments.

Is 50X TAE buffer suitable for RNA electrophoresis?

Yes, 50X TAE buffer is suitable for RNA electrophoresis. Its components maintain a stable pH essential for RNA stability during gel running. Researchers frequently use TAE for analyzing RNA samples, especially for techniques such as Northern blotting. Utilizing TAE buffer ensures good separation and resolution of RNA molecules, facilitating accurate analysis and interpretation of results.

Can 50X TAE buffer be used for protein electrophoresis?

While 50X TAE buffer is primarily designed for nucleic acid work, it can also be used for protein electrophoresis, although it is not the most common choice. Its low ionic strength may not provide optimal resolution for proteins compared to buffers like SDS-PAGE. In specific contexts where nucleic acids and proteins are analyzed together, TAE can be utilized, though proteins typically benefit more from dedicated protein running buffers.

What is the pH of 50X TAE buffer?

The pH of 50X TAE buffer is typically around 8.0. This alkaline pH is crucial to stabilize nucleic acids during electrophoresis. It ensures that DNA and RNA remain intact and unfragmented, allowing for reliable analysis. The pH balance is particularly significant in experiments where accurate nucleic acid quantification is required, such as in cloning or sequencing workflows.

What safety precautions should be taken while using 50X TAE buffer?

When handling 50X TAE buffer, it's important to wear appropriate personal protective equipment, including gloves and safety goggles, to avoid skin or eye contact. Even though TAE is generally considered non-toxic, potential risks arise from concentrated forms and the nucleic acids it might contain. Proper lab safety protocols should be followed, including testing for spills and ensuring proper disposal of waste materials.

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CephamLifeSciences

Cepham Life Sciences 50X TAE Buffer protects DNA during electrophoresis.

CephamLifeSciences

Item #: 10478227

50X TAE Buffer (Tris-Acetate-EDTA), 1 L -from Cepham Life Sciences

Q: Where can I buy 50X TAE Buffer Tris-Acetate-EDTA, 1 L from Cepham Life Sciences in South Africa?

You can purchase 50X TAE Buffer Tris-Acetate-EDTA, 1 L from Cepham Life Sciences at Ubuy. Ubuy offers a convenient online shopping experience, making it easier for you to acquire essential lab supplies directly from your secure environment. With a straightforward ordering process, you can find and acquire this buffer quickly, ensuring your laboratory experiments continue without a hitch.

Item #: 10478227

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What Stands Out

High Purity

This 50X TAE Buffer is formulated with high purity reagents, ensuring reliable and consistent results for molecular biology applications, minimizing contamination risks.

Convenient Storage

Packaged in a 1 L volume, this buffer is easy to store and use. Its concentrated formulation allows for straightforward dilution, making it user-friendly for laboratory settings.

Versatile Applications

Ideal for DNA electrophoresis, this TAE buffer offers versatility across various molecular biology techniques, addressing multiple experimental needs compared to other buffers on the market.

Product Details

Item Weight

2.2 lbs (1 kg)

Who Should Buy?

Molecular Biologists
Ideal for DNA electrophoresis and gel preparation, aiding in separation and analysis of nucleic acids.
Academic Researchers
Beneficial for university laboratories conducting experiments involving nucleic acids, enhancing reliability in research outcomes.
Genetics Labs
Supports various genetic engineering tests, providing a suitable buffer for PCR and cloning applications.

Non-Scientific Users
Individuals without a background in chemistry or molecular biology may struggle to utilize this product effectively.
General Public
Unsuitable for casual use; requires knowledge and safety precautions in laboratory settings for proper handling.
Low-Resource Labs
Labs with minimal resources may find the concentrated buffer impractical due to dilution requirements for use.

Product Description

50X TAE Buffer (Tris-Acetate-EDTA), 1 L -from Cepham Life Sciences

About This Item

Introducing the Cepham Life Sciences 50X TAE Buffer Tris-Acetate-EDTA, 1L - a vital tool for running nucleic acid electrophoresis in gel formation. Our 50X TAE Buffer is expertly formulated with 2M Tris, 1M Acetic acid, and 0.05M EDTA to provide the optimal electrical conductivity and pH maintenance required for successful electrophoresis. The EDTA in our buffer plays a crucial role in protecting DNA from nucleases by chelating divalent cations, such as Ca2+ and Mg2+.

Whether you're working with genomic or large supercoiled DNA, our TAE Buffer provides consistent results and can even be used as a gel preparation buffer. It's important to note that while TAE has a lower buffering capacity compared to TBE buffer, it enables dsDNA to run faster during electrophoresis. To use our 50X TAE Buffer, simply dilute it with molecular biology grade water to 1X before starting your electrophoresis experiment.

Remember, this product is intended for research use only and is not suitable for human or animal clinical/therapeutic purposes. For your safety, please follow all provided safety instructions carefully. We offer the Cepham Life Sciences 50X TAE Buffer Tris-Acetate-EDTA in convenient sizes of 500 mL, 1 L, and 1 Gallon, allowing you to choose the perfect quantity for your specific needs.

Rest assured that when you choose Cepham Life Sciences, you're selecting a brand trusted by scientists and researchers worldwide. Enhance your nucleic acid electrophoresis process and achieve reliable results with the Cepham Life Sciences 50X TAE Buffer Tris-Acetate-EDTA, 1L. Place your order today and experience the difference of a superior quality buffer solution.

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Customer Questions & Answers

Question: How to Shop 50X TAE Buffer (Tris-Acetate-EDTA), 1 L -from Cepham Online From Ubuy?

Answer: It’s easy to shop 50X TAE Buffer (Tris-Acetate-EDTA), 1 L -from Cepham online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.
Question: Is 50X TAE Buffer (Tris-Acetate-EDTA), 1 L -from Cepham Available to Shop Online in South Africa?

Answer: Yes, at Ubuy South Africa this product is available for you to shop at a reasonable price. The 50X TAE Buffer (Tris-Acetate-EDTA), 1 L -from Cepham is not available locally but you can trust us with our express shipping services.
Question: How Long Does It Take to Get Product After Placing the Order?

Answer: The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

CephamLifeSciences Buffers Editorial Review

Cepham Life Sciences' 50X TAE Buffer Tris-Acetate-EDTA solution comes in a 1 L size and is a reliable and high-quality product for running DNA electrophoresis gels. The buffer solution is clear and free of any debris. It provides excellent band resolution, and its high ionic strength ensures that the DNA molecules do not degrade, resulting in accurate and reproducible results. The solution is easy to prepare, and the 1 L size is a convenient size for most laboratories. Customer Feedback:

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Pros

Clear, high-quality solution
Excellent band resolution
Accurate and reproducible results
Easy to prepare
Convenient 1 L size

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No negative feedback provided Overall, customers had a positive experience with Cepham Life Sciences' 50X TAE Buffer Tris-Acetate-EDTA solution, with no negatives to report. It is a reliable and high-quality product that provides accurate and reproducible results, making it an excellent choice for laboratories conducting DNA electrophoresis gels.

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Features & Benefits

Contains 2M Tris, 1M Acetic acid with 0.05M EDTA.
Inhibits metal dependent nucleases.
Lower buffering capacity than TBE buffer.
Should be diluted with molecular biology grade water before use.
Available in 500 mL, 1 L, and 1 Gal sizes.
For research use only. Not for human or animal clinical/therapeutic use.

50X TAE Buffer (Tris-Acetate-EDTA), 1 L -from Cepham Life Sciences

ZAR 2980

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100% Original Products

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What Stands Out

Product Details

Who Should Buy?

Product Description

About This Item

Customer Questions & Answers

CephamLifeSciences Buffers Editorial Review

Customer Reviews & Ratings

Review this product

Pros

Cons

Product Price History

Important information

ZAR 2980

Features & Benefits

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